##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1065588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.195377575573296	33.0	31.0	34.0	30.0	34.0
2	32.295484746449844	34.0	31.0	34.0	30.0	34.0
3	32.26550974673138	34.0	31.0	34.0	30.0	34.0
4	35.87626831383236	37.0	35.0	37.0	35.0	37.0
5	31.818392286699925	37.0	35.0	37.0	0.0	37.0
6	33.761326141060145	37.0	35.0	37.0	17.0	37.0
7	35.56944053424025	37.0	35.0	37.0	32.0	37.0
8	35.831530572791735	37.0	35.0	37.0	35.0	37.0
9	37.854832261624566	39.0	38.0	39.0	35.0	39.0
10	37.49007871710267	39.0	37.0	39.0	35.0	39.0
11	37.40306384831661	39.0	37.0	39.0	35.0	39.0
12	37.40129487193925	39.0	37.0	39.0	35.0	39.0
13	37.34959665461698	39.0	37.0	39.0	35.0	39.0
14	38.55439719666513	40.0	38.0	41.0	34.0	41.0
15	38.66399114854897	40.0	38.0	41.0	35.0	41.0
16	38.63501090477746	40.0	38.0	41.0	35.0	41.0
17	38.593261185373706	40.0	38.0	41.0	35.0	41.0
18	38.522210272638205	40.0	38.0	41.0	34.0	41.0
19	38.47744062433136	40.0	38.0	41.0	34.0	41.0
20	38.46145977619868	40.0	38.0	41.0	34.0	41.0
21	38.35756502513166	40.0	37.0	41.0	34.0	41.0
22	38.40199401644914	40.0	37.0	41.0	34.0	41.0
23	38.35866019512232	40.0	37.0	41.0	34.0	41.0
24	38.33465373108556	40.0	37.0	41.0	34.0	41.0
25	38.130165692556595	40.0	37.0	41.0	34.0	41.0
26	38.230608828177495	40.0	37.0	41.0	34.0	41.0
27	38.2046222367369	40.0	37.0	41.0	34.0	41.0
28	38.18996272480546	40.0	37.0	41.0	34.0	41.0
29	38.18988671043593	40.0	37.0	41.0	34.0	41.0
30	38.026331002226	40.0	37.0	41.0	34.0	41.0
31	38.00236582994553	40.0	37.0	41.0	34.0	41.0
32	37.91618055008127	40.0	37.0	41.0	34.0	41.0
33	37.85212389779164	40.0	37.0	41.0	34.0	41.0
34	37.7350176616103	40.0	36.0	41.0	33.0	41.0
35	37.66457111003502	40.0	36.0	41.0	33.0	41.0
36	37.62832351715672	40.0	36.0	41.0	33.0	41.0
37	37.61503601767287	40.0	36.0	41.0	33.0	41.0
38	37.47507385593681	40.0	36.0	41.0	33.0	41.0
39	37.45174307518478	40.0	36.0	41.0	33.0	41.0
40	37.398465448184474	40.0	36.0	41.0	33.0	41.0
41	37.32519322665045	40.0	35.0	41.0	33.0	41.0
42	37.28770969643052	40.0	35.0	41.0	33.0	41.0
43	37.27197847573358	40.0	35.0	41.0	33.0	41.0
44	37.252164063409126	40.0	35.0	41.0	33.0	41.0
45	37.24225967259391	40.0	35.0	41.0	33.0	41.0
46	37.152421949196125	39.0	35.0	41.0	33.0	41.0
47	37.09280791450354	39.0	35.0	41.0	33.0	41.0
48	37.06148999425669	39.0	35.0	41.0	33.0	41.0
49	37.036570419336556	39.0	35.0	41.0	33.0	41.0
50	36.93684332030766	39.0	35.0	41.0	32.0	41.0
51	36.0228249567375	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	11.0
10	14.0
11	15.0
12	20.0
13	28.0
14	24.0
15	26.0
16	57.0
17	94.0
18	165.0
19	298.0
20	527.0
21	872.0
22	1344.0
23	2309.0
24	3375.0
25	4904.0
26	7107.0
27	8122.0
28	8718.0
29	10120.0
30	12519.0
31	16382.0
32	22046.0
33	32885.0
34	57129.0
35	77643.0
36	83142.0
37	128746.0
38	216053.0
39	370556.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58840001951974	20.84126322743875	23.631553658637298	13.938783094404217
2	33.145174307518474	25.808849198752238	24.101153541518862	16.944822952210423
3	31.863159119659755	24.62321272386701	27.243268505275957	16.27035965119727
