FastQCFastQC Report
Sat 18 Jun 2016
SRR3554423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171726
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC171671.4651036163744766No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC159031.35722856708821No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG138351.18073679341416No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72720.6206229101342805No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC44880.3830247003138959TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT44570.3803790305924764No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT34660.2958029436916139No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT30640.26149458149772214No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC24300.20738636848546504No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA23680.202095029042626No Hit
CGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTG19950.17026164820102993No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC16390.13987911849698648TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT16110.13748948132925276TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT13780.11760428632632544No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT13630.11632412355789665No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC12460.10633885396415202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG207.0333434E-445.02
CCGCGTA207.0333434E-445.040
AATGTCG207.0333434E-445.01
CGATCGA1000.042.7541
CGTTTTT44200.041.640271
CCGATGA26950.041.57699618
CGATGAA28550.040.98073619
CTATCGA2200.040.90909241
CCGATCG1050.040.71428740
TACGGCT18850.040.5835577
ACGGCTG18950.040.250668
CGTAAGG1800.040.02
ATCGAAT4500.040.043
GATGAAT31300.039.89616820
AACGGGC1250.039.6000024
ACTAACG800.039.37543
ATGAATG31600.039.0189921
TGAATGA31300.038.96166222
CGGCTGT19700.038.604069
CAATGCG356.248394E-638.5714261
Produced by FastQC (version 0.11.5)