FastQCFastQC Report
Sat 18 Jun 2016
SRR3554417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574359
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC113961.984124911422995No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC67921.1825356614939437No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG58901.025491025647722No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT35660.6208660437113374No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.600669616041535No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC32740.5700267602666625TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT18090.3149598073678657No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT16120.28066070175621866No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT15680.2729999878125005No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC15450.26899552370555696TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC14150.24636159614457162No Hit
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG11050.1923883842683757No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT10440.18176784902822102TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA9820.17097320665298182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGA253.8883594E-545.00000425
TAGTACG253.8883594E-545.0000041
GCGAACG207.0303376E-445.01
AATTGCG207.0303376E-445.01
TGTAGCG351.210501E-745.01
GTACGAG302.1635988E-644.9999961
CGTTTTT25500.042.4411771
CGGTCGA556.002665E-1140.9090941
CCGATGA9200.040.8423918
ACCGCGG501.0786607E-940.5000042
GCTACGA451.9254003E-840.010
TAGTGCG451.9254003E-840.01
CGTAAGG850.039.7058832
CGATGAA9700.039.6649519
TCGCAGG403.454188E-739.3752
GTTTACG403.454188E-739.3751
TACGAGG800.039.3752
CGTTTCT1200.039.3749961
TGAATGA10700.038.90186722
TCGATAG356.2428335E-638.5714261
Produced by FastQC (version 0.11.5)