##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15383337470268	33.0	31.0	34.0	30.0	34.0
2	32.264664781266916	34.0	31.0	34.0	30.0	34.0
3	32.267469427402666	34.0	31.0	34.0	30.0	34.0
4	35.83208476090147	37.0	35.0	37.0	35.0	37.0
5	35.91803104068985	37.0	35.0	37.0	35.0	37.0
6	35.911946815309655	37.0	35.0	37.0	35.0	37.0
7	36.13102583597022	37.0	36.0	37.0	35.0	37.0
8	35.97015934689857	37.0	36.0	37.0	35.0	37.0
9	37.76955971941812	39.0	38.0	39.0	35.0	39.0
10	37.57010735833605	39.0	37.0	39.0	35.0	39.0
11	37.569476068648356	39.0	37.0	39.0	35.0	39.0
12	37.4855761055876	39.0	37.0	39.0	35.0	39.0
13	37.46922023019597	39.0	37.0	39.0	35.0	39.0
14	38.70354890345177	40.0	38.0	41.0	35.0	41.0
15	38.832612464355584	40.0	38.0	41.0	35.0	41.0
16	38.862063203238144	40.0	38.0	41.0	35.0	41.0
17	38.81978047536309	40.0	38.0	41.0	35.0	41.0
18	38.78479862249854	40.0	38.0	41.0	35.0	41.0
19	38.770811549278704	40.0	38.0	41.0	35.0	41.0
20	38.72526683227434	40.0	38.0	41.0	35.0	41.0
21	38.65882799017299	40.0	38.0	41.0	35.0	41.0
22	38.66866965959218	40.0	38.0	41.0	35.0	41.0
23	38.64613769933021	40.0	38.0	41.0	35.0	41.0
24	38.59071906436223	40.0	38.0	41.0	35.0	41.0
25	38.48301967552101	40.0	38.0	41.0	34.0	41.0
26	38.479311092912944	40.0	38.0	41.0	34.0	41.0
27	38.44853230033753	40.0	38.0	41.0	34.0	41.0
28	38.389933763444226	40.0	38.0	41.0	34.0	41.0
29	38.38996210307417	40.0	38.0	41.0	34.0	41.0
30	38.241937375281196	40.0	37.0	41.0	34.0	41.0
31	38.23501566497476	40.0	38.0	41.0	34.0	41.0
32	38.14529825994672	40.0	37.0	41.0	34.0	41.0
33	38.09666061436409	40.0	37.0	41.0	34.0	41.0
34	37.98760776388593	40.0	37.0	41.0	34.0	41.0
35	37.918162966553375	40.0	37.0	41.0	33.0	41.0
36	37.86854320620892	40.0	37.0	41.0	33.0	41.0
37	37.83545913132193	40.0	37.0	41.0	33.0	41.0
38	37.75309146273534	40.0	37.0	41.0	33.0	41.0
39	37.668790835940904	40.0	37.0	41.0	33.0	41.0
40	37.58318951785495	40.0	36.0	41.0	33.0	41.0
41	37.48530834494607	40.0	36.0	41.0	33.0	41.0
42	37.500664515460734	40.0	36.0	41.0	33.0	41.0
43	37.474254912039655	40.0	36.0	41.0	33.0	41.0
44	37.45083269650602	40.0	36.0	41.0	33.0	41.0
45	37.37784446820196	40.0	36.0	41.0	33.0	41.0
46	37.26568989408796	40.0	35.0	41.0	33.0	41.0
47	37.23674242794405	40.0	35.0	41.0	33.0	41.0
48	37.17489265143623	39.0	35.0	41.0	32.0	41.0
49	37.095872968097396	39.0	35.0	41.0	32.0	41.0
50	37.01886051234142	39.0	35.0	41.0	32.0	41.0
51	36.249162026459445	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	20.0
10	32.0
11	31.0
12	25.0
13	38.0
14	50.0
15	59.0
16	91.0
17	162.0
18	232.0
19	344.0
20	537.0
21	858.0
22	1332.0
23	1900.0
24	3049.0
25	4549.0
26	6295.0
27	7489.0
28	8278.0
29	9452.0
30	11202.0
31	14163.0
32	18310.0
33	25077.0
34	44937.0
35	63962.0
36	65443.0
37	99564.0
38	190354.0
39	444772.0
40	686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.069009930597225	22.487984974132758	24.70150551841001	11.741499576860008
2	32.40626911703485	24.536158436121497	25.245137799007527	17.812434647836124
