##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1407864
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14588909155998	33.0	31.0	34.0	30.0	34.0
2	32.24647409124745	34.0	31.0	34.0	30.0	34.0
3	32.217528113510966	34.0	31.0	34.0	30.0	34.0
4	35.81838728740844	37.0	35.0	37.0	35.0	37.0
5	31.661154770631253	37.0	35.0	37.0	0.0	37.0
6	33.65580837353608	37.0	35.0	37.0	17.0	37.0
7	35.49940477205185	37.0	35.0	37.0	32.0	37.0
8	35.79472022865845	37.0	35.0	37.0	35.0	37.0
9	37.80255053044896	39.0	38.0	39.0	35.0	39.0
10	37.4049730655802	39.0	37.0	39.0	34.0	39.0
11	37.3374779097981	39.0	37.0	39.0	34.0	39.0
12	37.335349863339076	39.0	37.0	39.0	35.0	39.0
13	37.27418344385537	39.0	37.0	39.0	34.0	39.0
14	38.48548936545007	40.0	38.0	41.0	34.0	41.0
15	38.59660876334646	40.0	38.0	41.0	35.0	41.0
16	38.56979651443606	40.0	38.0	41.0	34.0	41.0
17	38.528929640931224	40.0	38.0	41.0	34.0	41.0
18	38.46972861014984	40.0	38.0	41.0	34.0	41.0
19	38.37887537432593	40.0	37.0	41.0	34.0	41.0
20	38.34333785081513	40.0	37.0	41.0	34.0	41.0
21	38.24080095804708	40.0	37.0	41.0	34.0	41.0
22	38.2901928027139	40.0	37.0	41.0	34.0	41.0
23	38.25854485944665	40.0	37.0	41.0	34.0	41.0
24	38.24227908377514	40.0	37.0	41.0	34.0	41.0
25	38.00655745157203	40.0	36.0	41.0	34.0	41.0
26	38.11104907860418	40.0	37.0	41.0	34.0	41.0
27	38.080368558326654	40.0	37.0	41.0	34.0	41.0
28	38.060031366666095	40.0	37.0	41.0	34.0	41.0
29	38.046584045049805	40.0	37.0	41.0	34.0	41.0
30	37.89999673263895	40.0	36.0	41.0	34.0	41.0
31	37.897326730422826	40.0	36.0	41.0	34.0	41.0
32	37.84024593284579	40.0	36.0	41.0	33.0	41.0
33	37.774088264207336	40.0	37.0	41.0	33.0	41.0
34	37.63288783575686	40.0	36.0	41.0	33.0	41.0
35	37.558112147196034	40.0	36.0	41.0	33.0	41.0
36	37.49190759902945	40.0	36.0	41.0	33.0	41.0
37	37.4942430518857	40.0	36.0	41.0	33.0	41.0
38	37.34171127324798	40.0	36.0	41.0	33.0	41.0
39	37.326371013109224	40.0	36.0	41.0	33.0	41.0
40	37.257889966644505	40.0	35.0	41.0	33.0	41.0
41	37.15674809498645	40.0	35.0	41.0	32.0	41.0
42	37.07949773557673	40.0	35.0	41.0	32.0	41.0
43	37.066539807822345	40.0	35.0	41.0	32.0	41.0
44	37.04811615326481	39.0	35.0	41.0	32.0	41.0
45	37.053367370711946	39.0	35.0	41.0	32.0	41.0
46	36.96742014853707	39.0	35.0	41.0	32.0	41.0
47	36.91676894927351	39.0	35.0	41.0	32.0	41.0
48	36.86875081684026	39.0	35.0	41.0	32.0	41.0
49	36.8303039214015	39.0	35.0	41.0	32.0	41.0
50	36.7333293556764	39.0	35.0	41.0	32.0	41.0
51	35.81652773279237	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	11.0
10	23.0
11	22.0
12	20.0
13	36.0
14	25.0
15	37.0
16	78.0
17	138.0
18	238.0
19	468.0
20	817.0
21	1299.0
22	2058.0
23	3184.0
24	4932.0
25	7621.0
26	10575.0
27	12590.0
28	13538.0
29	14623.0
30	17902.0
31	22916.0
32	30529.0
33	45563.0
34	78279.0
35	101887.0
36	109030.0
37	170861.0
38	281692.0
39	476481.0
40	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.500457430547264	20.15606621094083	24.07995374553224	14.263522612979662
2	33.79736963229403	24.341484688861993	24.63753601200116	17.22360966684282
