##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469514
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01977150841083	33.0	31.0	34.0	30.0	34.0
2	32.071286479210414	33.0	31.0	34.0	30.0	34.0
3	32.056241134449664	33.0	31.0	34.0	30.0	34.0
4	35.73878947166644	37.0	35.0	37.0	33.0	37.0
5	32.442825986019585	37.0	35.0	37.0	19.0	37.0
6	33.96990718061655	37.0	35.0	37.0	19.0	37.0
7	35.48049685419391	37.0	35.0	37.0	32.0	37.0
8	35.487951370992135	37.0	35.0	37.0	33.0	37.0
9	37.41687787797595	39.0	37.0	39.0	34.0	39.0
10	37.15924764756748	39.0	37.0	39.0	34.0	39.0
11	37.24726206247311	39.0	37.0	39.0	34.0	39.0
12	37.2205961909549	39.0	37.0	39.0	34.0	39.0
13	37.180565435748456	39.0	37.0	39.0	34.0	39.0
14	38.30808665982271	40.0	38.0	41.0	34.0	41.0
15	38.4586487303893	40.0	38.0	41.0	34.0	41.0
16	38.255985976988974	40.0	37.0	41.0	34.0	41.0
17	38.257266024016324	40.0	37.0	41.0	34.0	41.0
18	38.154860131966245	40.0	37.0	41.0	34.0	41.0
19	38.00393385500752	40.0	37.0	41.0	34.0	41.0
20	38.037871075196904	40.0	36.0	41.0	34.0	41.0
21	37.99527383634993	40.0	36.0	41.0	34.0	41.0
22	38.119365982697005	40.0	36.0	41.0	34.0	41.0
23	38.12309963068194	40.0	36.0	41.0	34.0	41.0
24	38.0126386007659	40.0	36.0	41.0	34.0	41.0
25	37.78296493821271	40.0	35.0	41.0	34.0	41.0
26	37.8882035466461	40.0	35.0	41.0	34.0	41.0
27	37.88098970424737	40.0	35.0	41.0	34.0	41.0
28	37.78017907879211	40.0	35.0	41.0	34.0	41.0
29	37.79953526412418	40.0	36.0	41.0	34.0	41.0
30	37.53684874146458	40.0	35.0	41.0	33.0	41.0
31	37.470948683106364	40.0	35.0	41.0	33.0	41.0
32	37.309307070715676	40.0	35.0	41.0	33.0	41.0
33	37.10513637506017	40.0	35.0	41.0	32.0	41.0
34	36.95818016076198	40.0	35.0	41.0	32.0	41.0
35	36.81833981521318	40.0	35.0	41.0	31.0	41.0
36	36.699708634886285	40.0	35.0	41.0	31.0	41.0
37	36.688565197203914	40.0	35.0	41.0	31.0	41.0
38	36.56444536265159	40.0	35.0	41.0	30.0	41.0
39	36.48888425052288	40.0	35.0	41.0	30.0	41.0
40	36.38382029076875	39.0	35.0	41.0	30.0	41.0
41	36.283508053008006	39.0	35.0	41.0	30.0	41.0
42	36.35207043879416	39.0	35.0	41.0	30.0	41.0
43	36.29169524231439	39.0	35.0	41.0	30.0	41.0
44	36.2475027368726	39.0	35.0	41.0	30.0	41.0
45	36.17878913088854	39.0	35.0	41.0	30.0	41.0
46	36.08569499525041	39.0	35.0	41.0	29.0	41.0
47	36.0203636100308	39.0	35.0	41.0	29.0	41.0
48	35.97891862649463	39.0	35.0	41.0	29.0	41.0
49	35.94796321302453	39.0	35.0	41.0	29.0	41.0
50	35.85692226429883	39.0	35.0	41.0	29.0	41.0
51	34.949963153388396	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	12.0
11	8.0
12	14.0
13	11.0
14	11.0
15	21.0
16	34.0
17	85.0
18	121.0
19	208.0
20	391.0
21	690.0
22	1118.0
23	1853.0
24	2671.0
25	4196.0
26	5764.0
27	6798.0
28	6675.0
29	6358.0
30	6913.0
31	8238.0
32	10756.0
33	15775.0
34	27834.0
35	36342.0
36	35297.0
37	54147.0
38	90485.0
39	146603.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83243524154764	20.624944091124014	22.08624236976959	16.456378297558754
2	36.85832584331884	24.713001103268486	21.677095890644367	16.751577162768307
3	31.481489369859045	24.368815413384905	28.369548085893072	15.780147130862979
