FastQCFastQC Report
Sat 18 Jun 2016
SRR3554420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759007
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76951.0138246419334735No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC12250.16139508594782392No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC11320.14914223452484626No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG8220.10829939644825409No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC7700.10144833973863218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG351.2111923E-745.0000041
TTAACGG351.2111923E-745.0000042
AGCGTGC207.03177E-445.044
TTGCGAA207.03177E-445.037
ACGTATT207.03177E-445.029
CGATCGA253.8895487E-545.041
CCGCATG253.8895487E-545.024
AAGTCGT207.03177E-445.011
CGTTTTT42550.042.144541
TTTACGG700.041.7857172
ACGGGCT1300.041.538465
TCATGCG403.4561526E-739.3751
TCGTCGG403.4561526E-739.3752
GTAACGG750.039.02
TCGGGTC356.2454837E-638.571435
ACGATCG356.2454837E-638.5714340
GCTTACG356.2454837E-638.571431
TTACGGG2400.038.4375043
AACGACG2600.036.34615313
CGCGCAA2550.036.1764718
Produced by FastQC (version 0.11.5)