SRR3554421_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295600
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7338	2.482408660351827	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1390	0.4702300405953992	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1121	0.37922868741542626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	1033	0.34945872801082545	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	1026	0.3470906630581867	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	644	0.21786197564276047	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	504	0.17050067658998647	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	446	0.15087956698240867	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	329	0.11129905277401893	TruSeq Adapter, Index 16 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.024775E-4	45.000004	1
CGGTTCG	20	7.024775E-4	45.000004	30
TTGTCCG	20	7.024775E-4	45.000004	25
ATTCGAC	20	7.024775E-4	45.000004	42
CAATTAG	20	7.024775E-4	45.000004	36
AGTATCG	20	7.024775E-4	45.000004	28
GACCATA	20	7.024775E-4	45.000004	9
TATTGCG	20	7.024775E-4	45.000004	1
TCAGATC	20	7.024775E-4	45.000004	17
GCGATAT	20	7.024775E-4	45.000004	9
GCGATAC	20	7.024775E-4	45.000004	9
CGGTCTA	20	7.024775E-4	45.000004	31
ACCCGGG	20	7.024775E-4	45.000004	3
GTGCGGT	20	7.024775E-4	45.000004	12
TCGAATG	20	7.024775E-4	45.000004	44
CGAAAGG	50	2.1827873E-11	45.0	2
GCGCGAC	25	3.8837527E-5	45.0	9
GGCCGAT	25	3.8837527E-5	45.0	8
TTCACGG	25	3.8837527E-5	45.0	2
GCGTATG	25	3.8837527E-5	45.0	1