##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1769710
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04412813398805	33.0	31.0	34.0	30.0	34.0
2	32.1771211102384	33.0	31.0	34.0	30.0	34.0
3	32.10590040176074	33.0	31.0	34.0	30.0	34.0
4	35.72770227890445	37.0	35.0	37.0	35.0	37.0
5	31.75313751970662	37.0	35.0	37.0	0.0	37.0
6	33.66050878392505	37.0	35.0	37.0	17.0	37.0
7	35.419177153318905	37.0	35.0	37.0	32.0	37.0
8	35.646451113459264	37.0	35.0	37.0	33.0	37.0
9	37.66439981691916	39.0	37.0	39.0	35.0	39.0
10	37.39721649309774	39.0	37.0	39.0	34.0	39.0
11	37.30230207209091	39.0	37.0	39.0	34.0	39.0
12	37.29667685666013	39.0	37.0	39.0	34.0	39.0
13	37.22790287674252	39.0	37.0	39.0	34.0	39.0
14	38.4497267913952	40.0	38.0	41.0	34.0	41.0
15	38.49592419096914	40.0	38.0	41.0	34.0	41.0
16	38.49662374061287	40.0	38.0	41.0	34.0	41.0
17	38.372348011821146	40.0	38.0	41.0	34.0	41.0
18	38.24146894123896	40.0	38.0	41.0	34.0	41.0
19	38.09570155562211	40.0	37.0	41.0	34.0	41.0
20	37.90634849777647	40.0	36.0	41.0	34.0	41.0
21	37.83883404625617	40.0	36.0	41.0	33.0	41.0
22	37.88977064038741	40.0	35.0	41.0	34.0	41.0
23	37.83287996338383	40.0	35.0	41.0	34.0	41.0
24	37.790534607365046	40.0	35.0	41.0	33.0	41.0
25	37.596154737216835	39.0	35.0	41.0	33.0	41.0
26	37.65988608303055	39.0	35.0	41.0	33.0	41.0
27	37.5822038639099	39.0	35.0	41.0	33.0	41.0
28	37.5184623469382	39.0	35.0	41.0	33.0	41.0
29	37.49050013844076	40.0	35.0	41.0	33.0	41.0
30	37.233113900017514	39.0	35.0	41.0	33.0	41.0
31	37.135313130399894	39.0	35.0	41.0	32.0	41.0
32	36.85192150126292	39.0	35.0	41.0	31.0	41.0
33	36.55385967192365	39.0	35.0	41.0	30.0	41.0
34	36.23181933763159	39.0	35.0	41.0	30.0	41.0
35	36.016593114126046	39.0	35.0	41.0	28.0	41.0
36	35.91893304552723	39.0	35.0	41.0	28.0	41.0
37	35.88804493391573	39.0	35.0	41.0	27.0	41.0
38	35.73650089562697	39.0	35.0	41.0	26.0	41.0
39	35.723047278932704	39.0	35.0	41.0	26.0	41.0
40	35.62773335744274	39.0	35.0	41.0	26.0	41.0
41	35.53122828033972	39.0	35.0	41.0	25.0	41.0
42	35.39979940216194	39.0	35.0	41.0	24.0	41.0
43	35.405060151098205	39.0	35.0	41.0	24.0	41.0
44	35.4319148335038	39.0	35.0	41.0	24.0	41.0
45	35.457768786976395	39.0	35.0	41.0	24.0	41.0
46	35.370661859852746	39.0	35.0	41.0	24.0	41.0
47	35.25894807623848	38.0	35.0	41.0	24.0	41.0
48	35.22087686683129	38.0	35.0	40.0	24.0	41.0
49	35.18836645552096	38.0	35.0	40.0	24.0	41.0
50	35.078476134507916	38.0	35.0	40.0	24.0	41.0
51	34.08794943804352	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	26.0
10	26.0
11	24.0
12	22.0
13	17.0
14	52.0
15	73.0
16	122.0
17	261.0
18	521.0
19	1115.0
20	2213.0
21	4096.0
22	6276.0
23	8861.0
24	13560.0
25	21203.0
26	29048.0
27	31634.0
28	30048.0
29	28291.0
30	30454.0
31	35838.0
32	45038.0
33	63154.0
34	100707.0
35	130484.0
36	140769.0
37	207598.0
38	334267.0
39	503523.0
40	381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55947019568178	18.197614298387872	21.471371015590123	19.771544490340226
2	35.75823157466478	26.348836815071397	22.833063044227586	15.059868566036243
3	27.76889998926378	25.923456385509493	31.84843844471693	14.459205180509802
4	24.974995903283588	22.992524198880044	35.549948861677905	16.482531036158466
5	22.97698492973425	34.21515389527098	26.69782054686926	16.110040628125514
