FastQCFastQC Report
Sat 18 Jun 2016
SRR3554436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356063
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50991.432049946217383No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG29900.839739034946063No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC29900.839739034946063No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC29510.8287859170989403No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC12140.3409508991386356No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT8560.24040689428556183No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT7670.215411317660077No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT6460.18142856741644034No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT5250.1474458171728037No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC4930.13845864355465184No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA4830.1356501517989794No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC4600.12919062076093274No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC4530.12722467653196204No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG4330.12160769302061714No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC207.02672E-445.0000049
CGTGAAC207.02672E-445.00000442
GGCCGGT207.02672E-445.0000049
CACGCGT207.02672E-445.00000441
CCTCGTC406.7921064E-945.00000436
CCCACGC207.02672E-445.00000439
TATTGCG207.02672E-445.0000041
AGCGGAA207.02672E-445.0000044
CCGACTT207.02672E-445.00000444
ACCACTC207.02672E-445.00000423
ACCCGTG406.7921064E-945.00000439
ACCCGTC207.02672E-445.00000439
CAGTCGA207.02672E-445.00000441
GTAACGG406.7921064E-945.0000042
AGTAACG207.02672E-445.0000041
ACGAAAC207.02672E-445.00000436
CGGCGAA502.1827873E-1145.031
GTCGACG253.8853646E-545.01
GATCGAG253.8853646E-545.01
GCGAACA253.8853646E-545.033