##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519356
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88145703525135	33.0	31.0	34.0	30.0	34.0
2	31.90811505017753	33.0	31.0	34.0	30.0	34.0
3	31.977146697063286	33.0	31.0	34.0	30.0	34.0
4	35.65875622886806	37.0	35.0	37.0	33.0	37.0
5	35.42482420536203	37.0	35.0	37.0	33.0	37.0
6	35.572277975030616	37.0	35.0	37.0	33.0	37.0
7	35.90961691017337	37.0	35.0	37.0	35.0	37.0
8	35.75208527484038	37.0	35.0	37.0	35.0	37.0
9	37.60489529340183	39.0	37.0	39.0	35.0	39.0
10	37.1690978827625	39.0	37.0	39.0	34.0	39.0
11	37.14166775776154	39.0	37.0	39.0	34.0	39.0
12	37.16525273608084	39.0	37.0	39.0	34.0	39.0
13	37.16956962083812	39.0	37.0	39.0	33.0	39.0
14	38.3683658222876	40.0	38.0	41.0	34.0	41.0
15	38.425284390668445	40.0	38.0	41.0	34.0	41.0
16	38.411097590092346	40.0	38.0	41.0	34.0	41.0
17	38.350832569566926	40.0	38.0	41.0	34.0	41.0
18	38.274878888469566	40.0	38.0	41.0	34.0	41.0
19	38.20041166367578	40.0	37.0	41.0	34.0	41.0
20	38.08795315737182	40.0	37.0	41.0	34.0	41.0
21	38.12068985435809	40.0	37.0	41.0	34.0	41.0
22	38.16276118885697	40.0	37.0	41.0	34.0	41.0
23	38.05859757083773	40.0	37.0	41.0	34.0	41.0
24	38.05723819499534	40.0	37.0	41.0	33.0	41.0
25	37.937801816095316	40.0	36.0	41.0	33.0	41.0
26	37.93136307272853	40.0	37.0	41.0	33.0	41.0
27	37.86900315005507	40.0	37.0	41.0	33.0	41.0
28	37.77695453600228	40.0	36.0	41.0	33.0	41.0
29	37.73324848466177	40.0	36.0	41.0	33.0	41.0
30	37.61012099600274	40.0	36.0	41.0	33.0	41.0
31	37.571937168339254	40.0	36.0	41.0	33.0	41.0
32	37.33946079375226	40.0	36.0	41.0	32.0	41.0
33	37.22078497215782	40.0	36.0	41.0	32.0	41.0
34	37.0868710479902	40.0	36.0	41.0	31.0	41.0
35	36.943751492232686	40.0	36.0	41.0	31.0	41.0
36	36.86783632036599	40.0	36.0	41.0	31.0	41.0
37	36.852592441408206	40.0	36.0	41.0	31.0	41.0
38	36.7937349332635	40.0	36.0	41.0	30.0	41.0
39	36.697432974684034	40.0	36.0	41.0	30.0	41.0
40	36.582683169155644	40.0	35.0	41.0	30.0	41.0
41	36.601849213256415	40.0	35.0	41.0	30.0	41.0
42	36.545321513566805	40.0	35.0	41.0	30.0	41.0
43	36.49629541201026	40.0	35.0	41.0	30.0	41.0
44	36.51528623911152	40.0	35.0	41.0	30.0	41.0
45	36.473915772610695	40.0	35.0	41.0	30.0	41.0
46	36.339753463905296	39.0	35.0	41.0	30.0	41.0
47	36.22881029582791	39.0	35.0	41.0	30.0	41.0
48	36.18205238795739	39.0	35.0	41.0	29.0	41.0
49	36.217346097859654	39.0	35.0	41.0	29.0	41.0
50	36.12741549149331	39.0	35.0	41.0	29.0	41.0
51	35.36094509353892	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	3.0
11	9.0
12	4.0
13	12.0
14	24.0
15	15.0
16	40.0
17	82.0
18	129.0
19	298.0
20	613.0
21	1194.0
22	2051.0
23	3107.0
24	3873.0
25	5014.0
26	6101.0
27	6403.0
28	6161.0
29	6051.0
30	6932.0
31	8688.0
32	11394.0
33	15253.0
34	24707.0
35	33132.0
36	34913.0
37	51785.0
38	95674.0
39	195480.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21542063632653	19.327975415707144	22.21674535386132	13.239858594105005
2	31.729102965980942	29.02190405040088	22.934749959565305	16.314243024052864
3	29.18691610379008	28.855929266245116	26.902163448578627	15.054991181386178
4	25.33541539907116	23.951586195210993	32.69992067098483	18.013077734733017
