FastQCFastQC Report
Sat 18 Jun 2016
SRR3554437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519356
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC71841.3832515654002264TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG57561.108295658469335No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC51900.9993145356942059No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.6617811289366061No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC23020.4432412449264089TruSeq Adapter, Index 13 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT18030.34716071442324725TruSeq Adapter, Index 13 (100% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT13110.2524280069932763No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT12610.24280069932762885No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC9570.1842666687204923No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA9060.1744468149015319No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT8410.1619313149361902No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT6700.1290059227196759TruSeq Adapter, Index 13 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC6460.12438481504016513TruSeq Adapter, Index 13 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG302.163195E-645.00000420
GCGATAT302.163195E-645.0000049
CGACAAA351.21021E-745.012
TAGCACG207.029712E-445.01
ACGATAG207.029712E-445.01
CCCGTAA207.029712E-445.041
ACGGCCA351.21021E-745.030
AGCGCGA207.029712E-445.09
CGATGCG253.887842E-545.010
CGCCCAC207.029712E-445.034
GACTACG253.887842E-545.01
CTCGAAT900.042.543
CGTTTTT18450.042.195121
TACGGGA2900.041.8965534
CCGATGA9050.041.76795618
CGATGAA9600.041.25000419
CGAATAT501.0786607E-940.514
GCTACGA501.0786607E-940.510
ATTGCGG900.040.02
CCACCGA10300.039.97572715
Produced by FastQC (version 0.11.5)