##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554435_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 946558 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.043029587199094 33.0 31.0 34.0 30.0 34.0 2 32.20836124146645 33.0 31.0 34.0 30.0 34.0 3 32.07096659686992 33.0 31.0 34.0 30.0 34.0 4 35.783398376010766 37.0 35.0 37.0 35.0 37.0 5 31.699554596760052 37.0 35.0 37.0 0.0 37.0 6 33.649074858592925 37.0 35.0 37.0 17.0 37.0 7 35.43073218968093 37.0 35.0 37.0 32.0 37.0 8 35.656733132042625 37.0 35.0 37.0 33.0 37.0 9 37.72216493865141 39.0 37.0 39.0 35.0 39.0 10 37.50130050139558 39.0 37.0 39.0 35.0 39.0 11 37.44982874794783 39.0 37.0 39.0 35.0 39.0 12 37.46627253691797 39.0 37.0 39.0 35.0 39.0 13 37.42113003112329 39.0 37.0 39.0 35.0 39.0 14 38.68563574551163 40.0 38.0 41.0 35.0 41.0 15 38.705898634843294 40.0 38.0 41.0 35.0 41.0 16 38.68611537803283 40.0 38.0 41.0 35.0 41.0 17 38.60134085814076 40.0 38.0 41.0 34.0 41.0 18 38.365691273012324 40.0 38.0 41.0 34.0 41.0 19 38.111832555427135 40.0 37.0 41.0 34.0 41.0 20 37.853982534614886 39.0 35.0 41.0 34.0 41.0 21 37.762643176646336 39.0 35.0 41.0 34.0 41.0 22 37.762297714455954 39.0 35.0 41.0 34.0 41.0 23 37.6881406105067 39.0 35.0 41.0 34.0 41.0 24 37.63407841886076 39.0 35.0 41.0 33.0 41.0 25 37.41125636252612 39.0 35.0 41.0 33.0 41.0 26 37.4675033119999 39.0 35.0 41.0 33.0 41.0 27 37.423516572677 39.0 35.0 41.0 33.0 41.0 28 37.35179143803127 39.0 35.0 41.0 33.0 41.0 29 37.312454176077956 39.0 35.0 41.0 33.0 41.0 30 37.0598283464933 39.0 35.0 41.0 33.0 41.0 31 36.91845613264058 39.0 35.0 41.0 32.0 41.0 32 36.57737296605174 39.0 35.0 41.0 31.0 41.0 33 36.18769478468303 39.0 35.0 41.0 30.0 41.0 34 35.834820475871524 39.0 35.0 41.0 27.0 41.0 35 35.63352589064801 39.0 35.0 41.0 25.0 41.0 36 35.51276519769523 39.0 35.0 41.0 24.0 41.0 37 35.46313168342563 39.0 35.0 41.0 23.0 41.0 38 35.26940240323361 39.0 35.0 41.0 23.0 41.0 39 35.215547277610035 39.0 35.0 41.0 23.0 41.0 40 35.129226101305996 39.0 35.0 41.0 22.0 41.0 41 35.031873377014406 39.0 35.0 41.0 22.0 41.0 42 34.95062109242117 39.0 35.0 41.0 21.0 41.0 43 34.9475932800737 39.0 35.0 41.0 21.0 41.0 44 34.9165798609277 39.0 35.0 41.0 21.0 41.0 45 34.9053771665339 39.0 35.0 41.0 20.0 41.0 46 34.810911745503184 39.0 35.0 41.0 20.0 41.0 47 34.70983605864617 38.0 35.0 41.0 20.0 41.0 48 34.671731684693384 38.0 35.0 40.0 20.0 41.0 49 34.64013193063711 38.0 35.0 40.0 20.0 41.0 50 34.52347029975976 38.0 34.0 40.0 20.0 41.0 51 33.71748376750289 37.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 9.0 10 9.0 11 17.0 12 25.0 13 26.0 14 48.0 15 53.0 16 74.0 17 176.0 18 325.0 19 683.0 20 1249.0 21 1963.0 22 3145.0 23 4760.0 24 7479.0 25 12925.0 26 18755.0 27 21202.0 28 19664.0 29 17786.0 30 17451.0 31 19759.0 32 24158.0 33 32730.0 34 50492.0 35 73826.0 36 79138.0 37 101885.0 38 168620.0 39 267910.0 40 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.365230656758484 17.719040988507835 17.687452855503835 28.228275499229845 2 42.724587399821246 25.041360381508582 19.19713319204951 13.03691902662066 3 24.256728061037993 24.966246125435525 37.47493550315987 13.302090310366612 4 21.928397414632805 22.33978266519326 40.65952641042598 15.07229350974795 5 20.779709220142877 33.82550250486499 30.687501452631533 14.707286822360595 6 22.228326209276137 