6	23.354504410327117	33.28680970328472	29.531561668295936	13.82712421809223
7	72.72084126777834	4.247080030061422	18.36515587299614	4.6669228291641005
8	72.40886924976408	6.922038074034729	15.438066123828198	5.231026552372987
9	66.16180052098932	6.448909708370298	17.26542766894011	10.12386210170028
10	35.34895547858123	26.55288154556396	23.495487961304395	14.602675014550407
11	28.792966079188115	21.218617739629657	31.127642382085202	18.86077379909703
12	25.75568878516819	18.628306332675976	33.565273406377315	22.05073147577852
13	23.40315644936176	19.9425329573772	37.43602059094428	19.218290002316763
14	19.583999638358826	24.68274463047618	34.2743161308915	21.458939600273492
15	18.646162365585322	22.48125399076685	38.960564160229644	19.912019483418185
16	21.866577009792564	23.71038192698239	33.34744110616994	21.07559995705511
17	20.886868469975305	24.64782365472309	33.466161122443786	20.999146752857815
18	21.35225545428347	24.176955546388957	33.15379355939674	21.316995439930835
19	20.78758666674201	26.67482242853349	30.63298506534969	21.904605839374813
20	22.536856321092156	25.453605392973987	32.41209011645976	19.597448169474095
21	22.19646156714942	27.778675602217312	31.82826564804403	18.19659718258924
22	21.148154217357646	22.820010058145122	33.056093936294644	22.975741788202587
23	21.094586118629604	25.268716343355692	32.57189030971176	21.064807228302943
24	22.278960959705262	22.86764498138113	32.07559430641179	22.777799752501824
25	20.448491560764193	27.13670601397969	29.956433539958528	22.458368885297592
26	20.39475394273638	23.810115781681745	32.65077329053913	23.144356985042748
27	22.674505992507246	23.69755496663295	30.924445248091498	22.703493792768306
28	20.514265049075835	24.88825852823344	32.241610207322104	22.355866215368618
29	23.999581852393895	21.896468912985743	31.129620107249213	22.974329127371153
30	22.699255810274	23.867356798571517	32.80074136440434	20.632646026750145
31	25.250408258980283	22.359482627097094	29.48652604098977	22.903583072932854
32	26.094388346113206	23.714902441642984	28.953557362505723	21.237151849738094
33	23.43067508235813	24.804629007012448	28.714648162693322	23.0500477479361
34	25.63668623672805	24.091630832170242	29.231060456232942	21.040622474868766
35	22.3378406631595	24.819207666792863	30.744472258166596	22.09847941188104
36	23.96810776906951	28.772906295381727	27.723638336224575	19.535347599324183
37	23.05908877725729	25.69550943374903	30.648637347361994	20.59676444163168
38	22.183126048900668	27.177390645925037	28.562758870097362	22.076724435076933
39	24.09298698656842	25.83937481282244	27.759519921343045	22.308118279266097
40	22.645235660079898	24.846952325522263	31.637725955099988	20.87008605929785
41	21.474252843686255	26.63871481768199	29.559475846325107	22.32755649230665
42	21.55579162687672	24.774567584519495	31.6880166806991	21.981624107904686
43	22.33586293799549	25.055969622141482	29.447423589175624	23.1607438506874
44	22.824078521339654	23.883404625616627	29.19246656231812	24.1000502907256
45	21.819337631589356	22.45379186420374	29.41877482751411	26.308095676692787
46	24.057105401449956	24.601827418051545	28.344926569889985	22.996140610608517
47	20.938684869272368	23.37286900113578	32.884879443524646	22.80356668606721
48	21.649196761051247	23.11361748535071	31.016042176401783	24.221143577196262
