SRR3554431_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1769710
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	22181	1.253369196082974	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21441	1.2115544354724785	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	13637	0.7705782303315233	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	11543	0.6522537590904725	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	6775	0.38283108531906357	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	6052	0.3419769340739443	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	5918	0.3344050720174492	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	5426	0.30660390685479544	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	4888	0.27620344576230005	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	3682	0.20805668725384385	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	3202	0.1809335992902792	TruSeq Adapter, Index 15 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	3031	0.1712709992032593	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	2947	0.16652445880963548	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	2945	0.16641144594312063	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	2850	0.16104333478366512	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	2701	0.15262387622830859	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2444	0.13810172288115002	TruSeq Adapter, Index 14 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	2143	0.12109328647066468	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2065	0.11668578467658544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	2020	0.11414299518000125	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	30	2.166169E-6	45.000004	35
CGTTTTT	12595	0.0	43.39222	1
CGTTATT	1925	0.0	43.01299	1
CGAATAT	315	0.0	41.42857	14
CGGTCTA	200	0.0	40.5	31
CACGACG	195	0.0	40.384617	26
CTACGAA	325	0.0	40.153847	11
GCTACGA	325	0.0	40.153847	10
TCTAGCG	45	1.929402E-8	40.000004	1
CGTTTCT	820	0.0	39.237804	1
TACGAAT	335	0.0	38.955223	12
TAATACG	445	0.0	38.932587	4
GTTATTT	2175	0.0	38.68966	2
GTATACG	105	0.0	38.57143	1
TACGGCT	1950	0.0	38.30769	7
CGTTCTG	600	0.0	37.875	1
TAACGCC	90	0.0	37.500004	12
TTATCGC	60	1.5643309E-10	37.500004	31
AACGCGA	30	1.1401709E-4	37.500004	30
TACGGGA	750	0.0	37.5	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content