3	32.13364359057409	24.523036316007797	27.230542154639934	16.112777938778176
4	29.089954711534638	24.999644852059575	27.41102833796446	18.499372098441327
5	22.942912099464152	37.59560582556269	21.704866379138892	17.756615695834256
6	27.47204275412966	35.00281277168818	23.765718847843257	13.759425626338908
7	83.20917361336038	3.905348812101169	9.07985430410892	3.8056232704295296
8	82.76900325599632	4.433951006631323	8.479796343965042	4.317249393407318
9	75.82934147048294	6.870549996306462	11.039844757732281	6.260263775478313
10	35.480557781149315	33.29767648011456	17.105132313916684	14.116633424819444
11	29.599947153986466	23.61151361211026	26.516268616855037	20.27227061704824
12	29.79613087627782	20.113732576442043	28.703766841115335	21.3863697061648
13	25.908610490786042	21.937204161765624	31.332429836972892	20.82175551047544
14	21.485598040719843	24.975778910462942	29.479267883829692	24.05935516498753
15	20.32227544706023	25.536557508395695	32.542845047533	21.598321997011073
16	23.26034332861697	23.668266252990346	31.23348562077019	21.8379047976225
17	22.26102805384611	25.253717688640382	28.420571873419593	24.06468238409392
18	22.98972059801231	24.594989288738116	29.76097123017564	22.654318883073934
19	23.130217123244858	27.46650244625901	27.16079109914026	22.24248933135587
20	24.595770614207055	25.63329980736776	28.435630146093654	21.33529943233153
21	24.41244324735912	26.66493354471739	28.264875016336806	20.657748191586688
22	22.79900615400351	24.415355460470614	28.05398816931181	24.731650216214067
23	21.987990317246553	26.33329639794753	28.58351374848707	23.09519953631885
24	23.07630566588818	23.669189637635455	29.373575856758894	23.880928839717473
25	22.106254581408432	26.02865049465005	26.975119755885512	24.889975168056004
26	21.27364574987357	26.425563832870218	27.769159521090103	24.531630896166106
27	22.049643999704514	25.607374007716654	28.855130893324926	23.487851099253906
28	20.63473460504708	25.654892802145664	29.40802520698022	24.30234738582704
29	21.445963530568292	23.712659745543604	28.316797645227094	26.52457907866101
30	22.002622412392107	24.57403556025298	29.449932663950495	23.973409363404418
31	21.81517532943523	24.90084269503304	29.026809407726883	24.257172567804844
32	22.510839115141803	25.48094134092497	27.567648579692356	24.440570964240866
33	21.803952654517765	24.056300892699863	29.03206559724519	25.107680855537183
34	20.506597228141356	23.35602018376775	30.88700329009052	25.250379298000375
35	21.890182574453217	22.6083627395828	29.026099111846033	26.475355574117955
36	21.166106953512557	24.550950944125287	29.69335106231852	24.58959104004364
37	21.21341265917731	24.81368939045249	30.921807788252277	23.051090162117934
38	21.425720097964007	24.33885659410284	29.16730593295943	25.06811737497372
39	21.92171971156305	23.185336083598983	29.219938857730575	25.67300534710739
40	23.487993158430076	22.544507139894193	31.174460033071377	22.793039668604354
41	20.542325110948216	23.5594489240438	30.505077194956332	25.393148770051653