4	28.942267962190694	23.39951524342192	29.92328237283659	17.734934421550793
5	24.20673291957215	33.702296417146236	24.209075767708736	17.88189489557287
6	24.452518987719216	34.0530846790511	27.00238970509932	14.492006628130364
7	73.06512691847314	6.667106838134752	15.283037353518743	4.984728889873359
8	72.85256669662672	8.085807877933354	13.962096976873958	5.099528448565964
9	65.84297805816227	8.782485719275677	16.197813057757596	9.176723164804457
10	38.18480386101373	24.873379707527356	23.381411416911956	13.560405014546955
11	29.590597937441693	24.76177494174828	27.51611240559387	18.13151471521616
12	28.15570994688124	21.374229522442356	30.65063022614874	19.81943030452766
13	24.52940700383801	23.55116141371716	32.59519417951328	19.324237402931544
14	19.191972976311675	27.576174512368105	31.83312957654085	21.398722934779368
15	18.531502787989282	25.653335150815526	36.25025025877823	19.56491180241697
16	20.834522506251144	24.89829909225284	33.87375030350533	20.393428097990686
17	20.684154253121314	24.63206634945923	31.926204543421495	22.757574853997966
18	20.813649859216127	24.939192441545938	32.665266637416565	21.581891061821374
19	22.430002087264704	25.74939192441546	31.054452050418092	20.766153937901745
20	24.58946911061225	25.030776505066942	32.43950127152758	17.94025311279323
21	23.162887581626958	25.719573857222578	32.00692631103652	19.11061225011395
22	21.181690002854015	23.94348198349783	31.592242190861185	23.282585822786967
23	21.01939452284703	25.814139727462866	32.4786907312668	20.687775018423306
24	21.19148736778882	24.27701836366967	32.46484662864153	22.06664763989998
25	20.158504325749604	26.80516448923781	30.712396222476855	22.32393496253573
26	19.923154581120052	26.087188028471996	32.73555208151407	21.254105308893877
27	21.175300417026968	26.4119919746802	32.27869669488024	20.13401091341259
28	18.864187223384178	26.131702143067088	33.834134871377636	21.169975762171095
29	20.357007458776522	24.62439884646677	33.54937232968559	21.469221365071117
30	20.368082740876737	25.07294777152545	34.27395136247268	20.285018125125127
31	22.194439356440913	25.731714070293965	30.180356709278104	21.893489863987018
32	23.17907453238881	26.21348884165328	30.325400307552066	20.28203631840584
33	21.882414581886803	25.698062251604853	30.32050162508466	22.099021541423685
34	21.40404758963524	26.3112495048071	31.071916918345355	21.21278598721231
35	20.406420255839016	25.230983527647737	31.06872212543183	23.293874091081417
36	21.055815162061194	28.147829457694552	30.672993776543407	20.123361603700847
37	22.307960997968113	27.332731292357632	31.02953266569261	19.32977504398165
38	22.334371286053237	26.566193979306263	28.318218413082462	22.781216321558038
39	22.871309481719397	26.391758286227883	29.648956154662052	21.087976077390664
40	21.32950242165303	24.930247021388073	32.442057105858396	21.2981934511005
41	19.137874482976013	27.68884420911836	30.014653450163358	23.15862785774226
42	20.66711535758252	25.692524610554745	30.963506945479796	22.676853086382938
