##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295600
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.844945872801084	33.0	31.0	34.0	30.0	34.0
2	31.93955345060893	33.0	31.0	34.0	30.0	34.0
3	31.954411366711774	33.0	31.0	34.0	30.0	34.0
4	35.63786535859269	37.0	35.0	37.0	33.0	37.0
5	35.13610960757781	37.0	35.0	37.0	33.0	37.0
6	35.32831867388363	37.0	35.0	37.0	33.0	37.0
7	35.84408660351827	37.0	35.0	37.0	35.0	37.0
8	35.63689106901218	37.0	35.0	37.0	34.0	37.0
9	37.43624154262517	39.0	37.0	39.0	34.0	39.0
10	37.09841339648173	39.0	37.0	39.0	33.0	39.0
11	37.199316644113665	39.0	37.0	39.0	34.0	39.0
12	37.163964817320704	39.0	37.0	39.0	34.0	39.0
13	37.1496549391069	39.0	37.0	39.0	33.0	39.0
14	38.22404939106901	40.0	37.0	41.0	33.0	41.0
15	38.37707713125846	40.0	38.0	41.0	34.0	41.0
16	38.18795331529093	40.0	37.0	41.0	33.0	41.0
17	38.219242219215154	40.0	37.0	41.0	34.0	41.0
18	38.037655615696885	39.0	37.0	41.0	34.0	41.0
19	37.73940798376184	39.0	37.0	41.0	34.0	41.0
20	37.60375845737483	39.0	35.0	41.0	34.0	41.0
21	37.50689106901218	39.0	35.0	41.0	33.0	41.0
22	37.60743910690122	39.0	35.0	41.0	33.0	41.0
23	37.612577807848446	39.0	35.0	41.0	34.0	41.0
24	37.514692151556154	39.0	35.0	41.0	33.0	41.0
25	37.26596075778078	39.0	35.0	41.0	33.0	41.0
26	37.35283152909337	39.0	35.0	41.0	33.0	41.0
27	37.349208389715834	39.0	35.0	41.0	33.0	41.0
28	37.2156799729364	39.0	35.0	41.0	33.0	41.0
29	37.191204330175914	39.0	35.0	41.0	33.0	41.0
30	36.85131596752368	39.0	35.0	41.0	32.0	41.0
31	36.69695534506089	39.0	35.0	41.0	31.0	41.0
32	36.35877537212449	39.0	35.0	41.0	30.0	41.0
33	35.970321380243576	39.0	35.0	41.0	28.0	41.0
34	35.62468200270636	39.0	35.0	41.0	24.0	41.0
35	35.40472259810555	39.0	35.0	41.0	23.0	41.0
36	35.19085250338295	39.0	35.0	41.0	22.0	41.0
37	35.14735791610284	39.0	35.0	41.0	21.0	41.0
38	35.02299052774019	39.0	35.0	41.0	21.0	41.0
39	34.92996955345061	39.0	35.0	41.0	21.0	41.0
40	34.83483085250338	39.0	35.0	41.0	20.0	41.0
41	34.74331867388363	38.0	35.0	41.0	20.0	41.0
42	34.71343707713126	39.0	35.0	41.0	18.0	41.0
43	34.597997293640056	38.0	35.0	41.0	18.0	41.0
44	34.534871447902574	38.0	35.0	41.0	18.0	41.0
45	34.42698917456022	38.0	34.0	41.0	20.0	41.0
46	34.281427604871446	38.0	34.0	40.0	18.0	41.0
47	34.21929296346414	38.0	34.0	40.0	18.0	41.0
48	34.22597428958051	38.0	34.0	40.0	18.0	41.0
49	34.23951623815967	37.0	34.0	40.0	19.0	41.0
50	34.0964648173207	37.0	34.0	40.0	18.0	41.0
51	33.32618403247632	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	10.0
11	11.0
12	3.0
13	5.0
14	14.0
15	23.0
16	29.0
17	60.0
18	132.0
19	232.0
20	416.0
21	723.0
22	1126.0
23	1743.0
24	2547.0
25	4306.0
26	6291.0
27	7270.0
28	6940.0
29	6187.0
30	5995.0
31	6197.0
32	7439.0
33	9779.0
34	17066.0
35	23670.0
36	22253.0
37	29729.0
38	51750.0
39	83585.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.185047361299056	15.65764546684709	18.756427604871448	23.400879566982407
2	39.204668470906626	22.940121786197565	21.44824086603518	16.406968876860624
3	28.717523680649528	23.000338294993234	34.26894451962111	14.01319350473613
4	25.351826792963468	21.607577807848443	35.869418132611635	17.171177266576453
5	22.904600811907983	26.900202976995942	32.65460081190798	17.540595399188092
6	21.972259810554803	31.906968876860624	32.280446549391066	13.840324763193504
7	69.0118403247632	5.495263870094723	21.3521650879567	4.140730717185385
8	69.17320703653586	6.714140730717186	19.22361299052774	4.889039242219216
9	63.18098782138024	7.078484438430312	22.302435723951287	7.438092016238159
10	32.97767253044655	26.89952638700947	27.314952638700944	12.807848443843032
11	28.60893098782138	22.37618403247632	33.10757780784844	15.907307171853857
12	24.134303112313937	20.946887686062247	36.48240866035183	18.43640054127199
13	23.221583220568334	22.018944519621108	38.68403247631935	16.075439783491206
14	18.290933694181327	26.0	36.856224627875505	18.852841677943168
