##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750133
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11929084575669	33.0	31.0	34.0	30.0	34.0
2	32.22443220068975	34.0	31.0	34.0	30.0	34.0
3	32.23357724563511	34.0	31.0	34.0	30.0	34.0
4	35.81040028901541	37.0	35.0	37.0	35.0	37.0
5	35.901413482675736	37.0	35.0	37.0	35.0	37.0
6	35.900734936337955	37.0	35.0	37.0	35.0	37.0
7	36.12696548478736	37.0	35.0	37.0	35.0	37.0
8	35.93660590855222	37.0	35.0	37.0	35.0	37.0
9	37.74934978197199	39.0	38.0	39.0	35.0	39.0
10	37.56451189322427	39.0	37.0	39.0	35.0	39.0
11	37.56240026768587	39.0	37.0	39.0	35.0	39.0
12	37.465889382282874	39.0	37.0	39.0	35.0	39.0
13	37.44179232216154	39.0	37.0	39.0	35.0	39.0
14	38.65282823179356	40.0	38.0	41.0	35.0	41.0
15	38.76778384633125	40.0	38.0	41.0	35.0	41.0
16	38.81952933679761	40.0	38.0	41.0	35.0	41.0
17	38.76454442078938	40.0	38.0	41.0	35.0	41.0
18	38.75142408079634	40.0	38.0	41.0	35.0	41.0
19	38.74383475996923	40.0	38.0	41.0	35.0	41.0
20	38.705768177109924	40.0	38.0	41.0	35.0	41.0
21	38.65956170439109	40.0	38.0	41.0	35.0	41.0
22	38.67697861579213	40.0	38.0	41.0	35.0	41.0
23	38.6389626906162	40.0	38.0	41.0	35.0	41.0
24	38.596188942494194	40.0	38.0	41.0	35.0	41.0
25	38.50748600581497	40.0	38.0	41.0	34.0	41.0
26	38.49615868119387	40.0	38.0	41.0	34.0	41.0
27	38.459521178244394	40.0	38.0	41.0	34.0	41.0
28	38.42158657198124	40.0	38.0	41.0	34.0	41.0
29	38.42981178004434	40.0	38.0	41.0	34.0	41.0
30	38.29732860706035	40.0	38.0	41.0	34.0	41.0
31	38.317070439508726	40.0	38.0	41.0	34.0	41.0
32	38.226141230955044	40.0	38.0	41.0	34.0	41.0
33	38.18750808190014	40.0	38.0	41.0	34.0	41.0
34	38.118940241263886	40.0	37.0	41.0	34.0	41.0
35	38.057581788829445	40.0	37.0	41.0	34.0	41.0
36	38.03041727267031	40.0	37.0	41.0	34.0	41.0
37	38.00443654658574	40.0	37.0	41.0	34.0	41.0
38	37.91532034985796	40.0	37.0	41.0	33.0	41.0
39	37.85397256219897	40.0	37.0	41.0	33.0	41.0
40	37.77530117992409	40.0	37.0	41.0	33.0	41.0
41	37.68518516049821	40.0	37.0	41.0	33.0	41.0
42	37.68485721865323	40.0	36.0	41.0	33.0	41.0
43	37.63943327383277	40.0	36.0	41.0	33.0	41.0
44	37.62383737283922	40.0	36.0	41.0	33.0	41.0
45	37.54181991726801	40.0	36.0	41.0	33.0	41.0
46	37.41338002727516	40.0	36.0	41.0	33.0	41.0
47	37.37096754842141	40.0	35.0	41.0	33.0	41.0
48	37.32575156672217	39.0	35.0	41.0	33.0	41.0
49	37.25483480929382	39.0	35.0	41.0	33.0	41.0
50	37.172653382800114	39.0	35.0	41.0	32.0	41.0
51	36.38626750189633	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	17.0
10	20.0
11	18.0
12	18.0
13	17.0
14	33.0
15	43.0
16	72.0
17	93.0
18	143.0
19	214.0
20	415.0
21	591.0
22	944.0
23	1459.0
24	2045.0
25	2831.0
26	3788.0
27	4530.0
28	5239.0
29	6266.0
30	7752.0
31	10087.0
32	13590.0
33	18830.0
34	34442.0
35	47644.0
36	47073.0
37	73553.0
38	141195.0
39	326671.0
40	488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91185696403171	22.055555481494615	23.243478156540238	11.789109397933434
2	31.61559350141908	26.101637976198887	24.87665520647672	17.406113315905316