43	23.00911154938937	25.61797944257253	29.794425725324487	21.578483282713616
44	21.36400618511908	25.661428626196447	30.01955213263076	22.955013056053705
45	21.30181421640249	24.628871556545704	29.487086647043537	24.582227580008265
46	21.91500146960474	26.305498877562755	29.821687958186548	21.957811694645955
47	19.600480496854196	26.110829496032068	32.682305532955354	21.606384474158386
48	19.944240214349307	25.5176629450879	31.38181183095712	23.156285009605675
49	20.975732353028874	24.21184458823379	32.94704737238932	21.86537568634801
50	20.06329949692661	24.26487815059828	31.898729324365192	23.77309302810992
51	19.33382178167211	23.86595500879633	29.73031688085978	27.069906328671777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	680.5
2	1077.0
3	5159.5
4	9242.0
5	6277.0
6	3312.0
7	3221.5
8	3131.0
9	2965.0
10	2799.0
11	2743.5
12	2688.0
13	2594.0
14	2500.0
15	2322.0
16	2144.0
17	2176.5
18	2209.0
19	2104.5
20	2000.0
21	2484.5
22	2969.0
23	2927.5
24	2886.0
25	3219.5
26	4222.0
27	4891.0
28	5296.0
29	5701.0
30	6966.5
31	8232.0
32	8683.5
33	9135.0
34	10134.5
35	11134.0
36	12614.5
37	14095.0
38	15804.5
39	17514.0
40	19953.0
41	22392.0
42	24972.0
43	27552.0
44	35248.5
45	42945.0
46	43478.0
47	44011.0
48	43104.5
49	42198.0
50	37377.5
51	32557.0
52	29938.5
53	27320.0
54	24907.0
55	22494.0
56	21168.5
57	19843.0
58	19141.5
59	18440.0
60	18025.0
61	17610.0
62	15706.0
63	13802.0
64	11943.0
65	10084.0
66	8057.5
67	6031.0
68	5077.5
69	4124.0
70	3468.5
71	2813.0
72	2266.0
73	1719.0
74	1385.0
75	777.5
76	504.0
77	409.5
78	315.0
79	204.0
80	93.0
81	64.5
82	36.0
83	53.0
84	70.0
85	38.5
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.587471954705826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59550250940656	26.190778815459886
2	10.12694822691234	7.207849720240017
3	3.7415305882197214	3.9945484462783
4	1.9416382533861107	2.763919875542489
5	1.1861022545772024	2.110519036008977
6	0.8939985414005962	1.9089088811784987
7	0.760802012042781	1.8952514186659362
8	0.6396680318275255	1.8211334514387154
9	0.5705679968734256	1.8274565328287147
>10	6.416475469004783	42.10977990506144
>50	0.07700745385686696	1.9200368850146106
>100	0.04442737722511555	3.037950323885107
>500	0.0023694601186728293	0.5946886311622988
>1k	0.0029618251483410365	2.617178077235001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4763	1.0144532431407796	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	2083	0.4436502425912752	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	2064	0.43960350490081235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	1908	0.40637765860016956	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1597	0.34013895219311885	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	859	0.18295514084777023	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	718	0.15292408746065078	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT	654	0.13929297102961785	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	590	0.12566185459858492	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.10415024898086107	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC	485	0.10329830420392151	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.129861942348897E-4	0.0	0.0	0.1588877008992277	0.0
