##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018395
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.209033822829056	33.0	31.0	34.0	30.0	34.0
2	32.31276862121279	34.0	31.0	34.0	30.0	34.0
3	32.31495539549978	34.0	31.0	34.0	30.0	34.0
4	35.880412806425795	37.0	35.0	37.0	35.0	37.0
5	35.95327451529122	37.0	35.0	37.0	35.0	37.0
6	35.95184579657206	37.0	35.0	37.0	35.0	37.0
7	36.148393305151735	37.0	36.0	37.0	35.0	37.0
8	35.98861934710991	37.0	36.0	37.0	35.0	37.0
9	37.79514039248033	39.0	38.0	39.0	35.0	39.0
10	37.620584350865826	39.0	37.0	39.0	35.0	39.0
11	37.62724974101405	39.0	37.0	39.0	35.0	39.0
12	37.49082232336176	39.0	37.0	39.0	35.0	39.0
13	37.444571114351504	39.0	37.0	39.0	35.0	39.0
14	38.629497395411406	40.0	38.0	41.0	35.0	41.0
15	38.77607804437375	40.0	38.0	41.0	35.0	41.0
16	38.827787842634734	40.0	38.0	41.0	35.0	41.0
17	38.78823737351421	40.0	38.0	41.0	35.0	41.0
18	38.75571462939233	40.0	38.0	41.0	35.0	41.0
19	38.7328237078933	40.0	38.0	41.0	35.0	41.0
20	38.70511442023969	40.0	38.0	41.0	35.0	41.0
21	38.637910633889604	40.0	38.0	41.0	35.0	41.0
22	38.65256015593164	40.0	38.0	41.0	35.0	41.0
23	38.6347114822834	40.0	38.0	41.0	35.0	41.0
24	38.59814708438278	40.0	38.0	41.0	35.0	41.0
25	38.49807294811934	40.0	38.0	41.0	34.0	41.0
26	38.49235512743091	40.0	38.0	41.0	35.0	41.0
27	38.454732201159665	40.0	38.0	41.0	35.0	41.0
28	38.408327809936225	40.0	38.0	41.0	34.0	41.0
29	38.3938962779668	40.0	38.0	41.0	34.0	41.0
30	38.23980871862097	40.0	37.0	41.0	34.0	41.0
31	38.22270729923065	40.0	37.0	41.0	34.0	41.0
32	38.13048964301671	40.0	37.0	41.0	34.0	41.0
33	38.058734577447844	40.0	37.0	41.0	34.0	41.0
34	37.956706386029	40.0	37.0	41.0	34.0	41.0
35	37.89560730364937	40.0	37.0	41.0	33.0	41.0
36	37.85346353821454	40.0	37.0	41.0	33.0	41.0
37	37.839884327790294	40.0	37.0	41.0	33.0	41.0
38	37.73942919986842	40.0	37.0	41.0	33.0	41.0
39	37.67375428983842	40.0	36.0	41.0	33.0	41.0
40	37.606430707142124	40.0	36.0	41.0	33.0	41.0
41	37.513598358200895	40.0	36.0	41.0	33.0	41.0
42	37.50747892517147	40.0	36.0	41.0	33.0	41.0
43	37.46793434767453	40.0	36.0	41.0	33.0	41.0
44	37.44476553792978	40.0	36.0	41.0	33.0	41.0
45	37.38121063045282	40.0	35.0	41.0	33.0	41.0
46	37.28299431949293	40.0	35.0	41.0	33.0	41.0
47	37.253858276994684	40.0	35.0	41.0	33.0	41.0
48	37.187825941800575	39.0	35.0	41.0	32.0	41.0
49	37.12316733683885	39.0	35.0	41.0	32.0	41.0
50	37.053137535042886	39.0	35.0	41.0	32.0	41.0
51	36.25674713642545	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	26.0
10	19.0
11	30.0
12	27.0
13	43.0
14	39.0
15	59.0
16	74.0
17	115.0
18	194.0
19	347.0
20	516.0
21	889.0
22	1283.0
23	1984.0
24	3145.0
25	4543.0
26	6201.0
27	7252.0
28	7934.0
29	9119.0
30	10887.0
31	14044.0
32	18389.0
33	25525.0
34	48184.0
35	64011.0
36	64061.0
37	96528.0
38	184618.0
39	447629.0
40	668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.325615306438074	21.380603793223653	25.30579981244998	11.987981087888294
2	32.51695069202029	24.558741941977328	25.971258696281897	16.95304866972049
3	32.18584144658998	24.211037956784942	26.96772863181771	16.63539196480737
4	29.080563042827194	25.801972711963433	26.549226969888895	18.568237275320477
5	26.138089837440287	29.92905503267396	24.95220420367343	18.980650926212324
6	27.382891707048834	34.26911954595221	23.64524570525189	14.702743041747063
7	82.09182095355928	5.428541970453508	7.956735844146917	4.522901231840297
8	83.280357817939	4.952106009947024	7.381222413699989	4.386313758413975
9	75.70235517652777	8.96341792722863	9.488950750936523	5.845276145307076
10	33.00968681110964	38.83777905429622	15.263821994412776	12.888712140181363
11	30.902253055052313	24.780168795015687	25.778504411353158	18.539073738578843
12	28.908527634169452	22.470946931200565	28.040593286494925	20.579932148135054
13	26.507789217346904	22.352525297158763	30.861208077415935	20.2784774080784