4	28.979493012308698	26.28098289395151	26.79224991272424	17.94727418101555
5	23.261053990848247	38.15771198624609	21.49048225017549	17.090751772730172
6	27.433867498507862	35.90965739103669	22.831901260149326	13.824573850306122
7	83.5579980255033	3.9077016633070194	8.871158458991655	3.6631418521980352
8	82.90690210475343	4.786746847749787	8.048044835339738	4.258306212157044
9	75.83953648126668	7.3069516548609785	10.384595171867552	6.4689166920047905
10	34.400725233392265	36.39070635179826	16.035278175054522	13.173290239754953
11	29.28486431904263	23.410642762493573	27.078852239327016	20.225640679136777
12	28.646249770079997	20.435290187201808	29.097456052433024	21.821003990285178
13	25.441352567784172	22.2705210644264	31.665052534375388	20.62307383341404
14	20.688577574071783	25.998791277679555	29.096892983029086	24.215738165219577
15	18.927390323464603	26.11675431780388	33.04438488421416	21.911470474517355
16	21.936620907893108	25.864874604443745	30.526901579221988	21.671602908441162
17	21.768169311215967	25.998322053176278	28.618096299883256	23.6154123357245
18	21.078503136296582	25.61656099730853	31.275971576256488	22.028964290138404
19	22.0134798815302	27.280431085935653	27.909942679534677	22.79614635299947
20	22.62619323791184	26.58091119644741	29.274541379970497	21.518354185670262
21	23.623576842081555	27.269263542757617	29.030826172967412	20.076333442193416
22	22.621782527581015	24.557145913805336	28.168860760443998	24.652210798169648
23	20.577652901496638	26.840392346760662	29.598681666835585	22.983273084907115
24	21.881627796108816	24.917979556826843	29.675446795572025	23.524945851492323
25	21.337139682503935	27.108601072834904	27.45676565426788	24.097493590393285
26	20.111619124839994	27.326039707654363	28.702744400274778	23.85959676723086
27	21.58047950990439	26.148286204424227	28.882457385030612	23.388776900640774
28	19.253689043044776	26.5418717177746	29.710075563914007	24.494363675266616
29	20.31244721224338	25.083052737080376	29.23925569732392	25.365244353352328
30	21.738608167509394	26.323870013551204	28.32342331182408	23.61409850711532
31	21.124111758015292	27.1003427215772	26.416964155001743	25.35858136540577
32	21.38443751243445	29.28552123334722	26.04317991568974	23.286861338528585
33	20.809731340818402	27.61630198538272	25.25525812978374	26.318708544015134
34	19.997597570543213	26.898482340266593	27.408341685529493	25.6955784036607
35	20.255107977942693	26.409268873147973	27.477505377312806	25.85811777159653
36	20.682102275926532	27.277709583816634	26.45825591129029	25.581932228966544
37	19.73408108950176	28.382357909435918	26.95009703562728	24.933463965435045
38	20.484183380443476	28.30671891950735	26.18451033607736	25.02458736397182
39	20.277912288802053	28.424400424929708	26.817212656298683	24.480474629969557
40	22.84701028915491	26.30613332732726	28.68266159153444	22.164194791983395
41	20.259706378074828	25.524592994665856	28.42083431870479	25.794866308554525
42	20.87307664876106	25.06578527535971	28.055965344955087	26.005172730924148
43	21.02529307762475	25.022428931256734	28.337406202021793	25.61487178909672
