FastQCFastQC Report
Sat 18 Jun 2016
SRR3554438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401438
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56121.3979742824545758No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG37900.9441059391487603No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC37280.928661462043952No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC35340.8803351949740683No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC19330.481518939412811TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT11660.2904558113581674No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT7640.1903158146463464No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC7510.1870774565437253TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA7380.18383909844110424No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.1482171593122724No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT5860.14597521908738087No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC5860.14597521908738087No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT5800.14448059227078652TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAT453.8380676E-1045.00000443
ACAACGG253.886255E-545.02
CGGAATT207.0277945E-445.021
TAATACG1000.045.04
CGCATAC207.0277945E-445.026
ACGGGCC551.8189894E-1245.05
GCCGATT351.2092823E-745.09
GTATGCG351.2092823E-745.01
TACGAAT253.886255E-545.012
TTGCGGA207.0277945E-445.018
CGAATAT253.886255E-545.014
AACGACG207.0277945E-445.013
CGGCTAA207.0277945E-445.040
CGCAATC207.0277945E-445.020
TTCGCGG253.886255E-545.02
CTGATCG207.0277945E-445.017
TCGAATG406.7957444E-945.044
ACGACGC207.0277945E-445.014
ATATGCG302.1619599E-644.9999961
CACCCGT302.1619599E-644.99999638
Produced by FastQC (version 0.11.5)