FastQCFastQC Report
Sat 18 Jun 2016
SRR3554435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946558
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360233.805683328438405No Hit
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG116701.2328880005240037TruSeq Adapter, Index 27 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC100041.056881881511751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91090.9623287743593103No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT85870.9071815990145348No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC77310.8167486831234854No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT75640.7991058128503483No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG64950.6861703139163158No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC58990.6232053397678748No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT55100.5821090730837414No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.5006560612239292No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC31650.3343693677513686TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC28060.29644247896061304TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC26010.2747850633558641No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC25290.2671785564117571No Hit
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT24650.26041721690588426No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT20960.2214338688173361TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGT19800.2091789409629415No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG17740.18741587942841326No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15920.16818832020858734No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT14700.15529951677551718No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT14510.15329224410971118No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTA13350.14103731625531662No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC12670.13385339303032673No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC12500.13205741222407924No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT10850.11462583381050077No Hit
GAATATGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC10610.11209033149579845No Hit
CGCTATCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG10030.10596286756860118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGTA207.032654E-445.00000437
TCCCGAT207.032654E-445.00000432
TCGACGG406.8121153E-945.0000042
GTCGCGT253.890283E-545.019
GTTTACG253.890283E-545.01
AATGTCG253.890283E-545.016
GTACGAG351.2116288E-745.01
CGGTCTA1300.044.99999631
CGTTTTT123100.044.3419951
CGTTATT17450.043.9684831
CGTAAGG1150.043.043482
CGTTTCT9000.042.251
TACGGCT10550.042.2274867
CGTTCTG7200.041.56251
TAATACG2350.041.170214
TCGTTTA556.002665E-1140.90909238
CTAACGG501.0804797E-940.52
GTTATTT18850.040.464192
TGATACC8400.040.446434
ACGGTCT1450.040.34482630
Produced by FastQC (version 0.11.5)