##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1171726
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.087380496805565	33.0	31.0	34.0	30.0	34.0
2	32.20665240849823	33.0	31.0	34.0	30.0	34.0
3	32.21469865821873	34.0	31.0	34.0	30.0	34.0
4	35.821763791193504	37.0	35.0	37.0	35.0	37.0
5	35.86256087173964	37.0	35.0	37.0	35.0	37.0
6	35.84695312726696	37.0	35.0	37.0	35.0	37.0
7	36.04000764683894	37.0	35.0	37.0	35.0	37.0
8	35.73134504141753	37.0	35.0	37.0	35.0	37.0
9	37.59240897615996	39.0	37.0	39.0	35.0	39.0
10	37.48313598913057	39.0	37.0	39.0	35.0	39.0
11	37.405798795964245	39.0	37.0	39.0	35.0	39.0
12	37.32467061411968	39.0	37.0	39.0	34.0	39.0
13	37.29205035989643	39.0	37.0	39.0	34.0	39.0
14	38.52918856456202	40.0	38.0	41.0	34.0	41.0
15	38.575449379803814	40.0	38.0	41.0	34.0	41.0
16	38.702166718157656	40.0	38.0	41.0	35.0	41.0
17	38.57360253164989	40.0	38.0	41.0	34.0	41.0
18	38.54995451154963	40.0	38.0	41.0	34.0	41.0
19	38.47559327009898	40.0	37.0	41.0	34.0	41.0
20	38.21825921759866	40.0	37.0	41.0	34.0	41.0
21	38.30336870565303	40.0	37.0	41.0	34.0	41.0
22	38.28455031295712	40.0	37.0	41.0	34.0	41.0
23	38.134187514828554	40.0	37.0	41.0	34.0	41.0
24	38.149925835903616	40.0	37.0	41.0	34.0	41.0
25	38.167214860812166	40.0	37.0	41.0	34.0	41.0
26	38.047291772991294	40.0	37.0	41.0	34.0	41.0
27	37.934769732855635	40.0	37.0	41.0	33.0	41.0
28	37.87659316256531	40.0	36.0	41.0	33.0	41.0
29	37.835703056858	40.0	36.0	41.0	33.0	41.0
30	37.718205450762376	40.0	36.0	41.0	33.0	41.0
31	37.722657856870974	40.0	36.0	41.0	33.0	41.0
32	37.42219000005121	40.0	36.0	41.0	33.0	41.0
33	37.20893707231895	40.0	36.0	41.0	32.0	41.0
34	37.0469102844863	40.0	36.0	41.0	31.0	41.0
35	36.959261806941214	40.0	36.0	41.0	31.0	41.0
36	36.95595386634759	40.0	36.0	41.0	31.0	41.0
37	36.782458526993516	40.0	36.0	41.0	31.0	41.0
38	36.77684202620749	40.0	35.0	41.0	30.0	41.0
39	36.622951952930975	40.0	35.0	41.0	30.0	41.0
40	36.566648687491785	40.0	35.0	41.0	30.0	41.0
41	36.545324589537145	40.0	35.0	41.0	30.0	41.0
42	36.4239446764858	40.0	35.0	41.0	30.0	41.0
43	36.416004253554156	40.0	35.0	41.0	30.0	41.0
44	36.46305706282868	40.0	35.0	41.0	30.0	41.0
45	36.38158067671111	39.0	35.0	41.0	30.0	41.0
46	36.26020076365976	39.0	35.0	41.0	29.0	41.0
47	36.081337275096736	39.0	35.0	41.0	29.0	41.0
48	36.078407409240725	39.0	35.0	41.0	29.0	41.0
49	36.04752049540592	39.0	35.0	41.0	29.0	41.0
50	35.941307950834926	39.0	35.0	41.0	28.0	41.0
51	35.17090428991078	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	21.0
10	32.0
11	23.0
12	26.0
13	33.0
14	54.0
15	71.0
16	79.0
17	170.0
18	309.0
19	608.0
20	1192.0
21	2647.0
22	4982.0
23	7439.0
24	9345.0
25	11663.0
26	13713.0
27	13989.0
28	13456.0
29	13360.0
30	15246.0
31	18452.0
32	23863.0
33	31970.0
34	54740.0
35	74925.0
36	73028.0
37	108179.0
38	201196.0
39	476116.0
40	792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72718024521091	18.95528476794063	23.149951439158983	13.167583547689476
2	30.98557171215796	30.478029846568226	23.32669924538672	15.209699195887094
3	28.709612998260685	29.86884305716524	26.69514886586113	14.726395078712942
4	25.441101417908285	23.58588953390127	34.70333508004431	16.269673968146137
