##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356063
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.856736027051394	33.0	31.0	34.0	30.0	34.0
2	31.97884924858803	33.0	31.0	34.0	30.0	34.0
3	31.96335199108023	33.0	31.0	34.0	30.0	34.0
4	35.64412758416348	37.0	35.0	37.0	33.0	37.0
5	35.44740116215389	37.0	35.0	37.0	33.0	37.0
6	35.595057616208365	37.0	35.0	37.0	33.0	37.0
7	35.95559774534282	37.0	35.0	37.0	35.0	37.0
8	35.832526828117494	37.0	35.0	37.0	35.0	37.0
9	37.700842266677526	39.0	38.0	39.0	35.0	39.0
10	37.21124913287817	39.0	37.0	39.0	34.0	39.0
11	37.19328882810065	39.0	37.0	39.0	34.0	39.0
12	37.22395755807259	39.0	37.0	39.0	34.0	39.0
13	37.249177814038525	39.0	37.0	39.0	34.0	39.0
14	38.459921418400675	40.0	38.0	41.0	34.0	41.0
15	38.528035768951	40.0	38.0	41.0	34.0	41.0
16	38.5048909883925	40.0	38.0	41.0	34.0	41.0
17	38.4590142755636	40.0	38.0	41.0	34.0	41.0
18	38.3302140351567	40.0	38.0	41.0	34.0	41.0
19	38.09500285061913	40.0	37.0	41.0	34.0	41.0
20	37.908367339487675	40.0	35.0	41.0	34.0	41.0
21	37.91555707838219	40.0	35.0	41.0	34.0	41.0
22	37.97686926190028	40.0	36.0	41.0	34.0	41.0
23	37.897933792615355	40.0	35.0	41.0	34.0	41.0
24	37.87024206390442	40.0	35.0	41.0	34.0	41.0
25	37.726346180310784	40.0	35.0	41.0	33.0	41.0
26	37.74858662652396	40.0	35.0	41.0	33.0	41.0
27	37.683853700047464	40.0	35.0	41.0	33.0	41.0
28	37.584435900388414	40.0	35.0	41.0	33.0	41.0
29	37.537845830653566	40.0	35.0	41.0	33.0	41.0
30	37.305858794651506	40.0	35.0	41.0	33.0	41.0
31	37.143053336066934	40.0	35.0	41.0	32.0	41.0
32	36.77250935929877	40.0	35.0	41.0	31.0	41.0
33	36.46845923333792	40.0	35.0	41.0	30.0	41.0
34	36.140025781954314	40.0	35.0	41.0	27.0	41.0
35	35.89288412443865	40.0	35.0	41.0	24.0	41.0
36	35.69667446491211	40.0	35.0	41.0	23.0	41.0
37	35.60498563456467	40.0	35.0	41.0	23.0	41.0
38	35.55676102262802	39.0	35.0	41.0	23.0	41.0
39	35.51732418139486	39.0	35.0	41.0	23.0	41.0
40	35.459238954904045	39.0	35.0	41.0	23.0	41.0
41	35.4054956566675	39.0	35.0	41.0	22.0	41.0
42	35.39041405593954	39.0	35.0	41.0	22.0	41.0
43	35.29110859595072	39.0	35.0	41.0	21.0	41.0
44	35.303892850422535	39.0	35.0	41.0	21.0	41.0
45	35.28993183790509	39.0	35.0	41.0	22.0	41.0
46	35.15885391068434	39.0	35.0	41.0	21.0	41.0
47	35.04907839343038	39.0	35.0	41.0	20.0	41.0
48	35.001348076042724	39.0	35.0	41.0	20.0	41.0
49	35.0059174921292	39.0	35.0	41.0	20.0	41.0
50	34.899728418847225	39.0	35.0	41.0	20.0	41.0
51	34.127519568166306	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	5.0
12	6.0
13	7.0
14	12.0
15	13.0
16	32.0
17	74.0
18	119.0
19	284.0
20	564.0
21	1118.0
22	1654.0
23	2347.0
24	3310.0
25	5186.0
26	7317.0
27	7596.0
28	6442.0
29	5804.0
30	5838.0
31	6445.0
32	7731.0
33	9884.0
34	16557.0
35	22529.0
36	22698.0
37	32876.0
38	62524.0
39	126963.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37441969539098	18.466114142721935	22.44855545226547	17.71091070962161
2	37.250430401361555	25.875477092536997	22.951837174881973	13.922255331219475
3	27.73554118231886	25.12420554789461	32.731286317309014	14.408966952477511
4	23.769389124958224	22.65160940620059	37.43944189651831	16.139559572322877
5	25.89625993152897	26.560749080921077	31.269185509305935	16.273805478244018
6	21.89022729123779	34.59809078730449	30.534203216846457	12.977478704611261
7	72.31444997093212	4.418319230023901	19.488405141786703	3.7788256572572827
8	73.702687445761	7.374537652044723	15.29925883902568	3.623516063168596
9	68.63335982677224	6.255072838233683	16.445404324515607	8.666163010478483
10	34.15912352589289	29.220671622718452	24.29598132914681	12.324223522241851
11	24.64142581509452	23.051257783032778	35.23365247161317	17.073663930259535
12	21.3302140351567	21.188946899846375	37.12601421658527	20.354824848411656
13	21.618084440113126	22.63194996391088	39.69185228456762	16.058113311408377
14	18.081350772194806	28.38963891221497	35.35694525968719	18.172065055903026
15	16.42546403305033	25.5230113772001	40.06678593394989	17.984738655799674
16	19.094092899290295	25.0857292108419	35.71924069616894	20.10093719369887
