SRR3554418_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350760
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1918	0.5468126354202303	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1663	0.47411335386018927	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1224	0.34895655148819704	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	869	0.24774774774774774	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	724	0.20640894058615578	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	499	0.1422625156802372	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	450	0.12829284981183714	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	372	0.10605542251111871	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGGT	30	2.1611722E-6	45.000004	19
TAACGGG	60	0.0	45.000004	3
TACGCCG	30	2.1611722E-6	45.000004	13
ACAACGA	35	1.2087003E-7	45.0	13
CACGACG	35	1.2087003E-7	45.0	26
TTATCGG	20	7.026578E-4	45.0	2
GACCATA	20	7.026578E-4	45.0	28
GCATTAT	20	7.026578E-4	45.0	18
CACTAGA	20	7.026578E-4	45.0	11
TTGCGAG	20	7.026578E-4	45.0	1
CGATGAT	20	7.026578E-4	45.0	10
GCTACGA	20	7.026578E-4	45.0	10
TATCGGG	40	6.7921064E-9	45.0	3
AATGTAC	20	7.026578E-4	45.0	20
ACTGGTC	20	7.026578E-4	45.0	39
CGCCTCA	25	3.8852464E-5	44.999996	36
ACGGACT	25	3.8852464E-5	44.999996	45
TGTAACG	25	3.8852464E-5	44.999996	1
CGTCACG	25	3.8852464E-5	44.999996	21
CGTAAGG	55	6.002665E-11	40.909092	2