5	30.33772400714843	27.05035136200784	25.009089155655843	17.602835475187888
6	24.664469338394813	37.51662077994343	24.290491121644482	13.528418760017274
7	74.69638806342097	4.770569228642191	16.198155541483246	4.334887166453591
8	74.97315925395527	10.521316416978031	9.97579638925824	4.529727939808454
9	68.98455782324537	7.02749618938216	11.303495868488026	12.684450118884449
10	38.496798739637086	30.343954132621448	17.86262317299437	13.2966239547471
11	29.851176810960922	22.062239806917315	29.612554470925794	18.474028911195962
12	24.469287188301703	20.53620044276563	31.376533421636115	23.617978947296553
13	25.442978989968644	21.317441108245443	34.97618043808877	18.263399463697144
14	20.48994389473307	28.873559176804132	29.63636549841857	21.000131430044224
15	18.341660080940425	24.118010524644838	35.939545593423716	21.600783800991017
16	21.342532298506647	25.29243184840142	29.558531602098103	23.806504250993832
17	21.527729179006013	26.646929401583648	30.991631149261856	20.83371027014848
18	21.351066716962837	24.833024102904606	30.236591148442553	23.579318031690004
19	21.60991562873914	25.7956211605785	28.875436748864498	23.719026461817865
20	25.01728219737379	25.499562184333197	31.33027687360356	18.152878744689456
21	23.124177495421286	29.090333405591412	29.38460015396091	18.4008889450264
22	20.99466940223226	23.184174457168314	32.489165555769866	23.33199058482956
23	22.172419149186755	27.190486513058516	30.20919566519818	20.42789867255655
24	21.531825699864985	26.151079689278895	29.283808671993285	23.03328593886284
25	20.3083314699853	30.96773477758452	27.41835548583884	21.305578266591336
26	19.999385521871154	25.78520917006194	31.297334018362655	22.918071289704248
27	24.4309676494334	25.58012709455965	28.258142261928132	21.73076299407882
28	19.00341888803355	28.168530868138113	30.580528212227094	22.247522031601243
29	22.175662228200107	25.11252630734489	30.1567089916926	22.555102472762403
30	22.833751235357074	27.63043578447521	29.008061611673718	20.527751368494
31	22.59581164879844	24.353901850773987	26.36213585770052	26.688150642727056
32	26.275255477816483	26.577117858612	26.720410744491456	20.42721591908006
33	24.067998832491554	24.228787276206212	27.29827621816022	24.404937673142012
34	21.684762478599946	25.975099980712212	27.579912027214554	24.760225513473287
35	24.346135529978852	24.159317109972807	28.537303089630168	22.95724427041817
36	20.365682762010913	30.386114159795035	26.869336346552007	22.378866731642038
37	24.003563973147305	25.07070765690955	28.502994727436278	22.422733642506863
38	20.9358672590691	25.548720434640863	28.259251736327435	25.256160569962603
39	23.405045206814563	26.14595903820518	27.10992160283206	23.3390741521482
40	21.134377832360123	23.733108252270583	32.489165555769866	22.64334835959943
41	19.80189907879487	27.04087815752147	27.57214570641942	25.58507705726424
42	21.424548059870652	25.380677735238443	31.389164360951284	21.805609843939624
43	22.347716104276937	27.240754237765486	27.62190136601902	22.78962829193856
44	22.69412814941377	24.389405031551746	29.432051520577335	23.484415298457147
45	21.544200606626465	22.82299786810227	28.775413364557927	26.85738816071334