49	21.742827920958803	21.760118889535573	32.603477405902666	23.893575783602962
50	20.711698526877285	22.057794779935698	32.76954981324624	24.46095687994078
51	20.455667877787885	21.49962423221884	30.933825316012225	27.110882573981048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	553.0
1	1204.0
2	1855.0
3	21009.0
4	40163.0
5	27005.5
6	13848.0
7	13664.5
8	13481.0
9	13375.0
10	13269.0
11	13200.0
12	13131.0
13	12706.0
14	12281.0
15	11524.0
16	10767.0
17	10224.0
18	9681.0
19	9191.0
20	8701.0
21	8457.0
22	8213.0
23	8303.0
24	8393.0
25	8880.5
26	10822.0
27	12276.0
28	14214.0
29	16152.0
30	18749.5
31	21347.0
32	24190.5
33	27034.0
34	31872.5
35	36711.0
36	39224.0
37	41737.0
38	46304.0
39	50871.0
40	57177.0
41	63483.0
42	69817.0
43	76151.0
44	90382.0
45	104613.0
46	156057.5
47	207502.0
48	189127.0
49	170752.0
50	167571.0
51	164390.0
52	143957.5
53	123525.0
54	110937.0
55	98349.0
56	90325.5
57	82302.0
58	79359.0
59	76416.0
60	71655.0
61	66894.0
62	60575.5
63	54257.0
64	45688.5
65	37120.0
66	31036.5
67	24953.0
68	20805.0
69	16657.0
70	14360.5
71	12064.0
72	10490.0
73	8916.0
74	6905.0
75	3896.0
76	2898.0
77	2413.5
78	1929.0
79	1476.5
80	1024.0
81	770.0
82	516.0
83	327.0
84	138.0
85	123.0
86	108.0
87	64.5
88	21.0
89	11.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1769710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.23623830378659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4299564729647	23.561480956337757
2	9.37093741356507	5.854256683599762
3	3.503574477514451	3.2831546188411798
4	1.8810160785440788	2.35023465930628
5	1.2145627889842214	1.8969186355811407
6	0.8847242895236763	1.6581275244425902
7	0.6585064713181973	1.4398485543876576
8	0.5615467911084429	1.4032487508631997
9	0.44866343179750984	1.2613102086439545
>10	5.601946260461044	39.07017371218885
>50	0.3961173963634384	7.612228261559083
>100	0.04255836261595277	2.3869770470875227
>500	0.0011399545624343644	0.23014567766982716
>1k	0.003229871260230699	2.4742737476561203
>5k	7.599697082895763E-4	1.4344639440323343
>10k+	7.599697082895763E-4	4.083157017802849
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	22181	1.253369196082974	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21441	1.2115544354724785	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	13637	0.7705782303315233	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	11543	0.6522537590904725	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	6775	0.38283108531906357	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	6052	0.3419769340739443	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	5918	0.3344050720174492	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	5426	0.30660390685479544	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	4888	0.27620344576230005	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	3682	0.20805668725384385	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	3202	0.1809335992902792	TruSeq Adapter, Index 15 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	3031	0.1712709992032593	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	2947	0.16652445880963548	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	2945	0.16641144594312063	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	2850	