Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554433_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1407864 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7143 | 0.5073643476926749 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC | 3140 | 0.22303290658756814 | No Hit |
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC | 2993 | 0.21259155713904185 | Illumina Single End Adapter 1 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC | 2764 | 0.1963257814675281 | Illumina Single End Adapter 1 (95% over 21bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 2488 | 0.1767216151560094 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC | 2385 | 0.16940556758323247 | No Hit |
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG | 2151 | 0.15278464397129268 | TruSeq Adapter, Index 27 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT | 2144 | 0.15228743685469617 | TruSeq Adapter, Index 27 (95% over 24bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG | 1987 | 0.1411357915253178 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTAACGG | 85 | 0.0 | 45.000004 | 2 |
TCGAACG | 35 | 1.2121927E-7 | 45.000004 | 1 |
TCTACGG | 80 | 0.0 | 45.0 | 2 |
TCGAACA | 20 | 7.033829E-4 | 45.0 | 38 |
TAATACG | 95 | 0.0 | 42.63158 | 4 |
CGTTTTT | 3795 | 0.0 | 41.442688 | 1 |
CGTTATT | 490 | 0.0 | 41.32653 | 1 |
ACGCGAG | 60 | 3.6379788E-12 | 41.250004 | 1 |
ATTAGCG | 55 | 6.184564E-11 | 40.90909 | 1 |
TACGGGT | 100 | 0.0 | 40.5 | 4 |
ACGGGAT | 835 | 0.0 | 40.1497 | 5 |
TATAGCG | 45 | 1.9288564E-8 | 40.0 | 1 |
AGTACGG | 180 | 0.0 | 40.0 | 2 |
CGGTCTA | 295 | 0.0 | 39.66102 | 31 |
CGAATAT | 390 | 0.0 | 39.23077 | 14 |
TCACGAC | 305 | 0.0 | 39.098362 | 25 |
GCTACGA | 395 | 0.0 | 38.734177 | 10 |
TACGAAC | 35 | 6.249289E-6 | 38.57143 | 34 |
CTCACGT | 235 | 0.0 | 38.29787 | 45 |
AGCTACG | 395 | 0.0 | 38.164555 | 9 |