FastQCFastQC Report
Sat 18 Jun 2016
SRR3554433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407864
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.5073643476926749No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC31400.22303290658756814No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC29930.21259155713904185Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC27640.1963257814675281Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24880.1767216151560094No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC23850.16940556758323247No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG21510.15278464397129268TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT21440.15228743685469617TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG19870.1411357915253178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG850.045.0000042
TCGAACG351.2121927E-745.0000041
TCTACGG800.045.02
TCGAACA207.033829E-445.038
TAATACG950.042.631584
CGTTTTT37950.041.4426881
CGTTATT4900.041.326531
ACGCGAG603.6379788E-1241.2500041
ATTAGCG556.184564E-1140.909091
TACGGGT1000.040.54
ACGGGAT8350.040.14975
TATAGCG451.9288564E-840.01
AGTACGG1800.040.02
CGGTCTA2950.039.6610231
CGAATAT3900.039.2307714
TCACGAC3050.039.09836225
GCTACGA3950.038.73417710
TACGAAC356.249289E-638.5714334
CTCACGT2350.038.2978745
AGCTACG3950.038.1645559