SRR3554427_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750133
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2860	0.3812657222119277	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2546	0.3394064785844644	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC	1956	0.2607537596666191	No Hit
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	1828	0.24369011895223913	TruSeq Adapter, Index 14 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTC	1695	0.22595992977245372	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG	1647	0.21956106450456117	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.12491118241698472	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	843	0.11238007126736192	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	130	0.0	45.000004	31
ACCGGCA	25	3.8895058E-5	45.0	22
GTTAGCG	40	6.8102963E-9	45.0	1
TAACGCC	45	3.8380676E-10	45.0	12
GTCCGTT	20	7.031719E-4	45.0	32
TATAGCG	35	1.2111741E-7	45.0	1
TATCGCG	25	3.8895058E-5	45.0	1
TACGTAG	40	6.8102963E-9	45.0	1
ACGCTAG	30	2.16449E-6	44.999996	1
CGCGTCA	30	2.16449E-6	44.999996	39
TCACGAC	145	0.0	41.89655	25
CTAACGG	50	1.0804797E-9	40.5	2
CACGACG	140	0.0	40.17857	26
ATAACGG	45	1.9268555E-8	40.0	2
TCGCGAG	45	1.9268555E-8	40.0	1
AATTGCG	40	3.45608E-7	39.375	1
CAAGGCG	40	3.45608E-7	39.375	1
TGGTACG	40	3.45608E-7	39.375	1
CTCACGA	155	0.0	39.19355	24
ACGCGAG	35	6.245389E-6	38.571426	1