SRR3554434_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1065588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5898	0.5534972240678386	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	2930	0.2749655589214593	No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2929	0.2748717140208035	RNA PCR Primer, Index 13 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	2727	0.2559150440883343	RNA PCR Primer, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2507	0.23526916594406094	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	2477	0.2324538189243873	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2349	0.22044167164044637	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	1955	0.18346678078206585	RNA PCR Primer, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1748	0.16404088634631772	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	1249	0.11721228091907943	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	65	0.0	45.000004	4
CGAATAA	35	1.2118107E-7	45.000004	23
CGATATA	35	1.2118107E-7	45.000004	12
TAGACCG	20	7.033055E-4	45.0	1
CGTCTAT	20	7.033055E-4	45.0	13
ATCCGCG	20	7.033055E-4	45.0	1
GCCGATT	50	2.1827873E-11	45.0	9
TCGTCGT	25	3.890614E-5	45.0	42
GTCAACG	20	7.033055E-4	45.0	1
CCGCGTA	20	7.033055E-4	45.0	33
AATAGCG	45	3.8562575E-10	45.0	1
CGGCCTA	60	3.6379788E-12	41.249996	45
CTCGAAC	60	3.6379788E-12	41.249996	13
GTAAGCG	60	3.6379788E-12	41.249996	1
ACTACGG	55	6.184564E-11	40.909092	2
CGTTTTT	2555	0.0	40.861053	1
CGTATGG	90	0.0	40.0	2
GCGTATG	45	1.9281288E-8	40.0	1
CGTTATT	440	0.0	39.886364	1
TAAGCGG	130	0.0	39.807693	2