14	22.02053230819083	25.983434718355845	30.157060865381315	21.838972108072017
15	20.976242027896838	25.4422890921499	32.81428129556802	20.767187584385233
16	24.91410503782913	25.587615807226076	29.548652536589437	19.949626618355353
17	24.546173144997766	25.099789374456865	28.703008164808352	21.651029315737016
18	24.438356433407467	24.799905734022655	30.110909813971986	20.650828018597892
19	23.816593757824815	26.596065377383038	28.55944893680743	21.027891927984722
20	25.1741220253438	26.582711030592254	28.058268157247433	20.18489878681651
21	24.776928402044394	26.115407086641234	29.578601623142298	19.529062888172074
22	23.408304243441886	24.0146505039793	29.816426828489927	22.760618424088886
23	21.974774031687115	26.421182350659617	29.567014763426762	22.037028854226502
24	22.436284545780367	25.381507175506556	28.93170135360052	23.250506925112553
25	21.93765680310685	26.849306997775912	27.910781180190398	23.30225501892684
26	21.054698815292692	27.403217808414222	28.405579367534205	23.13650400875888
27	21.5390884676378	26.53695275408854	29.487968813672495	22.43598996460116
28	19.825313360729382	27.082713485435416	29.632215397758237	23.459757756076964
29	21.979585524280854	26.302858910344217	28.392028633290618	23.32552693208431
30	22.716725828386824	25.8356531601196	29.119644145935514	22.32797686555806
31	22.758261774655217	27.00278379214352	27.617574713151576	22.621379720049685
32	22.91193495647563	27.736880090731002	26.887504357346607	22.463680595446757
33	22.15741436279636	26.81444822490291	27.418339642280255	23.609797770020474
34	21.43598505491484	27.610210183671363	28.18631277647671	22.76749198493708
35	20.7625724792443	26.81091325075241	27.784896822941985	24.641617447061307
36	21.650636540831407	28.724708978343372	27.132203123542435	22.492451357282782
37	21.863324152219914	27.96164553046706	27.627295892065458	22.54773442524757
38	20.341517780429008	27.98913977385985	26.39653572533251	25.27280672037864
39	22.036439691868086	26.378959048306406	26.825151341080815	24.759449918744693
40	22.609891054060558	24.90811522051856	28.637120174392056	23.844873551028826
41	20.845153403149073	26.80669092051709	28.076041221726346	24.272114454607497
42	21.00236155911999	27.11109147236583	28.282248047172263	23.604298921341915
43	21.092405206231373	26.958007452903832	28.548942208082323	23.400645132782465
44	21.30499462389348	26.06238247438371	28.450748481679504	24.181874420043304
45	21.13020979089646	24.68099313134884	28.530972756150607	25.657824321604096
46	21.57345627187879	26.515153746827114	27.894873796513142	24.016516184780954
47	20.411038938722207	26.614820379125977	29.547474211872604	23.426666470279216
48	20.387668831838333	26.29686909303365	28.687886330942312	24.627575744185705
49	21.357528267518987	25.023198267862668	29.533334315270597	24.085939149347748
50	20.45640444032031	24.845958591705575	29.063477334433102	25.634159633541014
51	20.456993602678725	24.772313296903462	28.205264165672457	26.565428934745363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	347.0
1	827.5
2	1308.0
3	3599.0
4	5890.0
5	3934.5
6	1979.0
7	1969.5
8	1960.0
9	1880.0
10	1800.0
11	1906.5
12	2013.0
13	2048.0
14	2083.0
15	2007.0
16	1931.0
17	1980.0
18	2029.0
19	2153.5
20	2278.0
21	2614.0
22	2950.0
23	3582.5
24	4215.0
25	5103.0
26	7495.0
27	8999.0
28	10568.5
29	12138.0
30	14990.5
31	17843.0
32	20375.0
33	22907.0
34	26302.5
35	29698.0
36	32186.0
37	34674.0
38	38616.0
39	42558.0
40	45398.0
41	48238.0
42	52029.0
43	55820.0
44	62200.0
45	68580.0
46	78371.0
47	88162.0
48	93712.5
49	99263.0
50	96973.0
51	94683.0
52	85000.0
53	75317.0
54	66969.0
55	58621.0
56	54436.5
57	50252.0
58	47245.0
59	44238.0
60	41218.0
61	38198.0
62	34424.0
63	30650.0
64	26658.0
65	22666.0
66	18485.0
67	14304.0
68	11521.5
69	8739.0
70	7528.5
71	6318.0
72	5036.0
73	3754.0
74	3017.0
75	1858.5
76	1437.0
77	1040.0
78	643.0
79	480.0
80	317.0
81	246.0
82	175.0
83	136.5
84	98.0
85	70.5
86	43.0