5	28.77756298184675	27.590708492825733	25.26301804542549	18.36871047990203
6	23.798512003327197	36.85525920563159	25.023875723010807	14.322353068030408
7	76.04629579710257	4.537927741279585	15.397338241976602	4.018438219641248
8	76.48414574973621	9.271289828171811	10.022219826092314	4.222344595999661
9	70.81751245773611	7.098984126495121	11.184620953642588	10.898882462126172
10	34.65715231941096	34.9669590800915	17.960127542571954	12.415761057925586
11	25.74111014410154	23.86455533391354	32.2711203875569	18.12321413442802
12	21.77985813199424	22.258527869130234	33.87714785234021	22.084466146535327
13	22.830967582929627	23.57650628855737	35.89657190828642	17.69595422022659
14	18.72819414813731	29.96056654780151	30.716502745708148	20.594736558353038
15	17.007409175979483	26.19282341977372	36.07140381549458	20.728363588752224
16	20.18942690562928	26.724635895224086	30.569590030730364	22.51634716841627
17	20.603978773712058	27.523124793012887	30.727862968753612	21.145033464521447
18	20.4930721894038	25.354284922095825	31.840202096442518	22.312440792057856
19	20.05830297522316	26.78817612581736	29.791125932886114	23.362394966073367
20	23.351612381487843	27.082964286539486	31.628401327798272	17.9370220041744
21	21.477368125139595	28.96240728902718	31.752208504378498	17.808016081454724
22	20.742997096404007	22.74836528315837	33.50996233797241	22.998675282465207
23	20.50616532782908	27.183665924722156	32.04795939586719	20.262209351581575
24	20.10720969816465	27.005368186754364	31.05692434476544	21.830497770315542
25	18.895516755366263	32.01330108827086	29.0504008810912	20.040781275271684
26	19.609285345697362	26.982070102203497	31.608569074007036	21.800075478092097
27	22.448185830143487	26.89715724859249	28.94738868906877	21.707268232195258
28	17.52420305147144	28.595991959272638	31.580457335623347	22.299347653632577
29	19.507235884441503	26.78047427968484	31.275849321082262	22.436440514791396
30	19.323931946487573	29.211947103720764	29.389474657075297	22.074646292716363
31	20.466500820246615	26.036283397130294	27.092784140358443	26.404431642264655
32	21.754056947450305	29.73355463304554	27.69949706944755	20.81289135005661
33	20.468041189473116	27.664453669544592	26.812051848828165	25.055453292154127
34	19.030876701145264	27.592248862052234	28.244595229476506	25.132279207325997
35	19.36417409252998	26.67784717996904	29.32516424186878	24.632814485632206
36	18.951355139827015	28.89366831229446	27.184051017028782	24.970925530849744
37	18.439182372014574	27.284752655211452	29.379269710949714	24.89679526182426
38	18.413958825930578	28.527830620999854	27.133026286400852	25.925184266668722
39	20.339420359060068	28.024900068546433	27.52755335453908	24.10812621785442
40	20.194240559462102	26.557313287995132	30.776192053235164	22.472254099307605
41	18.621523579201934	26.224400988917047	28.15987492201881	26.994200509862214
42	19.516670645953834	25.42648972958818	31.201719052056774	23.855120572401205
43	21.018338095641525	26.123891896887685	27.296497970563543	25.561272036907244
44	20.55892297383683	25.84681798227035	28.358004913777833	25.236254130114986
