##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401438
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90492429715174	33.0	31.0	34.0	30.0	34.0
2	31.95307619109302	33.0	31.0	34.0	30.0	34.0
3	31.95054528968359	33.0	31.0	34.0	30.0	34.0
4	35.67908369411964	37.0	35.0	37.0	33.0	37.0
5	32.300870869225136	37.0	35.0	37.0	16.0	37.0
6	33.863699998505375	37.0	35.0	37.0	19.0	37.0
7	35.4095925149089	37.0	35.0	37.0	32.0	37.0
8	35.42596864272939	37.0	35.0	37.0	33.0	37.0
9	37.30940269730319	39.0	37.0	39.0	34.0	39.0
10	37.035813749570295	39.0	37.0	39.0	33.0	39.0
11	37.105373681614594	39.0	37.0	39.0	34.0	39.0
12	37.09222345667326	39.0	37.0	39.0	34.0	39.0
13	37.06088362337397	39.0	37.0	39.0	33.0	39.0
14	38.15450455612075	40.0	37.0	41.0	33.0	41.0
15	38.300106118503976	40.0	37.0	41.0	34.0	41.0
16	38.15252168454406	40.0	37.0	41.0	33.0	41.0
17	38.09735749978826	40.0	37.0	41.0	33.0	41.0
18	37.982908942352246	39.0	37.0	41.0	33.0	41.0
19	37.7879398562169	39.0	37.0	41.0	33.0	41.0
20	37.70014049492076	39.0	35.0	41.0	33.0	41.0
21	37.716932627205196	39.0	35.0	41.0	33.0	41.0
22	37.81564525530717	40.0	35.0	41.0	34.0	41.0
23	37.786305730897425	40.0	35.0	41.0	34.0	41.0
24	37.69087380865788	39.0	35.0	41.0	33.0	41.0
25	37.497870156786355	39.0	35.0	41.0	33.0	41.0
26	37.57131860959849	39.0	35.0	41.0	33.0	41.0
27	37.52543107528435	39.0	35.0	41.0	33.0	41.0
28	37.411044793965694	39.0	35.0	41.0	33.0	41.0
29	37.416415486326656	39.0	35.0	41.0	33.0	41.0
30	37.13395592843727	39.0	35.0	41.0	33.0	41.0
31	37.044427782123265	39.0	35.0	41.0	32.0	41.0
32	36.74748279933639	39.0	35.0	41.0	31.0	41.0
33	36.49375495095133	39.0	35.0	41.0	30.0	41.0
34	36.28235244296753	40.0	35.0	41.0	30.0	41.0
35	36.082916913695264	39.0	35.0	41.0	29.0	41.0
36	35.94623329131771	39.0	35.0	41.0	27.0	41.0
37	35.854092039119365	39.0	35.0	41.0	27.0	41.0
38	35.816061259771125	39.0	35.0	41.0	26.0	41.0
39	35.75772597511945	39.0	35.0	41.0	26.0	41.0
40	35.69847896810965	39.0	35.0	41.0	25.0	41.0
41	35.56726817092552	39.0	35.0	41.0	24.0	41.0
42	35.54686153278962	39.0	35.0	41.0	24.0	41.0
43	35.45422954478649	39.0	35.0	41.0	24.0	41.0
44	35.448963476302694	39.0	35.0	41.0	24.0	41.0
45	35.38767630368824	39.0	35.0	41.0	24.0	41.0
46	35.28936473378205	39.0	35.0	41.0	23.0	41.0
47	35.166187555737125	38.0	35.0	41.0	23.0	41.0
48	35.14036787748046	38.0	35.0	40.0	23.0	41.0
49	35.11886019758966	38.0	35.0	40.0	24.0	41.0
50	35.02448198725582	38.0	35.0	40.0	24.0	41.0
51	34.10691314723569	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	5.0
12	5.0
13	10.0
14	12.0
15	12.0
16	27.0
17	63.0
18	131.0
19	265.0
20	442.0
21	913.0
22	1575.0
23	2356.0
24	3238.0
25	5005.0
26	6664.0
27	7531.0
28	7150.0
29	6798.0
30	6791.0
31	8044.0
32	10436.0
33	14622.0
34	24807.0
35	31372.0
36	29979.0
37	44968.0
38	74383.0
39	113767.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.791320203867095	16.334527374090147	20.58126036897354	19.292892053069217
2	34.74235124726608	27.116267020062875	22.125708079454363	16.015673653216687
3	28.393674739312175	26.171164663036382	30.401705867406676	15.033454730244769