31.62901797882433 34.31929158065327 11.823364231246263 7 65.90520602012766 4.50051660859662 24.590991782859582 5.003285588416135 8 64.68457294745805 7.330559775523528 22.56480849562309 5.42005878139533 9 58.85291762364271 7.322108101141188 23.668808461816393 10.156165813399708 10 25.89392303482724 32.05403155432631 28.699033762326238 13.35301164852022 11 23.20629058124278 20.1539683780603 38.84579708797559 17.79394395272133 12 21.79898115065321 19.408319405678256 38.66683288292952 20.125866560739013 13 19.322957494416613 21.25374250706243 42.55037726161524 16.872922736905714 14 16.865527521821168 24.777245557060425 38.10976189520346 20.247465025914945 15 14.2793151608248 25.1997236302477 41.51451892012956 19.00644228879794 16 16.490378825175004 25.76313337376051 38.09983117780421 19.646656623260274 17 16.343742274641386 26.427223688352957 36.826480786174756 20.402553250830906 18 15.33197120514538 26.106376999613335 37.327876368907134 21.23377542633415 19 15.424728331491572 28.16362019020493 35.62095508146358 20.79069639683992 20 16.805626279636325 26.56171095696196 37.43711426029889 19.19554850310282 21 17.393968462577043 27.863374457772267 36.98135771923115 17.761299360419542 22 17.817080411342992 23.483399855053786 36.91226528115551 21.78725445244771 23 16.679379393550104 26.77722865371166 36.868844804016234 19.674547148722002 24 18.158316764530014 23.467024735938 36.739322894106856 21.63533560542513 25 18.087533991577907 27.397158969656378 34.139376562239185 20.37593047652653 26 18.027949687182403 24.896625457710993 34.627460757819385 22.447964097287223 27 19.71088934856607 24.519997718047918 33.231878025435314 22.5372349079507 28 18.90301492354404 24.17390165209105 34.93013634663698 21.992947077727937 29 20.547710758347613 21.90050688917108 33.38210653758142 24.16967581489988 30 21.49197407871467 24.80481914473281 33.82603073451389 19.877176042038627 31 22.26899989224115 23.852209795913193 32.7285808159669 21.15020949587875 32 20.881868834239423 27.026869985780056 33.13753621014243 18.95372496983809 33 19.365110220398538 25.71960725069145 33.95449618512548 20.960786343784534 34 18.522583930408913 25.313398650690182 34.31918593472349 21.84483148417741 35 18.374257044998828 25.966818726374928 33.536455240988936 22.122468987637316 36 18.765675214831 26.04563058999026 31.87897624868207 23.309717946496676 37 19.516923421491338 26.590341003932146 33.291145392041486 20.601590182535038 38 18.666684978627828 26.238540057767196 33.62223973596969 21.472535227635284 39 18.59188766034411 26.55928110057704 33.61949294179543 21.22933829728342 40 19.50561930700496 25.555222183954918 35.87038512167241 19.068773387367706 41 18.282978961669542 25.746335670925607 34.38024928213591 21.590436085268944 42 18.49469340494719 26.700952292411028 33.17979458205414 21.624559720587644 43 19.730328199645452 24.77502699253506 32.00606830220652 23.48857650561297 44 20.772419650988105 24.470344131051665 31.76561816602892 22.991618051931315 45 19.880451065861784 22.99806245364785 32.23743288842311 24.88405359206726 46 20.9568774443827 24.64381474774921 30.94041780852309 23.458889999344994 47 17.9999535157909 23.478962726003054 36.3870993642228 22.13398439398325 48 18.569490723230906 23.661201954872286 34.76057462934126 23.008732692555554 49 18.222760781695364 22.627350886052415 35.701562925885156 