44	20.716355664665894	25.899597217686388	27.36733146394291	26.016715653704807
45	21.026888440935895	24.488920671028577	27.375589815200623	27.108601072834904
46	22.707369076979095	26.113282056479616	26.73143841709929	24.447910449442
47	19.691663194405344	25.419580550832027	30.14026058852014	24.748495666242487
48	20.02697102444847	25.5480542198298	28.359647443477215	26.065327312244506
49	20.866507505715155	24.525426337383678	29.464108079295187	25.143958077605976
50	20.683697639237682	23.58200355109104	30.107039493687992	25.627259315983288
51	19.87756994260446	23.116532843838332	28.48877802677958	28.517119186777627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1325.0
1	1398.5
2	1472.0
3	5674.5
4	9877.0
5	6234.5
6	2592.0
7	2508.5
8	2425.0
9	2407.5
10	2390.0
11	2489.5
12	2589.0
13	2493.0
14	2397.0
15	2356.0
16	2315.0
17	2279.0
18	2243.0
19	2437.0
20	2631.0
21	2785.5
22	2940.0
23	3180.5
24	3421.0
25	3941.0
26	5963.0
27	7465.0
28	9410.5
29	11356.0
30	13563.5
31	15771.0
32	17964.5
33	20158.0
34	23914.5
35	27671.0
36	30094.5
37	32518.0
38	35259.5
39	38001.0
40	41583.5
41	45166.0
42	50911.5
43	56657.0
44	63274.0
45	69891.0
46	85734.5
47	101578.0
48	107848.5
49	114119.0
50	111320.5
51	108522.0
52	93277.5
53	78033.0
54	69562.5
55	61092.0
56	54437.0
57	47782.0
58	45418.0
59	43054.0
60	41585.5
61	40117.0
62	36264.0
63	32411.0
64	28310.5
65	24210.0
66	20040.0
67	15870.0
68	13635.5
69	11401.0
70	9653.5
71	7906.0
72	6307.0
73	4708.0
74	3847.0
75	2363.5
76	1741.0
77	1509.5
78	1278.0
79	933.5
80	589.0
81	387.0
82	185.0
83	190.5
84	196.0
85	116.5
86	37.0
87	34.5
88	32.0
89	18.5
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1065588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.12182298771909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69086853140286	20.586469602196352
2	11.624570141269333	6.770573479247403
3	4.543241813356631	3.9692245163692705
4	2.4785828721734036	2.887234066553049
5	1.593806622980206	2.3207277175541945
6	1.0395043016516197	1.816335616060261
7	0.7654707120130528	1.5604331804269194
8	0.601701037951873	1.4018104895009034
9	0.45262913788284304	1.1863247069257246
>10	5.488002136198652	39.35773915667689
>50	0.6562257711188555	11.669592373923695
>100	0.056698809222347823	2.8568101962973174
>500	0.005476589527134219	1.1053475331740743
>1k	0.0028993709261298803	1.9580468820977852
>5k	3.2215232512554224E-4	0.5533304829961543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5898	0.5534972240678386	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	2930	0.2749655589214593	No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2929	0.2748717140208035	RNA PCR Primer, Index 13 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	2727	0.2559150440883343	RNA PCR Primer, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2507	0.23526916594406094	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	2477	0.2324538189243873	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2349	0.22044167164044637	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	1955	0.18346678078206585	RNA PCR Primer, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1748	0.16404088634631772	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	1249	0.11721228091907943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8768980131157634E-4	0.0	0.0	0.17014080488894395	0.0