4	26.226465855250375	22.34018702763565	34.48303349458696	16.950313622527016
5	24.706430382773927	32.09437073719977	26.55030166551248	16.648897214513823
6	23.258386101963442	33.86824366402782	29.921432450341023	12.951937783667713
7	69.43612712299284	5.60310683094276	20.300270527453804	4.6604955186105945
8	69.19349936976569	9.009112241491838	16.403031103184055	5.394357285558417
9	63.10189867426601	7.143319765443231	18.36248685973924	11.392294700551517
10	38.08010203319068	22.436341352836553	25.37328304744444	14.110273566528331
11	29.28422321753297	22.167059421380138	31.25364315286545	17.295074208221443
12	25.434313642455376	20.14881501003891	34.30243275424847	20.11443859325724
13	23.572257733448254	21.838490626198816	37.05852460404845	17.530727036304487
14	18.16569432888765	27.619208943846868	34.42972513812843	19.785371589137053
15	17.93876015723474	22.232075687901993	40.80978880923081	19.019375345632454
16	20.187924411739797	22.79056790836941	35.31952630294093	21.701981376949867
17	20.027501133425336	24.606041281592674	34.50894035940793	20.857517225574064
18	20.470159775606696	22.75370044689342	35.57062360812878	21.205516169371112
19	20.95765722228588	24.707177696182224	32.49966370896627	21.835501372565627
20	23.18415297007259	23.794209815712513	35.28539899062869	17.736238223586206
21	21.510420039956358	26.28550361450585	34.17439305696023	18.029683288577562
22	19.837932632187286	21.893791818412804	35.06543974411989	23.202835805280017
23	20.106218145765972	24.213203533297794	35.17728765089503	20.503290670041203
24	21.272774376117855	22.458262546146603	34.99693601502598	21.272027062709558
25	18.832048784619293	26.7480906142418	32.57290042297939	21.846960178159517
26	18.273556564151875	24.64091590731321	35.21266048555443	21.872867042980484
27	21.037370652504247	25.006352163970526	33.962654257942695	19.993622925582528
28	18.016480751697646	25.847328852774275	35.40347450913964	20.732715886388434
29	21.2431309442554	23.335857591956916	34.47630767391229	20.944703789875398
30	20.563075742704974	24.651876503968236	35.08586631061335	19.699181442713446
31	23.12561341975598	23.58022907647009	30.807247943642608	22.486909560131327
32	23.539874152422044	26.139777499887902	31.370473149029237	18.949875198660816
33	20.954917073122125	25.175244994245688	30.29459094555075	23.575246987081442
34	21.926673608377882	24.1855529371908	30.77860092965788	23.109172524773438
35	20.114936802196105	24.870839332599306	32.74503161135717	22.269192253847418
36	22.47794179923176	27.775895655119843	29.54702843278414	20.199134112864254
37	20.163761278204852	27.27071179111096	32.96573817127427	19.599788759409922
38	20.93150125299548	26.83677180535973	28.590716374633196	23.641010567011595
39	21.13153214194969	26.630015095730847	29.01743233077088	23.221020431548585
40	20.70107961877052	22.982378349832352	32.28667938760157	24.029862643795553
41	18.728919534274283	26.213014213901026	30.45974720878442	24.59831904304027
42	20.82613006242558	24.95752768796178	32.85538489131572	21.360957358296922
43	22.40694702544353	25.425345881555806	30.084595877819243	22.08311121518142