3	31.716908868160715	25.188066649514152	26.606881712976232	16.4881427693489
4	29.449710917930556	25.657716698238847	27.07479873569087	17.817773648139728
5	26.559823391318606	30.511389313628385	24.28356038195893	18.645226913094078
6	27.37221266095479	35.037120084038435	22.83515056663285	14.755516688373929
7	81.58886490795632	5.399709118249698	8.430371680755279	4.581054293038702
8	81.29438379594018	5.787906944501842	7.584121749076497	5.3335875104814745
9	74.51265308951879	8.703923170957683	9.803594829183625	6.9798289103399
10	33.34941936963178	36.48832940291922	16.219257118404336	13.942994109044662
11	30.46979668938708	25.286182583621837	24.619367498830208	19.624653228160874
12	28.818489521191577	22.01556257357029	27.620835238551027	21.545112666687107
13	26.09683882724797	22.737701180990573	30.548316098611846	20.617143893149613
14	21.781604062212967	27.19451084007769	27.503789328025828	23.52009576968351
15	19.844347602358514	26.367057575123344	33.06573634275522	20.722858479762923
16	23.096170945685632	25.401495468136986	29.94535635680606	21.556977229371324
17	22.557599785638015	25.206863316238586	28.589063539399014	23.64647335872439
18	22.946997399127888	25.11834568003274	30.029741392526393	21.90491552831298
19	23.64314061639736	27.044804054747623	27.279562424263432	22.032492904591585
20	25.51214251339429	26.46024105058703	28.025696776438313	20.001919659580366
21	24.45259707278576	26.826442777480793	28.03289549986469	20.688064649868757
22	22.809021866788957	25.098082606684414	27.53685013190994	24.55604539461669
23	21.677755811302795	27.490991597490044	28.00476715462458	22.82648543658258
24	22.65118319018094	24.720816175264922	28.209264223810976	24.41873641074316
25	21.911847632353197	27.53631689313762	26.503833320224544	24.04800215428464
26	21.055332854307167	28.31991126906829	27.486192448539125	23.13856342808542
27	22.462683284164275	28.227261032376926	27.409006136245175	21.901049547213628
28	19.117943084759638	29.09230763078014	28.984060159998293	22.805689124461928
29	21.75280916850745	26.830442068273225	28.01249911682328	23.40424964639604
30	21.86638902701254	27.92051542859733	27.079731194334872	23.133364350055256
31	22.292980044872042	29.39092134328179	24.948109201968187	23.367989409877982
32	22.157937325780892	29.67740387371306	25.617590480621438	22.54706831988461
33	21.411936283299095	28.05195878597529	24.722549201274973	25.813555729450645
34	21.543113021290893	27.704153796726715	27.00614424375411	23.74658893822829
35	20.097102780440267	26.608481429293207	27.06666684441292	26.2277489458536
36	20.000586562649556	29.874168980700755	25.40709447524639	24.718149981403297
37	21.09452590407301	29.06751202786706	27.9411784310249	21.896783637035032
38	19.44175232925361	27.394475379699333	26.821776938222957	26.3419953528241
39	22.56973096770839	23.855902886554784	27.531917673265944	26.04244847247088
40	22.69410891135305	23.920558087699114	28.49508020577684	24.89025279517099
41	20.516761694259554	26.04058213676775	26.402517953482917	27.040138215489783