17	19.459758525878847	25.573845077977776	35.89252463749393	19.073871758649453
18	19.393478120444975	24.341198046413133	36.3309302005544	19.93439363258749
19	18.960408691720286	26.671403656094565	34.18552334839621	20.182664303788936
20	21.176870385296983	25.699665508631895	36.50196734847485	16.62149675759627
21	19.533060160701897	28.01976054799291	35.581343750965424	16.86583554033977
22	18.63687044146682	22.99508794791933	37.120116383898356	21.2479252267155
23	18.455161024874812	26.587429752599963	35.60858612099544	19.348823101529785
24	18.673942532641696	25.957204202627064	35.250503422147204	20.11834984258404
25	17.66316634977518	29.42372557665357	33.86479358989842	19.048314483672833
26	18.532113698980236	25.942600045497567	35.807989035648184	19.717297219874013
27	20.393020336288803	26.50682603921216	34.28157376644021	18.818579858058825
28	17.62862190118041	26.71212678655182	36.54999255749685	19.109258754770924
29	20.43402431592162	23.929192305855988	35.909094738852396	19.72768863937
30	19.24266211316537	26.479302820006573	36.06243838871211	18.21559667811595
31	20.87636176744003	25.27895344363217	32.58945748364756	21.255227305280243
32	21.112555924092085	28.016952056237237	32.30242962621783	18.568062393452845
33	20.737341425534243	25.670176345197337	32.75235000547656	20.84013222379186
34	20.99796946046065	25.949059576535618	31.645523404566045	21.407447558437692
35	20.04027377177634	26.71016084232285	32.40915231293339	20.840413072967422
36	23.488820798566547	27.031452299171775	30.38731909802479	19.092407804236892
37	19.92652985567161	28.268873766721057	32.4313393978032	19.373256979804136
38	20.516313124362824	27.42436029579035	30.952106790090518	21.107219789756307
39	20.906412629225727	27.442896341377793	30.533079820144188	21.117611209252296
40	19.87962804335188	26.60484240148513	32.62203598801336	20.893493567149633
41	20.25765103366539	26.878951196838763	29.910156348736038	22.95324142075981
42	21.273763350867682	27.178617267169013	31.5250391082477	20.022580273715608
43	22.878535540058923	25.764822517363502	30.568747665441226	20.78789427713635
44	20.945450664629575	25.636193594953703	31.780050159662753	21.638305580753965
45	20.227038473528562	25.037704001819904	31.033833900180586	23.701423624470948
46	22.05003047213555	27.3356119563111	29.962675144567115	20.651682426986234
47	19.243785509867635	26.30742312455942	34.12542162482482	20.323369740748127
48	20.385718257724054	26.169807028531466	31.478979843454667	21.96549487028981
49	20.555912858117807	24.53301803332556	33.37218413595347	21.53888497260316
50	20.57472975288081	24.34035549888643	32.20525581147157	22.879658936761192
51	19.427460870688616	24.15106315455411	31.054055040821428	25.367420933935847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	274.0
2	413.0
3	4516.5
4	8620.0
5	5978.5
6	3337.0
7	3321.5
8	3306.0
9	3320.0
10	3334.0
11	3256.5
12	3179.0
13	3039.5
14	2900.0
15	2782.5
16	2665.0
17	2577.5
18	2490.0
19	2533.5
20	2577.0
21	2541.5
22	2506.0
23	2571.5
24	2637.0
25	2935.5
26	3666.5
27	4099.0
28	4783.0
29	5467.0
30	6026.0
31	6585.0
32	7587.5
33	8590.0
34	9805.0
35	11020.0
36	12048.5
37	13077.0
38	14127.5
39	15178.0
40	16274.0
41	17370.0
42	19278.0
43	21186.0
44	22497.5
45	23809.0
46	32723.5
47	41638.0
48	37727.0
49	33816.0
50	32797.0
51	31778.0
52	27483.0
53	23188.0
54	20337.5
55	17487.0
56	15052.5
57	12618.0
58	10897.5
59	9177.0
60	8025.0
61	6873.0
62	5934.5
63	4996.0
64	3977.5
65	2959.0
66	2321.0
67	1683.0
68	1308.0
69	933.0
70	715.0
71	497.0
72	415.5
73	334.0
74	270.5
75	154.5
76	102.0
77	70.0
78	38.0
79	28.0
80	18.0
81	11.0
82	4.0
83	3.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	356063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0828449296175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29882006804229	34.39767873440338
2	8.567384405032001	7.72484125168962
3	3.504561017145412	4.739867426470474
4	2.226169081057374	4.0144814187367475
5	1.6031946633740684	3.6138288200441724
6	1.2610953186096505	3.411225881420727