3	32.5290090315469	23.696132715464252	27.6039722388894	16.17088601409945
4	29.101477374225794	26.072948161930693	26.2779707261395	18.547603737704023
5	25.762091738313813	30.224019888556846	24.848578425528338	19.165309947601
6	27.637686626235464	34.341377227836944	23.160122818092802	14.860813327834792
7	82.12521816628913	5.4933929573087905	7.820956081391417	4.560432795010662
8	82.98107499056975	5.087647634813574	7.350322778612766	4.580954596003917
9	75.62655012889645	9.144319076870758	9.732708428205946	5.4964223660268425
10	33.2212777850527	38.09246928081837	15.760059102786862	12.926193831342067
11	30.293500843348298	25.256278205261008	25.50556922589812	18.944651725492573
12	28.891959558370843	22.252472877019688	27.871341988972954	20.98422557563652
13	26.251487830572014	22.89724831965539	30.18678747818337	20.664476371589227
14	21.776562539699913	27.02447566798462	28.49530246202978	22.70365933028568
15	19.825721048136327	26.889715841462248	32.62976130213759	20.654801808263837
16	23.216411186531445	25.655866987458246	30.84543956720499	20.282282258805317
17	22.930669538415835	25.482506630496175	28.663874398760093	22.922949432327897
18	23.004938913439045	25.783199876478303	29.799511776582083	21.41234943350057
19	23.796005480298092	27.10392435468708	27.726809876263314	21.37326028875151
20	25.204778257054123	26.207512542729322	28.12776677852677	20.459942421689785
21	24.74186506036341	26.37266417929409	28.4748783838984	20.4105923764441
22	23.447525754860248	25.434329259592964	27.627132557153217	23.491012428393574
23	22.22853077586089	26.784761487811025	28.438720925005523	22.547986811322563
24	22.11575859325986	25.38136346845799	28.85717021954418	23.645707718737967
25	22.422803825263703	27.21542614008377	26.792872485346457	23.56889754930607
26	21.667112934402553	27.817301246357378	27.407549286525384	23.10803653271468
27	22.26429734330628	27.543188618804614	27.341293186175736	22.851220851713375
28	20.33778884434898	27.411653646724037	28.68459164547709	23.565965863449893
29	21.907706620333002	25.60211941342829	28.29008445209723	24.200089514141474
30	22.58160347580675	26.560878411260802	27.233016255220843	23.624501857711603
31	21.68958919263326	28.44311845378979	25.625572900277728	24.241719453299222
32	22.09728897236593	28.323701116581418	26.259110213798074	23.319899697254574
33	22.601734385352515	27.812805994711237	25.95128319889925	23.634176421036994
34	21.265765140691602	26.860789874347944	26.968969082440964	24.90447590251949
35	20.67200103195342	27.702476883657024	27.073727990368436	24.55179409402112
36	21.270944452370856	28.643938934938074	26.01939603362448	24.06572057906659
37	21.270749006647108	29.131576015682565	26.592638341369412	23.005036636300915
38	21.056345047698528	28.10499735171044	26.831473015786152	24.00718458480488
39	22.592743882060233	25.976984311571755	27.226957437784737	24.20331436858327
40	23.00552525061028	26.061612309953464	28.886487078105976	22.04637536133028