42	20.691130772809622	26.866702304791286	26.845239444205227	25.596927478193866
43	21.243832760323837	26.796181477151386	27.193311052839963	24.766674709684818
44	21.50125377766343	25.75823220682199	27.395941786323224	25.34457222919136
45	21.417135361329258	24.495256174571708	27.22010630114926	26.86750216294977
46	22.445752953142975	26.369190530212645	26.196687787365708	24.988368729278672
47	20.03644687008837	26.302802303058257	29.32466642582049	24.336084401032885
48	20.02044970691864	25.688511237340578	27.80573578285451	26.485303272886274
49	20.768450394796655	23.859635557961052	29.924026805913083	25.447887241329205
50	20.39345022815954	23.335728464152357	29.147631153408792	27.123190154279307
51	20.090303986093133	22.871277493457825	27.090929208553682	29.94748931189536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	340.0
1	728.5
2	1117.0
3	1618.5
4	2120.0
5	1614.5
6	1109.0
7	1068.5
8	1028.0
9	976.5
10	925.0
11	924.5
12	924.0
13	994.0
14	1064.0
15	1066.0
16	1068.0
17	1114.0
18	1160.0
19	1299.0
20	1438.0
21	1725.5
22	2013.0
23	2358.5
24	2704.0
25	3125.0
26	4822.0
27	6098.0
28	6976.5
29	7855.0
30	9240.0
31	10625.0
32	12471.5
33	14318.0
34	16099.0
35	17880.0
36	19667.0
37	21454.0
38	23624.0
39	25794.0
40	29792.5
41	33791.0
42	37544.0
43	41297.0
44	45661.5
45	50026.0
46	61928.5
47	73831.0
48	79181.5
49	84532.0
50	83482.0
51	82432.0
52	70784.5
53	59137.0
54	51209.0
55	43281.0
56	38811.5
57	34342.0
58	33178.0
59	32014.0
60	30651.5
61	29289.0
62	26267.5
63	23246.0
64	19227.5
65	15209.0
66	12049.5
67	8890.0
68	7070.5
69	5251.0
70	4375.0
71	3499.0
72	2874.5
73	2250.0
74	1920.5
75	1258.0
76	925.0
77	656.0
78	387.0
79	285.0
80	183.0
81	129.5
82	76.0
83	54.5
84	33.0
85	27.5
86	22.0
87	16.5
88	11.0
89	6.0
90	1.0
91	2.0
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	750133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.08373413497976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.41024637090479	20.881193980657432
2	12.071083533176335	7.26286535466418
3	4.430072028506285	3.9981932731318066
4	2.2922890478884406	2.7584245710880695
5	1.3853155396657038	2.083773219417952
6	0.9536163030342021	1.7213003596357936
7	0.7427742352049195	1.5641795829952405
8	0.5828095735921132	1.4026470610612842
9	0.5424000039790892	1.4685675763066994
>10	7.38786854246193	48.687195524901895
>50	0.13732222359432134	2.6662111407083797
>100	0.05796067874271062	3.043515290968447
>500	0.0035668109995514227	0.781031666268973
>1k	0.002675108249663567	1.680901398193847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2860	0.3812657222119277	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2546	0.3394064785844644	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC	1956	0.2607537596666191	No Hit
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	1828	0.24369011895223913	TruSeq Adapter, Index 14 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTC	1695	0.22595992977245372	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG	1647	0.21956106450456117	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.12491118241698472	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	843	0.11238007126736192	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6661938616218725E-4	0.0	0.0	0.249155816368564	0.0