7	1.0765427436394808	3.3973526700122574
8	0.8671196042559328	3.1273774923281215
9	0.7382131646829697	2.9952674665564056
>10	3.802039945303679	24.71709644714023
>50	0.03241726614286496	1.026115363255754
>100	0.016832042035718343	1.7652781694356041
>500	0.0024936358571434583	0.7852550876298485
>1k	0.0024936358571434583	2.851257288477026
>5k	6.234089642858646E-4	1.4330764823996411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5099	1.432049946217383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	2990	0.839739034946063	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	2990	0.839739034946063	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	2951	0.8287859170989403	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	1214	0.3409508991386356	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT	856	0.24040689428556183	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	767	0.215411317660077	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	646	0.18142856741644034	No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT	525	0.1474458171728037	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC	493	0.13845864355465184	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA	483	0.1356501517989794	No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	460	0.12919062076093274	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	453	0.12722467653196204	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	433	0.12160769302061714	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15222025315744686	0.0
2	0.0	0.0	0.0	0.7709309869320878	0.0
3	0.0	0.0	0.0	1.0913798962543146	0.0
4	0.0	0.0	0.0	1.646337867175191	0.0
5	0.0	0.0	0.0	3.3407009433723807	0.0
6	0.0	0.0	0.0	4.147861473952643	0.0
7	0.0	0.0	0.0	4.973838899295911	0.0
8	0.0	0.0	0.0	6.022810570039572	0.0
9	0.0	0.0	0.0	6.514015778106684	0.0
10	0.0	0.0	0.0	8.309203708332515	0.0
11	0.0	0.0	0.0	9.51151902893589	0.0
12	0.0	0.0	0.0	11.282834779238506	0.0
13	0.0	0.0	0.0	11.777691026587991	0.0
14	0.0	0.0	0.0	12.011076691484373	0.0
15	0.0	0.0	0.0	12.43122705813297	0.0
16	0.0	0.0	0.0	13.026627310335531	0.0
17	0.0	0.0	0.0	13.797558297267619	0.0
18	0.0	0.0	0.0	14.599944391863238	0.0
19	0.0	0.0	0.0	15.3908156702606	0.0
20	0.0	0.0	0.0	15.923305707136096	0.0
21	0.0	0.0	0.0	16.516178316758552	0.0
22	0.0	0.0	0.0	17.201731154318196	0.0
23	2.808491755672451E-4	0.0	0.0	17.87913936578639	0.0
24	2.808491755672451E-4	0.0	0.0	18.382701937578464	0.0
25	2.808491755672451E-4	0.0	0.0	18.860426385218346	0.0
26	2.808491755672451E-4	0.0	0.0	19.31175101035491	0.0
27	2.808491755672451E-4	0.0	0.0	19.784139323659016	0.0
28	2.808491755672451E-4	0.0	0.0	20.26495311223014	0.0
29	2.808491755672451E-4	0.0	0.0	20.76009020875519	0.0
30	2.808491755672451E-4	0.0	0.0	21.30662270440905	0.0
31	2.808491755672451E-4	0.0	0.0	21.82984471849083	0.0
32	2.808491755672451E-4	0.0	0.0	22.3342498378096	0.0
33	2.808491755672451E-4	0.0	0.0	22.79006804975524	0.0
34	2.808491755672451E-4	0.0	0.0	23.221733232602094	0.0
35	2.808491755672451E-4	0.0	0.0	23.711253345615805	0.0
36	2.808491755672451E-4	0.0	0.0	24.21341167153004	0.0
37	2.808491755672451E-4	0.0	0.0	24.678778755444963	0.0
38	2.808491755672451E-4	0.0	0.0	25.152009616275773	0.0
39	2.808491755672451E-4	0.0	0.0	25.644338221045153	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.02672E-4	45.000004	9
CGTGAAC	20	7.02672E-4	45.000004	42
GGCCGGT	20	7.02672E-4	45.000004	9
CACGCGT	20	7.02672E-4	45.000004	41
CCTCGTC	40	6.7921064E-9	45.000004	36
CCCACGC	20	7.02672E-4	45.000004	39
TATTGCG	20	7.02672E-4	45.000004	1
AGCGGAA	20	7.02672E-4	45.000004	4
CCGACTT	20	7.02672E-4	45.000004	44
ACCACTC	20	7.02672E-4	45.000004	23
ACCCGTG	40	6.7921064E-9	45.000004	39
ACCCGTC	20	7.02672E-4	45.000004	39
CAGTCGA	20	7.02672E-4	45.000004	41
GTAACGG	40	6.7921064E-9	45.000004	2
AGTAACG	20	7.02672E-4	45.000004	1
ACGAAAC	20	7.02672E-4	45.000004	36
CGGCGAA	50	2.1827873E-11	45.0	31
GTCGACG	25	3.8853646E-5	45.0	1
GATCGAG	25	3.8853646E-5	45.0	1
GCGAACA	25	3.8853646E-5	45.0	33
>>END_MODULE