41	19.927255101622006	27.898606667435423	27.313833061989516	24.860305168953055
42	20.604474534399426	27.879159817922766	27.03913409726552	24.47723155041229
43	21.252670277200668	26.59244289564566	27.704138172308856	24.450748654844805
44	21.71773337685258	26.218359780397183	27.477616578488075	24.586290264262164
45	21.557467883381445	25.33142708604107	27.569085177200865	25.54201985337662
46	21.56215858075133	27.411165032414676	26.7516334376362	24.275042949197793
47	20.34707251622688	27.269857774146832	28.481914429953232	23.90115527967306
48	20.336616170006508	26.01314177046463	28.22275340026698	25.427488659261883
49	21.30847003132995	24.919818391833495	28.910917793574136	24.86079378326242
50	20.191204551540014	24.66270954224657	29.650093520778814	25.4959923854346
51	20.130714100040848	25.170184363951208	27.548270207621993	27.150831328385948
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	443.0
1	859.0
2	1275.0
3	3693.5
4	6112.0
5	4156.0
6	2200.0
7	2095.0
8	1990.0
9	2013.0
10	2036.0
11	2030.0
12	2024.0
13	2028.5
14	2033.0
15	1937.0
16	1841.0
17	1876.0
18	1911.0
19	2099.5
20	2288.0
21	2556.5
22	2825.0
23	3220.0
24	3615.0
25	4938.0
26	7761.5
27	9262.0
28	11075.5
29	12889.0
30	14829.0
31	16769.0
32	18675.0
33	20581.0
34	23716.0
35	26851.0
36	29885.5
37	32920.0
38	36200.0
39	39480.0
40	43709.5
41	47939.0
42	51610.5
43	55282.0
44	62223.5
45	69165.0
46	77458.5
47	85752.0
48	94735.5
49	103719.0
50	101040.0
51	98361.0
52	86503.0
53	74645.0
54	67236.0
55	59827.0
56	55387.5
57	50948.0
58	47570.0
59	44192.0
60	43377.0
61	42562.0
62	38288.5
63	34015.0
64	28895.5
65	23776.0
66	19165.0
67	14554.0
68	11469.0
69	8384.0
70	7329.5
71	6275.0
72	4925.0
73	3575.0
74	2887.5
75	1729.5
76	1259.0
77	1005.0
78	751.0
79	519.0
80	287.0
81	240.0
82	193.0
83	107.5
84	22.0
85	14.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1023302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.439410929291558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47217185889865	21.040986373630655
2	10.627308322406801	6.257233935512279
3	3.7915603542215903	3.3486390999341906
4	2.0180752643583846	2.376437879747407
5	1.291140434831212	1.9005206914210122
6	0.9360621902554869	1.6534271684582387
7	0.7642351724860621	1.574904330260464
8	0.6449917685262537	1.5190542175723907
9	0.5627216889133576	1.4909575534870771
>10	7.620399796140326	50.644145074022006
>50	0.2174015771741691	3.906472212337527
>100	0.048906891186393436	2.527226030458306
>500	0.003014808360800747	0.6378467227919858
>1k	0.002009872240533831	1.1221487103664205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3549	0.3468184367860123	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2139	0.20902920154558477	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1821	0.1779533314700841	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	1426	0.13935280103038986	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1377	0.1345643807986303	Illumina PCR Primer Index 5 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1067	0.10427029361811078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9089144749057463E-4	0.0	0.0	0.14472755843338525	0.0