2	2.6661938616218725E-4	0.0	0.0	1.2035199091361133	0.0
3	2.6661938616218725E-4	0.0	0.0	1.6494408324923713	0.0
4	3.9992907924328087E-4	0.0	0.0	2.1593504085275543	0.0
5	3.9992907924328087E-4	0.0	0.0	3.6928118080393744	0.0
6	3.9992907924328087E-4	0.0	0.0	4.474539848266907	0.0
7	3.9992907924328087E-4	0.0	0.0	5.360915997563099	0.0
8	3.9992907924328087E-4	0.0	0.0	6.907708366383028	0.0
9	3.9992907924328087E-4	0.0	0.0	7.454411417708593	0.0
10	3.9992907924328087E-4	0.0	0.0	8.947080051137599	0.0
11	3.9992907924328087E-4	0.0	0.0	10.66130939446738	0.0
12	3.9992907924328087E-4	0.0	0.0	12.36634036897457	0.0
13	3.9992907924328087E-4	0.0	0.0	12.943971269094947	0.0
14	3.9992907924328087E-4	0.0	0.0	13.173663870273671	0.0
15	3.9992907924328087E-4	0.0	0.0	13.627316755828634	0.0
16	3.9992907924328087E-4	0.0	0.0	14.46183543451628	0.0
17	3.9992907924328087E-4	0.0	0.0	15.499118156380268	0.0
18	3.9992907924328087E-4	0.0	0.0	16.615453526241346	0.0
19	3.9992907924328087E-4	0.0	0.0	17.345324095860335	0.0
20	3.9992907924328087E-4	0.0	0.0	18.07879402719251	0.0
21	5.332387723243745E-4	0.0	0.0	18.94903970362589	0.0
22	5.332387723243745E-4	0.0	0.0	19.911002448899062	0.0
23	5.332387723243745E-4	0.0	0.0	20.78831353906574	0.0
24	5.332387723243745E-4	0.0	0.0	21.477391342601912	0.0
25	5.332387723243745E-4	0.0	0.0	22.12460990251062	0.0
26	5.332387723243745E-4	0.0	0.0	22.686910187926674	0.0
27	5.332387723243745E-4	0.0	0.0	23.28613725832619	0.0
28	5.332387723243745E-4	0.0	0.0	23.872166669110676	0.0
29	5.332387723243745E-4	0.0	0.0	24.474993101223383	0.0
30	5.332387723243745E-4	0.0	0.0	25.11208012445793	0.0
31	5.332387723243745E-4	0.0	0.0	25.75556601296037	0.0
32	5.332387723243745E-4	0.0	0.0	26.34092887527945	0.0
33	5.332387723243745E-4	0.0	0.0	26.916826749389774	0.0
34	5.332387723243745E-4	0.0	0.0	27.4708618338348	0.0
35	5.332387723243745E-4	0.0	0.0	28.080087131215397	0.0
36	5.332387723243745E-4	0.0	0.0	28.682913563328103	0.0
37	5.332387723243745E-4	0.0	0.0	29.274275361835834	0.0
38	5.332387723243745E-4	0.0	0.0	29.843641060985185	0.0
39	5.332387723243745E-4	0.0	0.0	30.406474585173562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	130	0.0	45.000004	31
ACCGGCA	25	3.8895058E-5	45.0	22
GTTAGCG	40	6.8102963E-9	45.0	1
TAACGCC	45	3.8380676E-10	45.0	12
GTCCGTT	20	7.031719E-4	45.0	32
TATAGCG	35	1.2111741E-7	45.0	1
TATCGCG	25	3.8895058E-5	45.0	1
TACGTAG	40	6.8102963E-9	45.0	1
ACGCTAG	30	2.16449E-6	44.999996	1
CGCGTCA	30	2.16449E-6	44.999996	39
TCACGAC	145	0.0	41.89655	25
CTAACGG	50	1.0804797E-9	40.5	2
CACGACG	140	0.0	40.17857	26
ATAACGG	45	1.9268555E-8	40.0	2
TCGCGAG	45	1.9268555E-8	40.0	1
AATTGCG	40	3.45608E-7	39.375	1
CAAGGCG	40	3.45608E-7	39.375	1
TGGTACG	40	3.45608E-7	39.375	1
CTCACGA	155	0.0	39.19355	24
ACGCGAG	35	6.245389E-6	38.571426	1
>>END_MODULE