87	24.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1018395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.93688921209953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77260933012563	20.768760446491466
2	9.930262207582851	5.7470179469584775
3	3.6253623102151784	3.147201225732535
4	1.890824046428104	2.188582638042551
5	1.2801760780292026	1.852215667095556
6	0.9570461496217382	1.66163630414824
7	0.755931557306937	1.531201540800449
8	0.6748500004203039	1.5622447757558122
9	0.6296515753820189	1.6398142091148076
>10	8.25290864741147	53.49562569053816
>50	0.1903866948910155	3.329157745050395
>100	0.036573333988490164	1.8564093630288752
>500	0.002050841158229171	0.436284389168904
>1k	0.0013672274388194475	0.7838480580738074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3363	0.33022550189268407	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1899	0.18646988643895543	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1510	0.14827252686825837	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1153	0.1132173665424516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.945811792084604E-4	0.0	0.0	0.13157959337977895	0.0
2	2.945811792084604E-4	0.0	0.0	0.641401420863221	0.0
3	2.945811792084604E-4	0.0	0.0	0.9456055852591578	0.0
4	2.945811792084604E-4	0.0	0.0	1.2552104046072496	0.0
5	2.945811792084604E-4	0.0	0.0	2.1624222428429047	0.0
6	2.945811792084604E-4	0.0	0.0	2.8449668350689072	0.0
7	2.945811792084604E-4	0.0	0.0	3.5141570805041265	0.0
8	2.945811792084604E-4	0.0	0.0	4.726555020399747	0.0
9	2.945811792084604E-4	0.0	0.0	5.225575537978878	0.0
10	2.945811792084604E-4	0.0	0.0	6.226955159834838	0.0
11	2.945811792084604E-4	0.0	0.0	7.347738352996627	0.0
12	2.945811792084604E-4	0.0	0.0	8.426298243805203	0.0
13	2.945811792084604E-4	0.0	0.0	8.83429317700892	0.0
14	2.945811792084604E-4	0.0	0.0	9.00593581076105	0.0
15	2.945811792084604E-4	0.0	0.0	9.313282174401877	0.0
16	2.945811792084604E-4	0.0	0.0	9.871611702728313	0.0
17	2.945811792084604E-4	0.0	0.0	10.553468938869496	0.0
18	2.945811792084604E-4	0.0	0.0	11.279513351891948	0.0
19	2.945811792084604E-4	0.0	0.0	11.750646851172679	0.0
20	2.945811792084604E-4	0.0	0.0	12.25663912332641	0.0
21	2.945811792084604E-4	0.0	0.0	12.866029389382312	0.0
22	2.945811792084604E-4	0.0	0.0	13.52893523632775	0.0
23	2.945811792084604E-4	0.0	0.0	14.130960972903441	0.0
24	2.945811792084604E-4	0.0	0.0	14.601407116099352	0.0
25	2.945811792084604E-4	0.0	0.0	15.043671659817655	0.0
26	3.927749056112805E-4	0.0	0.0	15.458343766416764	0.0
27	3.927749056112805E-4	0.0	0.0	15.911016845133764	0.0
28	3.927749056112805E-4	0.0	0.0	16.3439529848438	0.0
29	3.927749056112805E-4	0.0	0.0	16.80546349893705	0.0
30	3.927749056112805E-4	0.0	0.0	17.30045807373367	0.0
31	3.927749056112805E-4	0.0	0.0	17.778170552683388	0.0
32	3.927749056112805E-4	0.0	0.0	18.221711614844928	0.0
33	4.909686320141006E-4	0.0	0.0	18.66083395931834	0.0
34	4.909686320141006E-4	0.0	0.0	19.115470912563396	0.0
35	4.909686320141006E-4	0.0	0.0	19.582185694156	0.0
36	4.909686320141006E-4	0.0	0.0	20.029752699100055	0.0
37	4.909686320141006E-4	0.0	0.0	20.485175202156334	0.0
38	4.909686320141006E-4	0.0	0.0	20.984490300914675	0.0
39	4.909686320141006E-4	0.0	0.0	21.567270067115413	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	20	7.032908E-4	45.0	1
TAACGCG	35	1.211738E-7	45.0	1
TGCGATA	20	7.032908E-4	45.0	1
TATCGCG	35	1.211738E-7	45.0	1
TATAGCG	75	0.0	42.0	1
TCACGAC	200	0.0	41.625	25
CGGTCTA	200	0.0	41.625	31
ACGGTCT	205	0.0	40.609756	30
ATAGACG	45	1.9279469E-8	40.000004	1
CACGACG	210	0.0	39.642857	26
GCGCGAC	105	0.0	38.57143	9
ACGGGTA	105	0.0	38.57143	5
CGTTTTT	1800	0.0	37.75	1
ACCGCTC	30	1.1398307E-4	37.499996	18
ATTCGCT	30	1.1398307E-4	37.499996	10
TCGTAAG	30	1.1398307E-4	37.499996	1
TATGCGG	145	0.0	37.241383	2
CGACGGT	225	0.0	37.0	28
TTTACGG	105	0.0	36.42857	2
AACGTAA	25	0.0021069958	36.0	41
>>END_MODULE