42	21.499377780808373	23.939954427416286	29.301054647323888	25.259613144451453
43	21.444471909218503	23.43642567748021	29.91496337714438	25.204139036156903
44	21.36783098367456	23.894850638982174	28.85278691691811	25.88453146042515
45	21.33039839075365	22.346263559548365	28.732391765113675	27.590946284584305
46	22.4243961064421	24.00352590875255	28.365310853889298	25.206767130916052
47	20.36922600478455	23.27156600353443	31.575137939460063	24.78407005222095
48	20.654835978475194	22.89816345897047	30.498613502440573	25.94838706011376
49	20.840010114613346	22.34484296778666	30.91371041521056	25.901436502389437
50	20.441818243807642	21.57005222095316	31.87069205548263	26.117437479756568
51	19.96386014558224	21.03548354102385	30.454433098651574	28.546223214742334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	874.5
2	1416.0
3	7189.5
4	12963.0
5	8521.5
6	4080.0
7	3994.0
8	3908.0
9	3849.0
10	3790.0
11	3871.5
12	3953.0
13	3909.5
14	3866.0
15	3718.5
16	3571.0
17	3494.0
18	3417.0
19	3400.0
20	3383.0
21	3560.5
22	3738.0
23	3811.0
24	3884.0
25	4244.0
26	6813.0
27	9022.0
28	10427.5
29	11833.0
30	14344.0
31	16855.0
32	19342.0
33	21829.0
34	26966.5
35	32104.0
36	34234.5
37	36365.0
38	41886.5
39	47408.0
40	52561.5
41	57715.0
42	63151.0
43	68587.0
44	77147.0
45	85707.0
46	105875.0
47	126043.0
48	134831.5
49	143620.0
50	142991.0
51	142362.0
52	127176.5
53	111991.0
54	97460.0
55	82929.0
56	79109.5
57	75290.0
58	70559.0
59	65828.0
60	63027.5
61	60227.0
62	55435.0
63	50643.0
64	43854.0
65	37065.0
66	30820.5
67	24576.0
68	20234.0
69	15892.0
70	13140.0
71	10388.0
72	8671.5
73	6955.0
74	5742.0
75	3442.0
76	2355.0
77	1915.5
78	1476.0
79	1054.5
80	633.0
81	567.5
82	502.0
83	329.5
84	157.0
85	103.0
86	49.0
87	33.5
88	18.0
89	11.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1407864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.053700560433807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72193820341245	20.401194785329047
2	11.313323221523914	6.347611640000686
3	4.231058302448442	3.5609052801187797
4	2.0980834073880006	2.3543601464671053
5	1.2123220167679658	1.7005059420614865
6	0.7817025286232064	1.3157789199197623
7	0.5496900095764633	1.0794587250804064
8	0.4303966383834611	0.9659374732341544
9	0.343193555841203	0.8665064324855664
>10	5.214415328579742	37.36580943180602
>50	1.0334186911066838	17.94693345973872
>100	0.06351904141373073	3.036792243857399
>500	0.004003300923909133	0.8444019451952006
>1k	0.0026688672826060884	1.6789957103211894
>5k	2.668867282606088E-4	0.5348078643845822
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7143	0.5073643476926749	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	3140	0.22303290658756814	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2993	0.21259155713904185	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2764	0.1963257814675281	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2488	0.1767216151560094	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	2385	0.16940556758323247	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	2151	0.15278464397129268	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	2144	0.15228743685469617	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	1987	0.1411357915253178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4205917617042554E-4	0.0	0.0	0.15725950802066108	0.0