23.44832540636707 50 18.174480591786242 21.623608907219634 36.54081419205163 23.661096308942504 51 18.166979730771914 21.245607770469427 35.031873377014406 25.555539121744257 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10611.0 1 7900.0 2 5189.0 3 29902.0 4 54615.0 5 33380.5 6 12146.0 7 11444.5 8 10743.0 9 10592.0 10 10441.0 11 10419.0 12 10397.0 13 9940.5 14 9484.0 15 8926.5 16 8369.0 17 7841.5 18 7314.0 19 6697.0 20 6080.0 21 5791.5 22 5503.0 23 5347.5 24 5192.0 25 5369.5 26 5999.0 27 6451.0 28 7350.5 29 8250.0 30 9939.0 31 11628.0 32 13051.0 33 14474.0 34 17136.0 35 19798.0 36 21461.5 37 23125.0 38 25325.5 39 27526.0 40 30494.5 41 33463.0 42 36890.5 43 40318.0 44 48470.5 45 56623.0 46 84978.0 47 113333.0 48 105477.0 49 97621.0 50 93739.0 51 89857.0 52 72672.0 53 55487.0 54 49127.0 55 42767.0 56 37646.5 57 32526.0 58 30357.5 59 28189.0 60 26314.5 61 24440.0 62 21636.0 63 18832.0 64 16044.5 65 13257.0 66 11447.5 67 9638.0 68 7766.5 69 5895.0 70 4788.5 71 3682.0 72 3277.0 73 2872.0 74 2353.0 75 1573.5 76 1313.0 77 1083.5 78 854.0 79 680.0 80 506.0 81 371.0 82 236.0 83 142.5 84 49.0 85 47.0 86 45.0 87 25.5 88 6.0 89 18.5 90 31.0 91 16.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 946558.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.33761431416435 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37389346091057 26.224972973318255 2 9.414417057502247 6.465372438264041 3 3.6460747007380494 3.7559252050372605 4 1.9899252577206954 2.7331714405450933 5 1.2226613423781258 2.0991636805709266 6 0.8485346473309072 1.748199327135249 7 0.6196434781877558 1.489395513640893 8 0.5277046164664918 1.4496094073624335 9 0.42519691066444315 1.3140222773372858 >10 4.8087135642558625 32.81549711260002 >50 0.07640570446246966 1.7614015699406196 >100 0.036354293467847916 2.388099074832053 >500 0.0018485233966671108 0.49382681320571203 >1k 0.005545570190001332 3.7734165810271088 >5k 0.0021566106294449625 5.384172163261507 >10k+ 9.242616983335554E-4 6.103754421921588 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36023 3.805683328438405 No Hit CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 11670 1.2328880005240037 TruSeq Adapter, Index 27 (95% over 21bp) GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC 10004 1.056881881511751 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9109 0.9623287743593103 No Hit CGTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT 8587 0.9071815990145348 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC 7731 0.8167486831234854 No Hit CGTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT 7564 0.7991058128503483 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG 6495 0.6861703139163158 No Hit CGTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC 5899 0.6232053397678748 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT 5510 0.5821090730837414 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4739 0.5006560612239292 No Hit CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 3165 0.3343693677513686 TruSeq Adapter, Index 20 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 2806 0.29644247896061304 TruSeq Adapter, Index 20 (95% over 21bp) CGTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC 2601 0.2747850633558641 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC 2529 