44	21.557251680209646	24.15416577404232	30.516792132284436	23.7717904134636
45	21.272525271648423	23.013267304041968	29.646670220557098	26.06753720375251
46	22.43335209920336	25.36431528654487	30.3942327333237	21.808099880928065
47	18.843258485743753	24.257045919917893	34.78444990260015	22.1152456917382
48	20.23126858942103	24.285194724963755	31.751852091730232	23.73168459388498
49	19.978178448477724	22.901170292797392	34.626517668980014	22.494133589744866
50	19.851135169067202	23.812394441981077	31.970565815891867	24.36590457305985
51	19.29339026200808	22.507585231094215	30.760416303389317	27.438608203508387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	472.5
2	795.0
3	5828.5
4	10862.0
5	7168.0
6	3474.0
7	3400.5
8	3327.0
9	3323.0
10	3319.0
11	3186.0
12	3053.0
13	2916.5
14	2780.0
15	2596.5
16	2413.0
17	2285.5
18	2158.0
19	2056.5
20	1955.0
21	2125.0
22	2295.0
23	2178.0
24	2061.0
25	2389.5
26	3284.5
27	3851.0
28	4106.5
29	4362.0
30	5179.5
31	5997.0
32	6958.0
33	7919.0
34	9384.0
35	10849.0
36	11045.0
37	11241.0
38	12033.5
39	12826.0
40	14821.5
41	16817.0
42	18796.5
43	20776.0
44	23997.5
45	27219.0
46	35455.0
47	43691.0
48	40450.0
49	37209.0
50	36300.5
51	35392.0
52	30681.5
53	25971.0
54	23481.0
55	20991.0
56	19230.5
57	17470.0
58	16013.5
59	14557.0
60	14503.0
61	14449.0
62	12392.5
63	10336.0
64	8602.5
65	6869.0
66	5528.0
67	4187.0
68	3532.0
69	2877.0
70	2364.5
71	1852.0
72	1402.5
73	953.0
74	806.5
75	548.0
76	436.0
77	311.0
78	186.0
79	127.0
80	68.0
81	45.5
82	23.0
83	28.5
84	34.0
85	20.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.28916071309231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28452918333535	28.825814371102148
2	9.558522168699483	7.319755829939803
3	3.533466636813927	4.058804157939547
4	1.9159218146969261	2.9343615310660027
5	1.1499838954204944	2.2015959094611626
6	0.887896478412164	2.0398086575107226
7	0.7415685851381882	1.9875827115295595
8	0.6690117163603668	2.049271770133105
9	0.5940697515692779	2.0471788973360616
>10	5.577073170062585	37.45840912084725
>50	0.05367939527774795	1.4308130964135624
>100	0.025869588085661664	1.6135649249274444
>500	0.004527177914990791	1.139103538162407
>1k	0.003233698510707708	3.504229167073091
>5k	6.467397021415415E-4	1.3897063165581365
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5612	1.3979742824545758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	3790	0.9441059391487603	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	3728	0.928661462043952	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	3534	0.8803351949740683	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1933	0.481518939412811	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	1166	0.2904558113581674	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	764	0.1903158146463464	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	751	0.1870774565437253	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA	738	0.18383909844110424	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	595	0.1482171593122724	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	586	0.14597521908738087	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	586	0.14597521908738087	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	580	0.14448059227078652	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.491044694323906E-4	0.0	0.0	0.15245193529262302	0.0