45	19.723657760765256	25.268024245411624	28.37167569066305	26.63664230316007
46	22.783408683061328	27.20041744006038	27.157287101718282	22.858886775160006
47	18.254530610987455	26.407319834564348	32.18967336470552	23.14847618974268
48	19.66416099939155	26.23479848119594	28.98320227358498	25.117838245827524
49	19.954135506280856	24.42216899390784	32.15713306479563	23.46656243501567
50	19.870570475743037	25.008664576899083	30.14983941650814	24.970925530849744
51	19.712875176179733	24.25099546361263	28.516277851801075	27.519851508406568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	366.5
2	610.0
3	3141.0
4	5672.0
5	3841.0
6	2010.0
7	1950.0
8	1890.0
9	1921.0
10	1952.0
11	1956.5
12	1961.0
13	1891.0
14	1821.0
15	1701.0
16	1581.0
17	1705.5
18	1830.0
19	1835.0
20	1840.0
21	2077.5
22	2315.0
23	2545.5
24	2776.0
25	3149.0
26	4627.5
27	5733.0
28	6848.5
29	7964.0
30	8871.5
31	9779.0
32	11351.0
33	12923.0
34	14865.0
35	16807.0
36	18216.0
37	19625.0
38	20777.0
39	21929.0
40	24012.5
41	26096.0
42	28388.5
43	30681.0
44	33225.5
45	35770.0
46	50710.5
47	65651.0
48	56922.0
49	48193.0
50	46707.0
51	45221.0
52	39633.5
53	34046.0
54	31146.5
55	28247.0
56	24985.0
57	21723.0
58	19640.5
59	17558.0
60	15208.5
61	12859.0
62	11331.0
63	9803.0
64	8389.5
65	6976.0
66	5633.5
67	4291.0
68	3637.0
69	2983.0
70	2415.5
71	1848.0
72	1523.0
73	1198.0
74	965.5
75	596.0
76	459.0
77	302.0
78	145.0
79	122.0
80	99.0
81	75.5
82	52.0
83	53.0
84	54.0
85	29.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.714846117459075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72883165726626	24.900553531671253
2	8.499013658701246	5.9008390262399315
3	3.5588511136843746	3.7063490629950278
4	2.2857592403626006	3.173991211629913
5	1.8051168201512684	3.133217631779417
6	1.514386410766294	3.1543014727273846
7	1.3530053056541318	3.2878559687322215
8	1.1437348196516277	3.176366261070885
9	1.0824910014008027	3.382065768344674
>10	6.935582695959804	36.84355822565321
>50	0.05861595222853491	1.3722598739225127
>100	0.02735411103998243	1.7160515723179874
>500	0.0027912358204063704	0.7790544687428921
>1k	0.0027912358204063704	1.9600390290710474
>5k	0.0016747414922438223	3.5134968951016505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	7184	1.3832515654002264	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	5756	1.108295658469335	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	5190	0.9993145356942059	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3437	0.6617811289366061	No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	2302	0.4432412449264089	TruSeq Adapter, Index 13 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1803	0.34716071442324725	TruSeq Adapter, Index 13 (100% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	1311	0.2524280069932763	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT	1261	0.24280069932762885	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	957	0.1842666687204923	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA	906	0.1744468149015319	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	841	0.1619313149361902	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	670	0.1290059227196759	TruSeq Adapter, Index 13 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	646	0.12438481504016513	TruSeq Adapter, Index 13 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9254615331294912E-4	0.0	0.0	0.13574503808562913	0.0
