FastQCFastQC Report
Sat 18 Jun 2016
SRR3554426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018395
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.33022550189268407No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC18990.18646988643895543No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC15100.14827252686825837No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT11530.1132173665424516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG207.032908E-445.01
TAACGCG351.211738E-745.01
TGCGATA207.032908E-445.01
TATCGCG351.211738E-745.01
TATAGCG750.042.01
TCACGAC2000.041.62525
CGGTCTA2000.041.62531
ACGGTCT2050.040.60975630
ATAGACG451.9279469E-840.0000041
CACGACG2100.039.64285726
GCGCGAC1050.038.571439
ACGGGTA1050.038.571435
CGTTTTT18000.037.751
ACCGCTC301.1398307E-437.49999618
ATTCGCT301.1398307E-437.49999610
TCGTAAG301.1398307E-437.4999961
TATGCGG1450.037.2413832
CGACGGT2250.037.028
TTTACGG1050.036.428572
AACGTAA250.002106995836.041
Produced by FastQC (version 0.11.5)