2	2.129861942348897E-4	0.0	0.0	0.8470460944721563	0.0
3	2.129861942348897E-4	0.0	0.0	1.1337255119123177	0.0
4	2.129861942348897E-4	0.0	0.0	1.569495265316902	0.0
5	2.129861942348897E-4	0.0	0.0	2.989261236086677	0.0
6	2.129861942348897E-4	0.0	0.0	3.6133107851949036	0.0
7	2.129861942348897E-4	0.0	0.0	4.289754938084913	0.0
8	2.129861942348897E-4	0.0	0.0	5.300374429729465	0.0
9	2.129861942348897E-4	0.0	0.0	5.65819123604408	0.0
10	2.129861942348897E-4	0.0	0.0	6.922477285022385	0.0
11	2.129861942348897E-4	0.0	0.0	8.267059129227244	0.0
12	2.129861942348897E-4	0.0	0.0	9.763074157533108	0.0
13	2.129861942348897E-4	0.0	0.0	10.227597047159403	0.0
14	2.129861942348897E-4	0.0	0.0	10.433128724596072	0.0
15	2.129861942348897E-4	0.0	0.0	10.906810020574467	0.0
16	2.129861942348897E-4	0.0	0.0	11.669300595935372	0.0
17	2.129861942348897E-4	0.0	0.0	12.554897191564043	0.0
18	4.259723884697794E-4	0.0	0.0	13.46945990960866	0.0
19	4.259723884697794E-4	0.0	0.0	14.176787060662727	0.0
20	4.259723884697794E-4	0.0	0.0	14.72309664887522	0.0
21	4.259723884697794E-4	0.0	0.0	15.389317464441955	0.0
22	4.259723884697794E-4	0.0	0.0	16.066613562108905	0.0
23	4.259723884697794E-4	0.0	0.0	16.707276034367453	0.0
24	4.259723884697794E-4	0.0	0.0	17.226110403523645	0.0
25	4.259723884697794E-4	0.0	0.0	17.687012527847944	0.0
26	4.259723884697794E-4	0.0	0.0	18.15515618277623	0.0
27	4.259723884697794E-4	0.0	0.0	18.594333715288574	0.0
28	4.259723884697794E-4	0.0	0.0	19.050337157145474	0.0
29	4.259723884697794E-4	0.0	0.0	19.52721324603739	0.0
30	4.259723884697794E-4	0.0	0.0	20.036676222647248	0.0
31	6.38958582704669E-4	0.0	0.0	20.517599049229627	0.0
32	6.38958582704669E-4	0.0	0.0	20.97679728400005	0.0
33	6.38958582704669E-4	2.129861942348897E-4	0.0	21.4215124575625	0.0
34	6.38958582704669E-4	2.129861942348897E-4	0.0	21.891572988238902	0.0
35	6.38958582704669E-4	2.129861942348897E-4	0.0	22.379950331619504	0.0
36	6.38958582704669E-4	2.129861942348897E-4	0.0	22.864706909698114	0.0
37	6.38958582704669E-4	2.129861942348897E-4	0.0	23.355640087409533	0.0
38	6.38958582704669E-4	2.129861942348897E-4	0.0	23.81313443262608	0.0
39	6.38958582704669E-4	2.129861942348897E-4	0.0	24.3089662928049	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	20	7.02902E-4	45.0	44
GCGCGAC	25	3.8872684E-5	45.0	9
CAACCGG	20	7.02902E-4	45.0	1
CGACCCG	20	7.02902E-4	45.0	32
CGACAAT	25	3.8872684E-5	45.0	20
AACGGCA	20	7.02902E-4	45.0	20
GCCGATC	20	7.02902E-4	45.0	43
GTATGCG	25	3.8872684E-5	45.0	1
TACGAAT	50	2.1827873E-11	45.0	12
TCATCGA	20	7.02902E-4	45.0	16
TTCGGAC	25	3.8872684E-5	45.0	14
ACGTAAG	35	1.2098826E-7	45.0	1
CGCACGG	35	1.2098826E-7	45.0	2
CAATACA	20	7.02902E-4	45.0	1
TTACGGG	115	0.0	43.04348	3
CGTTTTT	2500	0.0	42.390003	1
GTATTAG	60	3.6379788E-12	41.249996	1
CGAATAT	50	1.0786607E-9	40.5	14
CGTTATT	245	0.0	40.408165	1
CCTCGTC	45	1.924127E-8	40.0	36
>>END_MODULE