2	1.9254615331294912E-4	0.0	0.0	0.8583707514691271	0.0
3	1.9254615331294912E-4	0.0	0.0	1.1454570660587342	0.0
4	1.9254615331294912E-4	0.0	0.0	1.8143624026679195	0.0
5	1.9254615331294912E-4	0.0	0.0	4.02902825807346	0.0
6	1.9254615331294912E-4	0.0	0.0	4.816927117430048	0.0
7	1.9254615331294912E-4	0.0	0.0	5.586726638375218	0.0
8	1.9254615331294912E-4	0.0	0.0	6.584115712536295	0.0
9	1.9254615331294912E-4	0.0	0.0	6.9451397499980745	0.0
10	1.9254615331294912E-4	0.0	0.0	8.770669829558145	0.0
11	1.9254615331294912E-4	0.0	0.0	9.710680150031962	0.0
12	1.9254615331294912E-4	0.0	0.0	11.500974283535763	0.0
13	1.9254615331294912E-4	0.0	0.0	11.932085120803457	0.0
14	1.9254615331294912E-4	0.0	0.0	12.134836220241992	0.0
15	1.9254615331294912E-4	0.0	0.0	12.55323901139103	0.0
16	1.9254615331294912E-4	0.0	0.0	13.070610525342925	0.0
17	1.9254615331294912E-4	0.0	0.0	13.71525504663468	0.0
18	1.9254615331294912E-4	0.0	0.0	14.374918167884841	0.0
19	1.9254615331294912E-4	0.0	0.0	15.034003650675066	0.0
20	3.8509230662589825E-4	0.0	0.0	15.487834934033687	0.0
21	3.8509230662589825E-4	0.0	0.0	15.972858694229007	0.0
22	3.8509230662589825E-4	0.0	0.0	16.561664831060003	0.0
23	3.8509230662589825E-4	0.0	0.0	17.11119155261516	0.0
24	3.8509230662589825E-4	0.0	0.0	17.53614091297684	0.0
25	3.8509230662589825E-4	0.0	0.0	17.906792258104268	0.0
26	3.8509230662589825E-4	0.0	0.0	18.289381464737097	0.0
27	3.8509230662589825E-4	0.0	0.0	18.706628978966258	0.0
28	3.8509230662589825E-4	0.0	0.0	19.08093870100663	0.0
29	3.8509230662589825E-4	0.0	0.0	19.498378761389105	0.0
30	3.8509230662589825E-4	0.0	0.0	19.965303183173006	0.0
31	3.8509230662589825E-4	0.0	0.0	20.384861251241922	0.0
32	3.8509230662589825E-4	0.0	0.0	20.790555996272307	0.0
33	3.8509230662589825E-4	0.0	0.0	21.18951162593674	0.0
34	3.8509230662589825E-4	0.0	0.0	21.610224970925533	0.0
35	3.8509230662589825E-4	0.0	0.0	22.03979543896672	0.0
36	3.8509230662589825E-4	0.0	0.0	22.442794537850723	0.0
37	3.8509230662589825E-4	0.0	0.0	22.84348308289497	0.0
38	3.8509230662589825E-4	0.0	0.0	23.245519451012406	0.0
39	3.8509230662589825E-4	0.0	0.0	23.690108519012007	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	30	2.163195E-6	45.000004	20
GCGATAT	30	2.163195E-6	45.000004	9
CGACAAA	35	1.21021E-7	45.0	12
TAGCACG	20	7.029712E-4	45.0	1
ACGATAG	20	7.029712E-4	45.0	1
CCCGTAA	20	7.029712E-4	45.0	41
ACGGCCA	35	1.21021E-7	45.0	30
AGCGCGA	20	7.029712E-4	45.0	9
CGATGCG	25	3.887842E-5	45.0	10
CGCCCAC	20	7.029712E-4	45.0	34
GACTACG	25	3.887842E-5	45.0	1
CTCGAAT	90	0.0	42.5	43
CGTTTTT	1845	0.0	42.19512	1
TACGGGA	290	0.0	41.896553	4
CCGATGA	905	0.0	41.767956	18
CGATGAA	960	0.0	41.250004	19
CGAATAT	50	1.0786607E-9	40.5	14
GCTACGA	50	1.0786607E-9	40.5	10
ATTGCGG	90	0.0	40.0	2
CCACCGA	1030	0.0	39.975727	15
>>END_MODULE