15	17.410013531799727	21.519282814614343	42.10453315290933	18.96617050067659
16	20.33761840324763	22.10656292286874	38.42523680649526	19.130581867388365
17	20.773342354533153	22.280108254397835	36.388700947225985	20.557848443843028
18	22.834573748308525	19.718200270635993	38.059878213802435	19.387347767253043
19	21.492895805142084	22.486468200270636	35.570365358592696	20.45027063599459
20	23.161366711772665	22.528416779431666	36.418809201623816	17.891407307171853
21	21.073071718538568	25.383964817320702	36.0426251691475	17.500338294993234
22	20.530784844384304	22.385656292286875	35.820365358592696	21.26319350473613
23	19.46278755074425	24.298376184032477	36.385656292286875	19.8531799729364
24	19.646143437077132	21.990527740189446	37.6904600811908	20.672868741542626
25	18.335926928281463	24.645466847090663	36.58220568335588	20.43640054127199
26	19.670500676589985	22.17083897158322	37.504736129905275	20.653924221921514
27	20.391745602165088	22.316305818673886	36.12415426251692	21.167794316644116
28	17.166779431664413	24.132949932341003	38.3958051420839	20.30446549391069
29	20.862313937753722	22.40832205683356	36.79127198917456	19.93809201623816
30	20.171853856562922	22.72462787550744	36.85081190798376	20.252706359945876
31	21.945872801082544	22.185047361299056	34.3362652232747	21.532814614343707
32	22.62381596752368	23.429296346414073	34.74424898511502	19.202638700947226
33	21.263531799729364	22.215832205683355	34.3362652232747	22.184370771312583
34	21.053112313937753	22.31935047361299	35.42895805142084	21.198579161028416
35	20.644790257104194	22.94722598105548	34.134979702300406	22.273004059539918
36	21.370094722598104	26.143098782138026	33.049052774018946	19.437753721244928
37	20.848782138024358	25.22530446549391	32.54330175913396	21.382611637347768
38	20.65324763193505	26.518944519621108	32.27334235453315	20.55446549391069
39	22.44384303112314	23.250338294993234	32.250338294993234	22.055480378890394
40	23.095399188092017	22.000338294993234	33.0426251691475	21.861637347767253
41	19.12043301759134	23.277401894451963	32.35148849797023	25.25067658998647
42	22.00981055480379	22.566644113667117	33.015899864682005	22.40764546684709
43	22.18809201623816	22.16576454668471	33.06935047361299	22.57679296346414
44	21.460081190798377	23.019621109607577	32.903924221921514	22.61637347767253
45	21.53382949932341	22.147158322056836	32.07848443843031	24.240527740189446
46	21.494587280108256	23.175575101488498	32.81393775372125	22.515899864682
47	18.628890392422193	24.043978349120433	35.39242219215156	21.934709066305818
48	20.08152909336942	23.363328822733422	33.70906630581867	22.846075778078482
49	21.41441136671177	20.906292286874155	35.800405953991884	21.878890392422193
50	19.914411366711775	22.717861975642762	34.338633288227335	23.02909336941813
51	19.747970230040597	21.00913396481732	32.58389715832205	26.658998646820027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	435.0
2	679.0
3	6513.0
4	12347.0
5	7912.5
6	3478.0
7	3304.0
8	3130.0
9	3115.0
10	3100.0
11	3012.5
12	2925.0
13	2821.0
14	2717.0
15	2649.5
16	2582.0
17	2357.0
18	2132.0
19	2040.5
20	1949.0
21	1896.5
22	1844.0
23	1849.5
24	1855.0
25	1838.0
26	2144.5
27	2468.0
28	2848.0
29	3228.0
30	3462.5
31	3697.0
32	4473.5
33	5250.0
34	5715.0
35	6180.0
36	6738.0
37	7296.0
38	7940.0
39	8584.0
40	9265.0
41	9946.0
42	12107.0
43	14268.0
44	15265.5
45	16263.0
46	20498.0
47	24733.0
48	25754.0
49	26775.0
50	26834.5
51	26894.0
52	24479.5
53	22065.0
54	20269.0
55	18473.0
56	16541.5
57	14610.0
58	13246.0
59	11882.0
60	11029.5
61	10177.0
62	9027.5
63	7878.0
64	6639.5
65	5401.0
66	4358.0
67	3315.0
68	2715.5
69	2116.0
70	1730.5
71	1345.0
72	1038.5
73	732.0
74	646.0
75	456.5
76	353.0
77	299.0
78	245.0
79	162.0
80	79.0
81	52.5
82	26.0
83	18.0
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	295600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.44572717785891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3304297364205	26.725808360087346