2	1.8768980131157634E-4	0.0	0.0	0.8268674196781495	0.0
3	1.8768980131157634E-4	0.0	0.0	1.214353014485899	0.0
4	1.8768980131157634E-4	0.0	0.0	1.7068510531274752	0.0
5	1.8768980131157634E-4	0.0	0.0	3.211466345341727	0.0
6	1.8768980131157634E-4	0.0	0.0	4.274259845268528	0.0
7	1.8768980131157634E-4	0.0	0.0	5.2534375387110215	0.0
8	1.8768980131157634E-4	0.0	0.0	6.821210449066618	0.0
9	1.8768980131157634E-4	0.0	0.0	7.382778334590855	0.0
10	1.8768980131157634E-4	0.0	0.0	8.82939747819983	0.0
11	1.8768980131157634E-4	0.0	0.0	10.49936748536958	0.0
12	1.8768980131157634E-4	0.0	0.0	12.11744126247668	0.0
13	1.8768980131157634E-4	0.0	0.0	12.672627694756322	0.0
14	1.8768980131157634E-4	0.0	0.0	12.898230835932837	0.0
15	2.815347019673645E-4	0.0	0.0	13.338926489412419	0.0
16	2.815347019673645E-4	0.0	0.0	14.104325499161027	0.0
17	2.815347019673645E-4	0.0	0.0	15.009459565986104	0.0
18	2.815347019673645E-4	0.0	0.0	15.944717845921689	0.0
19	2.815347019673645E-4	0.0	0.0	16.58370777448695	0.0
20	2.815347019673645E-4	0.0	0.0	17.215565490602373	0.0
21	2.815347019673645E-4	0.0	0.0	17.99119359452246	0.0
22	2.815347019673645E-4	0.0	0.0	18.816841030492085	0.0
23	2.815347019673645E-4	0.0	0.0	19.619778000503008	0.0
24	2.815347019673645E-4	0.0	0.0	20.235682083506948	0.0
25	2.815347019673645E-4	0.0	0.0	20.800065316050855	0.0
26	2.815347019673645E-4	0.0	0.0	21.32981978025278	0.0
27	2.815347019673645E-4	0.0	0.0	21.824570096510097	0.0
28	2.815347019673645E-4	0.0	0.0	22.347849262566772	0.0
29	2.815347019673645E-4	0.0	0.0	22.847291823856875	0.0
30	2.815347019673645E-4	0.0	0.0	23.456532918914252	0.0
31	2.815347019673645E-4	0.0	0.0	24.013783939008324	0.0
32	2.815347019673645E-4	0.0	0.0	24.51848181473515	0.0
33	2.815347019673645E-4	0.0	0.0	25.03810102966625	0.0
34	3.7537960262315267E-4	0.0	0.0	25.552840309763248	0.0
35	3.7537960262315267E-4	0.0	0.0	26.111968227870435	0.0
36	3.7537960262315267E-4	0.0	0.0	26.63055514889432	0.0
37	3.7537960262315267E-4	0.0	0.0	27.134126885813277	0.0
38	3.7537960262315267E-4	0.0	0.0	27.64417392087749	0.0
39	3.7537960262315267E-4	0.0	0.0	28.177025266801053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	65	0.0	45.000004	4
CGAATAA	35	1.2118107E-7	45.000004	23
CGATATA	35	1.2118107E-7	45.000004	12
TAGACCG	20	7.033055E-4	45.0	1
CGTCTAT	20	7.033055E-4	45.0	13
ATCCGCG	20	7.033055E-4	45.0	1
GCCGATT	50	2.1827873E-11	45.0	9
TCGTCGT	25	3.890614E-5	45.0	42
GTCAACG	20	7.033055E-4	45.0	1
CCGCGTA	20	7.033055E-4	45.0	33
AATAGCG	45	3.8562575E-10	45.0	1
CGGCCTA	60	3.6379788E-12	41.249996	45
CTCGAAC	60	3.6379788E-12	41.249996	13
GTAAGCG	60	3.6379788E-12	41.249996	1
ACTACGG	55	6.184564E-11	40.909092	2
CGTTTTT	2555	0.0	40.861053	1
CGTATGG	90	0.0	40.0	2
GCGTATG	45	1.9281288E-8	40.0	1
CGTTATT	440	0.0	39.886364	1
TAAGCGG	130	0.0	39.807693	2
>>END_MODULE