0.16104333478366512	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	2701	0.15262387622830859	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2444	0.13810172288115002	TruSeq Adapter, Index 14 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	2143	0.12109328647066468	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2065	0.11668578467658544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	2020	0.11414299518000125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1301286651485272E-4	0.0	0.0	0.14397839193992237	0.0
2	1.1301286651485272E-4	0.0	0.0	0.7383695633747902	0.0
3	1.1301286651485272E-4	0.0	0.0	1.2351176181408254	0.0
4	1.1301286651485272E-4	0.0	0.0	2.0319148335037944	0.0
5	1.1301286651485272E-4	0.0	0.0	4.274881195224077	0.0
6	1.1301286651485272E-4	0.0	0.0	5.888817941922688	0.0
7	1.1301286651485272E-4	0.0	0.0	7.210898960846692	0.0
8	1.1301286651485272E-4	0.0	0.0	9.112227427092574	0.0
9	1.6951929977227908E-4	0.0	0.0	10.08475964988614	0.0
10	1.6951929977227908E-4	0.0	0.0	12.148092060281064	0.0
11	1.6951929977227908E-4	0.0	0.0	13.384565832820067	0.0
12	1.6951929977227908E-4	0.0	0.0	15.306914692237712	0.0
13	1.6951929977227908E-4	0.0	0.0	15.942273027784212	0.0
14	1.6951929977227908E-4	0.0	0.0	16.275717490436286	0.0
15	1.6951929977227908E-4	0.0	0.0	16.70883930135446	0.0
16	1.6951929977227908E-4	0.0	0.0	17.232597431217545	0.0
17	1.6951929977227908E-4	0.0	0.0	17.795740545061054	0.0
18	1.6951929977227908E-4	0.0	0.0	18.455792191941054	0.0
19	1.6951929977227908E-4	0.0	0.0	19.07877561860418	0.0
20	1.6951929977227908E-4	0.0	0.0	19.56179261008866	0.0
21	1.6951929977227908E-4	0.0	0.0	20.080860705991377	0.0
22	1.6951929977227908E-4	0.0	0.0	20.659599595413937	0.0
23	1.6951929977227908E-4	0.0	0.0	21.200422668120765	0.0
24	1.6951929977227908E-4	0.0	0.0	21.63834752586582	0.0
25	2.2602573302970544E-4	0.0	0.0	22.052257149476468	0.0
26	2.2602573302970544E-4	0.0	0.0	22.449666894575948	0.0
27	2.825321662871318E-4	0.0	0.0	22.867588474947873	0.0
28	2.825321662871318E-4	0.0	0.0	23.254205491295185	0.0
29	2.825321662871318E-4	0.0	0.0	23.678060247159138	0.0
30	2.825321662871318E-4	0.0	0.0	24.195659175797164	0.0
31	2.825321662871318E-4	0.0	0.0	24.633640539975477	0.0
32	2.825321662871318E-4	0.0	0.0	25.05664769934057	0.0
33	2.825321662871318E-4	0.0	0.0	25.475077837611813	0.0
34	2.825321662871318E-4	0.0	0.0	25.868927677416075	0.0
35	2.825321662871318E-4	0.0	0.0	26.307756638093245	0.0
36	3.3903859954455815E-4	0.0	0.0	26.72680834713032	0.0
37	3.3903859954455815E-4	0.0	0.0	27.147555249165116	0.0
38	3.3903859954455815E-4	0.0	0.0	27.62367845579219	0.0
39	3.955450328019845E-4	0.0	0.0	28.115679970164603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	30	2.166169E-6	45.000004	35
CGTTTTT	12595	0.0	43.39222	1
CGTTATT	1925	0.0	43.01299	1
CGAATAT	315	0.0	41.42857	14
CGGTCTA	200	0.0	40.5	31
CACGACG	195	0.0	40.384617	26
CTACGAA	325	0.0	40.153847	11
GCTACGA	325	0.0	40.153847	10
TCTAGCG	45	1.929402E-8	40.000004	1
CGTTTCT	820	0.0	39.237804	1
TACGAAT	335	0.0	38.955223	12
TAATACG	445	0.0	38.932587	4
GTTATTT	2175	0.0	38.68966	2
GTATACG	105	0.0	38.57143	1
TACGGCT	1950	0.0	38.30769	7
CGTTCTG	600	0.0	37.875	1
TAACGCC	90	0.0	37.500004	12
TTATCGC	60	1.5643309E-10	37.500004	31
AACGCGA	30	1.1401709E-4	37.500004	30
TACGGGA	750	0.0	37.5	4
>>END_MODULE