2	9.937356499070539	7.243483676684962
3	3.3946759119342382	3.71164296430814
4	1.97941780669413	2.8856528541508055
5	1.445655388106254	2.6343980934061135
6	1.1475839123937812	2.5094718110882224
7	0.9643812673858951	2.4603303596608903
8	0.8230460580493761	2.3997209669183834
9	0.7742779394525746	2.539721028701097
>10	6.074792740430594	38.05407248326328
>50	0.09255499864944569	2.3253553383619012
>100	0.028861236137999194	1.7747564608648858
>500	0.001990430078482703	0.41639493502409336
>1k	0.003980860156965406	1.657600046219605
>5k	9.952150392413515E-4	2.6615906212602765
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7338	2.482408660351827	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1390	0.4702300405953992	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1121	0.37922868741542626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	1033	0.34945872801082545	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	1026	0.3470906630581867	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	644	0.21786197564276047	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	504	0.17050067658998647	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	446	0.15087956698240867	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	329	0.11129905277401893	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17828146143437076	0.0
2	0.0	0.0	0.0	1.0642760487144791	0.0
3	0.0	0.0	0.0	1.3433694181326117	0.0
4	0.0	0.0	0.0	1.7510148849797023	0.0
5	0.0	0.0	0.0	2.95297699594046	0.0
6	0.0	0.0	0.0	3.644790257104195	0.0
7	0.0	0.0	0.0	4.345060893098782	0.0
8	0.0	0.0	0.0	5.558186738836265	0.0
9	0.0	0.0	0.0	6.074424898511502	0.0
10	0.0	0.0	0.0	7.54702300405954	0.0
11	0.0	0.0	0.0	8.83085250338295	0.0
12	0.0	0.0	0.0	10.270297699594046	0.0
13	0.0	0.0	0.0	10.775710419485792	0.0
14	0.0	0.0	0.0	10.969553450608931	0.0
15	0.0	0.0	0.0	11.426251691474967	0.0
16	0.0	0.0	0.0	12.135994587280107	0.0
17	0.0	0.0	0.0	12.958728010825439	0.0
18	3.3829499323410016E-4	0.0	0.0	13.91914749661705	0.0
19	3.3829499323410016E-4	0.0	0.0	14.568335588633289	0.0
20	3.3829499323410016E-4	0.0	0.0	15.096752368064953	0.0
21	3.3829499323410016E-4	0.0	0.0	15.696887686062245	0.0
22	3.3829499323410016E-4	0.0	0.0	16.239512855209743	0.0
23	3.3829499323410016E-4	0.0	0.0	16.805480378890394	0.0
24	3.3829499323410016E-4	0.0	0.0	17.259472259810554	0.0
25	3.3829499323410016E-4	0.0	0.0	17.679972936400542	0.0
26	3.3829499323410016E-4	0.0	0.0	18.0882949932341	0.0
27	3.3829499323410016E-4	0.0	0.0	18.528755074424897	0.0
28	3.3829499323410016E-4	0.0	0.0	18.939445196211096	0.0
29	3.3829499323410016E-4	0.0	0.0	19.365020297699594	0.0
30	3.3829499323410016E-4	0.0	0.0	19.847090663058186	0.0
31	3.3829499323410016E-4	0.0	0.0	20.253044654939107	0.0
32	3.3829499323410016E-4	0.0	0.0	20.660690121786196	0.0
33	3.3829499323410016E-4	0.0	0.0	21.093031123139376	0.0
34	3.3829499323410016E-4	0.0	0.0	21.50913396481732	0.0
35	3.3829499323410016E-4	0.0	0.0	21.970568335588634	0.0
36	3.3829499323410016E-4	0.0	0.0	22.353518267929633	0.0
37	3.3829499323410016E-4	0.0	0.0	22.78044654939107	0.0
38	3.3829499323410016E-4	0.0	0.0	23.181326116373477	0.0
39	3.3829499323410016E-4	0.0	0.0	23.670162381596754	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.024775E-4	45.000004	1
CGGTTCG	20	7.024775E-4	45.000004	30
TTGTCCG	20	7.024775E-4	45.000004	25
ATTCGAC	20	7.024775E-4	45.000004	42
CAATTAG	20	7.024775E-4	45.000004	36
AGTATCG	20	7.024775E-4	45.000004	28
GACCATA	20	7.024775E-4	45.000004	9
TATTGCG	20	7.024775E-4	45.000004	1
TCAGATC	20	7.024775E-4	45.000004	17
GCGATAT	20	7.024775E-4	45.000004	9
GCGATAC	20	7.024775E-4	45.000004	9
CGGTCTA	20	7.024775E-4	45.000004	31
ACCCGGG	20	7.024775E-4	45.000004	3
GTGCGGT	20	7.024775E-4	45.000004	12
TCGAATG	20	7.024775E-4	45.000004	44
CGAAAGG	50	2.1827873E-11	45.0	2
GCGCGAC	25	3.8837527E-5	45.0	9
GGCCGAT	25	3.8837527E-5	45.0	8
TTCACGG	25	3.8837527E-5	45.0	2
GCGTATG	25	3.8837527E-5	45.0	1
>>END_MODULE