0.2671785564117571 No Hit GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT 2465 0.26041721690588426 No Hit CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT 2096 0.2214338688173361 TruSeq Adapter, Index 27 (95% over 23bp) CGTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGT 1980 0.2091789409629415 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG 1774 0.18741587942841326 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1592 0.16818832020858734 No Hit GAATGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT 1470 0.15529951677551718 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT 1451 0.15329224410971118 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTA 1335 0.14103731625531662 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC 1267 0.13385339303032673 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC 1250 0.13205741222407924 No Hit GAATGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT 1085 0.11462583381050077 No Hit GAATATGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC 1061 0.11209033149579845 No Hit CGCTATCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 1003 0.10596286756860118 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.22808956239342967 0.0 2 0.0 0.0 0.0 0.9564126022916715 0.0 3 0.0 0.0 0.0 2.3879149508006905 0.0 4 0.0 0.0 0.0 3.879318541494552 0.0 5 0.0 0.0 0.0 6.4882447773934615 0.0 6 0.0 0.0 0.0 8.822914179585403 0.0 7 0.0 0.0 0.0 10.656399290904519 0.0 8 0.0 0.0 0.0 12.532671003784237 0.0 9 0.0 0.0 0.0 13.535990398897901 0.0 10 0.0 0.0 0.0 15.56851244192115 0.0 11 0.0 0.0 0.0 16.971596035319546 0.0 12 0.0 0.0 0.0 19.003801140553456 0.0 13 0.0 0.0 0.0 19.66746887142679 0.0 14 0.0 0.0 0.0 20.07050809353468 0.0 15 0.0 0.0 0.0 20.602963579622166 0.0 16 0.0 0.0 0.0 21.16077408885668 0.0 17 0.0 0.0 0.0 21.77605598389111 0.0 18 0.0 0.0 0.0 22.371582090056815 0.0 19 0.0 0.0 0.0 22.97175661713281 0.0 20 0.0 0.0 0.0 23.43649306223179 0.0 21 0.0 0.0 0.0 23.939367687981086 0.0 22 0.0 0.0 0.0 24.505841163457497 0.0 23 0.0 0.0 0.0 25.0733710982317 0.0 24 0.0 0.0 0.0 25.493947544682946 0.0 25 0.0 0.0 0.0 25.91811595274669 0.0 26 0.0 0.0 0.0 26.30393488830056 0.0 27 0.0 0.0 0.0 26.698733727885667 0.0 28 0.0 0.0 0.0 27.090046251788056 0.0 29 0.0 0.0 0.0 27.48812011519632 0.0 30 0.0 0.0 0.0 27.94440488591296 0.0 31 0.0 0.0 0.0 28.36614343759178 0.0 32 0.0 0.0 0.0 28.7822827549923 0.0 33 0.0 0.0 0.0 29.17803240794542 0.0 34 0.0 0.0 0.0 29.561632778973923 0.0 35 0.0 0.0 0.0 29.99657707187515 0.0 36 0.0 0.0 0.0 30.404581652682666 0.0 37 0.0 0.0 0.0 30.815438673594223 0.0 38 0.0 0.0 0.0 31.219534354999904 0.0 39 0.0 0.0 0.0 31.63039137591146 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGTA 20 7.032654E-4 45.000004 37 TCCCGAT 20 7.032654E-4 45.000004 32 TCGACGG 40 6.8121153E-9 45.000004 2 GTCGCGT 25 3.890283E-5 45.0 19 GTTTACG 25 3.890283E-5 45.0 1 AATGTCG 25 3.890283E-5 45.0 16 GTACGAG 35 1.2116288E-7 45.0 1 CGGTCTA 130 0.0 44.999996 31 CGTTTTT 12310 0.0 44.341995 1 CGTTATT 1745 0.0 43.968483 1 CGTAAGG 115 0.0 43.04348 2 CGTTTCT 900 0.0 42.25 1 TACGGCT 1055 0.0 42.227486 7 CGTTCTG 720 0.0 41.5625 1 TAATACG 235 0.0 41.17021 4 TCGTTTA 55 6.002665E-11 40.909092 38 CTAACGG 50 1.0804797E-9 40.5 2 GTTATTT 1885 0.0 40.46419 2 TGATACC 840 0.0 40.44643 4 ACGGTCT 145 0.0 40.344826 30 >>END_MODULE