2	2.841183523408511E-4	0.0	0.0	0.6886318564861379	0.0
3	2.841183523408511E-4	0.0	0.0	1.0344038912849536	0.0
4	2.841183523408511E-4	0.0	0.0	1.4576692066847365	0.0
5	2.841183523408511E-4	0.0	0.0	2.753461982123273	0.0
6	2.841183523408511E-4	0.0	0.0	3.9346130023922767	0.0
7	3.551479404260639E-4	0.0	0.0	4.888256252024343	0.0
8	3.551479404260639E-4	0.0	0.0	6.344007659830779	0.0
9	3.551479404260639E-4	0.0	0.0	6.92531380872016	0.0
10	3.551479404260639E-4	0.0	0.0	8.226504832853173	0.0
11	3.551479404260639E-4	0.0	0.0	9.753498917509077	0.0
12	3.551479404260639E-4	0.0	0.0	11.096455339436195	0.0
13	4.2617752851127665E-4	0.0	0.0	11.62015649238847	0.0
14	4.2617752851127665E-4	0.0	0.0	11.844538961149656	0.0
15	4.2617752851127665E-4	0.0	0.0	12.227672559281293	0.0
16	4.2617752851127665E-4	0.0	0.0	12.92774017944915	0.0
17	4.2617752851127665E-4	0.0	0.0	13.776969934595956	0.0
18	4.2617752851127665E-4	0.0	0.0	14.592318576226113	0.0
19	4.2617752851127665E-4	0.0	0.0	15.130012558031174	0.0
20	4.2617752851127665E-4	0.0	0.0	15.66479432672474	0.0
21	4.2617752851127665E-4	0.0	0.0	16.341422182824477	0.0
22	4.2617752851127665E-4	0.0	0.0	17.0944778757039	0.0
23	4.2617752851127665E-4	0.0	0.0	17.803069046441987	0.0
24	4.972071165964895E-4	0.0	0.0	18.374217964235182	0.0
25	4.972071165964895E-4	0.0	0.0	18.90274912917725	0.0
26	4.972071165964895E-4	0.0	0.0	19.379357665229026	0.0
27	4.972071165964895E-4	0.0	0.0	19.858807384804216	0.0
28	4.972071165964895E-4	0.0	0.0	20.31275748225681	0.0
29	4.972071165964895E-4	0.0	0.0	20.777504077098357	0.0
30	4.972071165964895E-4	0.0	0.0	21.319104686248103	0.0
31	4.972071165964895E-4	0.0	0.0	21.833998170277813	0.0
32	5.682367046817022E-4	0.0	0.0	22.344061642317723	0.0
33	5.682367046817022E-4	0.0	0.0	22.823866509833337	0.0
34	5.682367046817022E-4	0.0	0.0	23.317095969497053	0.0
35	6.39266292766915E-4	0.0	0.0	23.846479489496144	0.0
36	6.39266292766915E-4	0.0	0.0	24.343047339799867	0.0
37	6.39266292766915E-4	0.0	0.0	24.843095639919763	0.0
38	6.39266292766915E-4	0.0	0.0	25.317147110800477	0.0
39	6.39266292766915E-4	0.0	0.0	25.812436428518662	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	85	0.0	45.000004	2
TCGAACG	35	1.2121927E-7	45.000004	1
TCTACGG	80	0.0	45.0	2
TCGAACA	20	7.033829E-4	45.0	38
TAATACG	95	0.0	42.63158	4
CGTTTTT	3795	0.0	41.442688	1
CGTTATT	490	0.0	41.32653	1
ACGCGAG	60	3.6379788E-12	41.250004	1
ATTAGCG	55	6.184564E-11	40.90909	1
TACGGGT	100	0.0	40.5	4
ACGGGAT	835	0.0	40.1497	5
TATAGCG	45	1.9288564E-8	40.0	1
AGTACGG	180	0.0	40.0	2
CGGTCTA	295	0.0	39.66102	31
CGAATAT	390	0.0	39.23077	14
TCACGAC	305	0.0	39.098362	25
GCTACGA	395	0.0	38.734177	10
TACGAAC	35	6.249289E-6	38.57143	34
CTCACGT	235	0.0	38.29787	45
AGCTACG	395	0.0	38.164555	9
>>END_MODULE