SRR3554425_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3549	0.3468184367860123	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2139	0.20902920154558477	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1821	0.1779533314700841	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	1426	0.13935280103038986	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1377	0.1345643807986303	Illumina PCR Primer Index 5 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1067	0.10427029361811078	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	35	1.2117562E-7	45.000004	1
AATTGCG	20	7.0329243E-4	45.0	1
TACGTTC	20	7.0329243E-4	45.0	34
TACGTCG	25	3.8905047E-5	45.0	1
CGACGGT	240	0.0	44.062504	28
CGGTCTA	250	0.0	42.300003	31
AGTTACG	50	1.0804797E-9	40.5	1
TCACGAC	280	0.0	40.178574	25
CACGACG	275	0.0	40.090908	26
CGTAAGG	130	0.0	39.80769	2
CTACGAA	120	0.0	39.375004	11
ACGCCGA	40	3.4577351E-7	39.375	12
TAGGTCG	40	3.4577351E-7	39.375	1
ATTATCG	40	3.4577351E-7	39.375	30
ACGGTCT	270	0.0	39.166664	30
TGTGACG	75	0.0	39.000004	1
CGCGTAA	35	6.2476174E-6	38.57143	31
TCGCGTA	35	6.2476174E-6	38.57143	30
CGTTTTT	2135	0.0	38.46604	1
CGCATGG	125	0.0	37.800003	2