2	3.9089144749057463E-4	0.0	0.0	0.6835714187991424	0.0
3	4.886143093632183E-4	0.0	0.0	1.0372304559162397	0.0
4	4.886143093632183E-4	0.0	0.0	1.381312652569818	0.0
5	4.886143093632183E-4	0.0	0.0	2.37388376061026	0.0
6	4.886143093632183E-4	0.0	0.0	3.1575233899669892	0.0
7	4.886143093632183E-4	0.0	0.0	3.890249408288071	0.0
8	4.886143093632183E-4	0.0	0.0	5.209410320706888	0.0
9	4.886143093632183E-4	0.0	0.0	5.708774144876097	0.0
10	4.886143093632183E-4	0.0	0.0	6.754506489775257	0.0
11	4.886143093632183E-4	0.0	0.0	8.067217693310479	0.0
12	4.886143093632183E-4	0.0	0.0	9.216047657485278	0.0
13	4.886143093632183E-4	0.0	0.0	9.686974128849547	0.0
14	4.886143093632183E-4	0.0	0.0	9.880954009666745	0.0
15	4.886143093632183E-4	0.0	0.0	10.218488774574856	0.0
16	4.886143093632183E-4	0.0	0.0	10.845967270659102	0.0
17	4.886143093632183E-4	0.0	0.0	11.638499680446241	0.0
18	4.886143093632183E-4	0.0	0.0	12.491033927423185	0.0
19	4.886143093632183E-4	0.0	0.0	12.99469755751479	0.0
20	4.886143093632183E-4	0.0	0.0	13.577516705723237	0.0
21	4.886143093632183E-4	0.0	0.0	14.2674401105441	0.0
22	4.886143093632183E-4	0.0	0.0	15.02743080732765	0.0
23	4.886143093632183E-4	0.0	0.0	15.736214724489935	0.0
24	4.886143093632183E-4	0.0	0.0	16.297241674500782	0.0
25	4.886143093632183E-4	0.0	0.0	16.801589364625496	0.0
26	4.886143093632183E-4	0.0	0.0	17.28893327678437	0.0
27	4.886143093632183E-4	0.0	0.0	17.73992428432662	0.0
28	4.886143093632183E-4	0.0	0.0	18.231763448131638	0.0
29	6.840600331085056E-4	0.0	0.0	18.718227854533655	0.0
30	6.840600331085056E-4	0.0	0.0	19.27133925273282	0.0
31	6.840600331085056E-4	0.0	0.0	19.80207211556315	0.0
32	7.817828949811493E-4	0.0	0.0	20.306419805687863	0.0
33	7.817828949811493E-4	0.0	0.0	20.782427865869508	0.0
34	7.817828949811493E-4	0.0	0.0	21.284234761585534	0.0
35	7.817828949811493E-4	0.0	0.0	21.818387924581405	0.0
36	7.817828949811493E-4	0.0	0.0	22.330455720794056	0.0
37	8.795057568537929E-4	0.0	0.0	22.83030815927263	0.0
38	8.795057568537929E-4	0.0	0.0	23.32595851469068	0.0
39	8.795057568537929E-4	0.0	0.0	23.83831947948895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	35	1.2117562E-7	45.000004	1
AATTGCG	20	7.0329243E-4	45.0	1
TACGTTC	20	7.0329243E-4	45.0	34
TACGTCG	25	3.8905047E-5	45.0	1
CGACGGT	240	0.0	44.062504	28
CGGTCTA	250	0.0	42.300003	31
AGTTACG	50	1.0804797E-9	40.5	1
TCACGAC	280	0.0	40.178574	25
CACGACG	275	0.0	40.090908	26
CGTAAGG	130	0.0	39.80769	2
CTACGAA	120	0.0	39.375004	11
ACGCCGA	40	3.4577351E-7	39.375	12
TAGGTCG	40	3.4577351E-7	39.375	1
ATTATCG	40	3.4577351E-7	39.375	30
ACGGTCT	270	0.0	39.166664	30
TGTGACG	75	0.0	39.000004	1
CGCGTAA	35	6.2476174E-6	38.57143	31
TCGCGTA	35	6.2476174E-6	38.57143	30
CGTTTTT	2135	0.0	38.46604	1
CGCATGG	125	0.0	37.800003	2
>>END_MODULE