46	24.90129945055414	26.11019982487373	26.889648262477746	22.09885246209438
47	19.931878271882674	25.93353736283056	32.43736163574078	21.697222729545988
48	21.65753768372469	25.397661227966267	28.213592597586807	24.731208490722235
49	21.50306470966762	23.195013168607677	32.41525749193924	22.88666462978546
50	21.311808392064357	23.867183966217357	30.429981070659863	24.391026571058422
51	20.36542672945723	23.364336030778528	28.965560207761882	27.30467703200236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	807.0
2	1247.0
3	6942.5
4	12638.0
5	8585.5
6	4533.0
7	4335.5
8	4138.0
9	4127.0
10	4116.0
11	4192.5
12	4269.0
13	4142.0
14	4015.0
15	3922.5
16	3830.0
17	3626.5
18	3423.0
19	3426.5
20	3430.0
21	3628.0
22	3826.0
23	4214.0
24	4602.0
25	5937.5
26	8661.0
27	10049.0
28	11882.5
29	13716.0
30	16553.0
31	19390.0
32	20864.0
33	22338.0
34	26279.5
35	30221.0
36	33507.0
37	36793.0
38	39411.0
39	42029.0
40	46073.0
41	50117.0
42	54667.0
43	59217.0
44	65461.5
45	71706.0
46	110722.0
47	149738.0
48	131465.5
49	113193.0
50	111425.5
51	109658.0
52	96539.0
53	83420.0
54	75621.0
55	67822.0
56	60995.0
57	54168.0
58	49786.5
59	45405.0
60	42723.0
61	40041.0
62	35693.5
63	31346.0
64	25960.5
65	20575.0
66	17179.5
67	13784.0
68	11448.0
69	9112.0
70	7735.5
71	6359.0
72	5222.5
73	4086.0
74	3437.0
75	2149.0
76	1510.0
77	1170.5
78	831.0
79	579.5
80	328.0
81	227.0
82	126.0
83	121.5
84	117.0
85	69.0
86	21.0
87	15.5
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1171726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.850232277158995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82279623450462	21.009545864376776
2	9.24448799034933	5.334112516099699
3	3.4801478918729263	3.0120922511819734
4	1.9077021964096164	2.201506059282553
5	1.2638609725047985	1.8231341311399745
6	0.9992768495825289	1.729762153178618
7	0.8362269783944143	1.6887739794173966
8	0.7035399481095828	1.6237832735377478
9	0.6385575037527333	1.6580279075030262
>10	7.940885732068118	48.08843448973795
>50	0.12025158826720926	2.22798133914181
>100	0.03422959951637115	1.7741225205642859
>500	0.0032741356053662177	0.642754456201967
>1k	0.0035717842967631468	2.533663357413365
>5k	2.976486913969289E-4	0.6244636480294862
>10k+	8.929460741907867E-4	4.027842053193488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	17167	1.4651036163744766	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	15903	1.35722856708821	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	13835	1.18073679341416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7272	0.6206229101342805	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	4488	0.3830247003138959	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT	4457	0.3803790305924764	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	3466	0.2958029436916139	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	3064	0.26149458149772214	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	2430	0.20738636848546504	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	2368	0.202095029042626	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTG	1995	0.17026164820102993	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1639	0.13987911849698648	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	1611	0.13748948132925276	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	1378	0.11760428632632544	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	1363	0.11632412355789665	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	1246	0.10633885396415202	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7068836912383954E-4	0.0	0.0	0.1408179045271676	0.0