2	2.491044694323906E-4	0.0	0.0	0.9886956391771581	0.0
3	2.491044694323906E-4	0.0	0.0	1.2422839890593318	0.0
4	2.491044694323906E-4	0.0	0.0	1.7464714351904902	0.0
5	2.491044694323906E-4	0.0	0.0	3.6147549559334196	0.0
6	2.491044694323906E-4	0.0	0.0	4.39221000503191	0.0
7	2.491044694323906E-4	0.0	0.0	5.20703072454526	0.0
8	2.491044694323906E-4	0.0	0.0	6.346932776667879	0.0
9	2.491044694323906E-4	0.0	0.0	6.717102018244411	0.0
10	2.491044694323906E-4	0.0	0.0	8.614779866380363	0.0
11	2.491044694323906E-4	0.0	0.0	9.895675048201715	0.0
12	2.491044694323906E-4	0.0	0.0	11.855878118165196	0.0
13	2.491044694323906E-4	0.0	0.0	12.323696311759226	0.0
14	2.491044694323906E-4	0.0	0.0	12.546644811901215	0.0
15	2.491044694323906E-4	0.0	0.0	13.097913002755096	0.0
16	2.491044694323906E-4	0.0	0.0	13.875368051853586	0.0
17	2.491044694323906E-4	0.0	0.0	14.738016829497955	0.0
18	2.491044694323906E-4	0.0	0.0	15.632800083699102	0.0
19	2.491044694323906E-4	0.0	0.0	16.519611994878414	0.0
20	2.491044694323906E-4	0.0	0.0	17.06639630528251	0.0
21	2.491044694323906E-4	0.0	0.0	17.715064343684453	0.0
22	2.491044694323906E-4	0.0	0.0	18.41803715642266	0.0
23	2.491044694323906E-4	0.0	0.0	19.078911313826794	0.0
24	2.491044694323906E-4	0.0	0.0	19.60053607281822	0.0
25	2.491044694323906E-4	0.0	0.0	20.052660684838006	0.0
26	2.491044694323906E-4	0.0	0.0	20.49656484936653	0.0
27	2.491044694323906E-4	0.0	0.0	20.969365132349203	0.0
28	2.491044694323906E-4	0.0	0.0	21.413518401347158	0.0
29	2.491044694323906E-4	0.0	0.0	21.889806146901886	0.0
30	2.491044694323906E-4	0.0	0.0	22.394990010910774	0.0
31	2.491044694323906E-4	0.0	0.0	22.88821686038691	0.0
32	2.491044694323906E-4	0.0	0.0	23.35703147185867	0.0
33	2.491044694323906E-4	0.0	0.0	23.808159666000726	0.0
34	2.491044694323906E-4	0.0	0.0	24.250569203712654	0.0
35	2.491044694323906E-4	0.0	0.0	24.721127546470438	0.0
36	2.491044694323906E-4	0.0	0.0	25.17947977022604	0.0
37	2.491044694323906E-4	0.0	0.0	25.667973634782957	0.0
38	2.491044694323906E-4	0.0	0.0	26.129564216641175	0.0
39	2.491044694323906E-4	0.0	0.0	26.631011513608577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGAAT	45	3.8380676E-10	45.000004	43
ACAACGG	25	3.886255E-5	45.0	2
CGGAATT	20	7.0277945E-4	45.0	21
TAATACG	100	0.0	45.0	4
CGCATAC	20	7.0277945E-4	45.0	26
ACGGGCC	55	1.8189894E-12	45.0	5
GCCGATT	35	1.2092823E-7	45.0	9
GTATGCG	35	1.2092823E-7	45.0	1
TACGAAT	25	3.886255E-5	45.0	12
TTGCGGA	20	7.0277945E-4	45.0	18
CGAATAT	25	3.886255E-5	45.0	14
AACGACG	20	7.0277945E-4	45.0	13
CGGCTAA	20	7.0277945E-4	45.0	40
CGCAATC	20	7.0277945E-4	45.0	20
TTCGCGG	25	3.886255E-5	45.0	2
CTGATCG	20	7.0277945E-4	45.0	17
TCGAATG	40	6.7957444E-9	45.0	44
ACGACGC	20	7.0277945E-4	45.0	14
ATATGCG	30	2.1619599E-6	44.999996	1
CACCCGT	30	2.1619599E-6	44.999996	38
>>END_MODULE