2	1.7068836912383954E-4	0.0	0.0	0.7630623541681246	0.0
3	2.560325536857593E-4	0.0	0.0	1.1209105200362541	0.0
4	2.560325536857593E-4	0.0	0.0	1.7022751052720517	0.0
5	2.560325536857593E-4	0.0	0.0	3.7914153991632857	0.0
6	2.560325536857593E-4	0.0	0.0	4.66115798403381	0.0
7	2.560325536857593E-4	0.0	0.0	5.59738368867807	0.0
8	2.560325536857593E-4	0.0	0.0	6.94437095361885	0.0
9	2.560325536857593E-4	0.0	0.0	7.500473660224318	0.0
10	2.560325536857593E-4	0.0	0.0	9.563498633639606	0.0
11	2.560325536857593E-4	0.0	0.0	10.521060384424345	0.0
12	2.560325536857593E-4	0.0	0.0	12.863758250649042	0.0
13	2.560325536857593E-4	0.0	0.0	13.333236609924164	0.0
14	2.560325536857593E-4	0.0	0.0	13.562727122211166	0.0
15	2.560325536857593E-4	0.0	0.0	14.024524504875714	0.0
16	2.560325536857593E-4	0.0	0.0	14.499806268701045	0.0
17	2.560325536857593E-4	0.0	0.0	15.08424324458107	0.0
18	2.560325536857593E-4	0.0	0.0	15.706743726775715	0.0
19	2.560325536857593E-4	0.0	0.0	16.50991784768794	0.0
20	3.413767382476791E-4	0.0	0.0	16.985455644066956	0.0
21	3.413767382476791E-4	0.0	0.0	17.46440720782845	0.0
22	3.413767382476791E-4	0.0	0.0	18.021619388833226	0.0
23	3.413767382476791E-4	0.0	0.0	18.526344896332418	0.0
24	3.413767382476791E-4	0.0	0.0	18.941629698410722	0.0
25	3.413767382476791E-4	0.0	0.0	19.307244185073984	0.0
26	3.413767382476791E-4	0.0	0.0	19.67516296472042	0.0
27	3.413767382476791E-4	0.0	0.0	20.07431771591652	0.0
28	3.413767382476791E-4	0.0	0.0	20.442919249039452	0.0
29	3.413767382476791E-4	0.0	0.0	20.834649056178662	0.0
30	3.413767382476791E-4	0.0	0.0	21.274683671779922	0.0
31	3.413767382476791E-4	0.0	0.0	21.67435048808339	0.0
32	3.413767382476791E-4	0.0	0.0	22.064544099900488	0.0
33	3.413767382476791E-4	0.0	0.0	22.45217738618073	0.0
34	3.413767382476791E-4	0.0	0.0	22.849539909501026	0.0
35	3.413767382476791E-4	0.0	0.0	23.251254986233985	0.0
36	3.413767382476791E-4	0.0	0.0	23.646995970047605	0.0
37	3.413767382476791E-4	0.0	0.0	24.042992986414912	0.0
38	3.413767382476791E-4	0.0	0.0	24.453925235080558	0.0
39	3.413767382476791E-4	0.0	0.0	24.918112254912838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCG	20	7.0333434E-4	45.0	2
CCGCGTA	20	7.0333434E-4	45.0	40
AATGTCG	20	7.0333434E-4	45.0	1
CGATCGA	100	0.0	42.75	41
CGTTTTT	4420	0.0	41.64027	1
CCGATGA	2695	0.0	41.576996	18
CGATGAA	2855	0.0	40.980736	19
CTATCGA	220	0.0	40.909092	41
CCGATCG	105	0.0	40.714287	40
TACGGCT	1885	0.0	40.583557	7
ACGGCTG	1895	0.0	40.25066	8
CGTAAGG	180	0.0	40.0	2
ATCGAAT	450	0.0	40.0	43
GATGAAT	3130	0.0	39.896168	20
AACGGGC	125	0.0	39.600002	4
ACTAACG	80	0.0	39.375	43
ATGAATG	3160	0.0	39.01899	21
TGAATGA	3130	0.0	38.961662	22
CGGCTGT	1970	0.0	38.60406	9
CAATGCG	35	6.248394E-6	38.571426	1
>>END_MODULE