##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574359
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.034786605589886	33.0	31.0	34.0	30.0	34.0
2	32.09132093342317	33.0	31.0	34.0	30.0	34.0
3	32.16644642114078	34.0	31.0	34.0	30.0	34.0
4	35.762773805233316	37.0	35.0	37.0	35.0	37.0
5	35.82351978466429	37.0	35.0	37.0	35.0	37.0
6	35.826977552367076	37.0	35.0	37.0	35.0	37.0
7	36.034375712751086	37.0	35.0	37.0	35.0	37.0
8	35.82607393633599	37.0	35.0	37.0	35.0	37.0
9	37.66042666694524	39.0	38.0	39.0	35.0	39.0
10	37.42237172221555	39.0	37.0	39.0	34.0	39.0
11	37.40999096383969	39.0	37.0	39.0	34.0	39.0
12	37.408786142464905	39.0	37.0	39.0	35.0	39.0
13	37.36856565318903	39.0	37.0	39.0	34.0	39.0
14	38.627614436267216	40.0	38.0	41.0	34.0	41.0
15	38.65360166724992	40.0	38.0	41.0	34.0	41.0
16	38.773801751169565	40.0	38.0	41.0	35.0	41.0
17	38.685781889027595	40.0	38.0	41.0	34.0	41.0
18	38.58904448263194	40.0	38.0	41.0	35.0	41.0
19	38.53134363699359	40.0	38.0	41.0	34.0	41.0
20	38.28561579082072	40.0	37.0	41.0	34.0	41.0
21	38.37183538518592	40.0	37.0	41.0	34.0	41.0
22	38.3855863667149	40.0	37.0	41.0	34.0	41.0
23	38.2866012372053	40.0	37.0	41.0	34.0	41.0
24	38.26554297921683	40.0	37.0	41.0	34.0	41.0
25	38.2344700788183	40.0	37.0	41.0	34.0	41.0
26	38.159541332163336	40.0	37.0	41.0	34.0	41.0
27	38.08969999599554	40.0	37.0	41.0	34.0	41.0
28	37.97717107244772	40.0	37.0	41.0	33.0	41.0
29	37.98920013441071	40.0	37.0	41.0	34.0	41.0
30	37.8401174178519	40.0	37.0	41.0	33.0	41.0
31	37.687286174674725	40.0	36.0	41.0	33.0	41.0
32	37.468402166589186	40.0	36.0	41.0	33.0	41.0
33	37.3130672628095	40.0	36.0	41.0	32.0	41.0
34	37.05527205110393	40.0	36.0	41.0	31.0	41.0
35	36.951349243243335	40.0	36.0	41.0	31.0	41.0
36	36.790413312927974	40.0	36.0	41.0	30.0	41.0
37	36.717901173307986	40.0	36.0	41.0	30.0	41.0
38	36.588529125512096	40.0	35.0	41.0	30.0	41.0
39	36.519293682174386	40.0	35.0	41.0	30.0	41.0
40	36.40280208023205	40.0	35.0	41.0	30.0	41.0
41	36.35889400183509	39.0	35.0	41.0	29.0	41.0
42	36.30614824526124	39.0	35.0	41.0	29.0	41.0
43	36.23521699842781	39.0	35.0	41.0	29.0	41.0
44	36.258155613475196	39.0	35.0	41.0	29.0	41.0
45	36.23238775748269	39.0	35.0	41.0	29.0	41.0
46	36.13075794059117	39.0	35.0	41.0	29.0	41.0
47	35.9727017422901	39.0	35.0	41.0	28.0	41.0
48	35.948589645152246	39.0	35.0	41.0	28.0	41.0
49	35.869583309393605	39.0	35.0	41.0	28.0	41.0
50	35.74709893986165	39.0	35.0	41.0	28.0	41.0
51	34.902693263272624	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	3.0
11	3.0
12	7.0
13	10.0
14	12.0
15	16.0
16	34.0
17	63.0
18	148.0
19	371.0
20	848.0
21	1621.0
22	2247.0
23	2901.0
24	3884.0
25	5109.0
26	6470.0
27	7338.0
28	7059.0
29	7178.0
30	7849.0
31	9678.0
32	11907.0
33	15902.0
34	25527.0
35	35503.0
36	37867.0
37	55990.0
38	104738.0
39	223750.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14319441325025	16.464963550671268	19.87520000557143	18.516642030507054
2	29.760654921399333	30.96234236775257	22.812387374446992	16.4646153364011
3	28.346208555972837	29.946079020264328	26.803097017718887	14.904615406043956
4	25.86779348804493	22.823704338227486	33.90858330765253	17.39991886607505
5	28.70260586149081	26.308980968349065	25.501646182962222	19.4867669871979
6	23.237905212593517	35.58123055440935	26.25518186360795	14.925682369389179
7	73.33740744029431	4.822419427570561	16.89953495984219	4.940638172292939
8	72.8791574607519	9.407182615750775	11.759892332147663	5.953767591349661
9	66.49029613882607	7.199852357149449	13.560160108921423	12.749691395103063
10	34.60274845523445	29.457882613487385	20.538548190243382	15.400820741034787
11	27.337431815293222	23.367092706826217	30.471360246814278	18.824115231066287
12	22.32436507480513	20.828958891564337	33.77434670650238	23.072329327128156
13	23.245217712267067	22.23765972153305	36.586525152387274	17.930597413812617
14	20.273731237779856	28.454851408265565	30.300909361566546	20.970507992388036
15	18.147883118398074	24.523512298057486	36.26790909518263	21.06069548836181
16	20.469427657614837	25.763503314129316	30.145605797071166	23.621463231184677
17	20.776378536768817	26.801704160638206	31.184328964985315	21.23758833760766
18	21.271713336084225	24.468146229100615	30.79902987504331	23.46111055977185
19	20.430949980761163	26.47281578246358	28.803762106974908	24.292472129800352
20	23.85163286376639	25.998896160763564	31.361221814231172	18.788249161238877
21	22.109168655840687	29.416793329607437	29.691360281635703	18.782677732916174
22	20.835052641292293	23.23459717702691	31.630391445071808	24.299958736608986
23	21.380356188376958	27.337954136698478	30.171721867333844	21.109967807590724
24	22.240967757099654	25.65573099751201	29.595601357339223	22.507699888049114
25	20.2676374880519	30.630320061146428	27.401155026734152	21.700887424067524
26	21.329342797797196	25.034690846665587	30.75376201992134	22.882204335615878
27	25.586436357748376	25.31448101274638	27.474454130604727	21.624628498900513
28	20.46768658626399	27.882387148107718	29.62990046295087	22.02002580267742
29	24.66001229196374	23.559481091094593	29.379360295564272	22.401146321377396
30	24.893489960112056	27.233663962782863	28.1431996364643	19.72964644064078
31	24.688565862117596	26.110324727217648	26.053217586909927	23.14789182375483
32	25.43235154319859	26.852195229812715	28.791748714654076	18.92370451233462
33	24.792681928898126	26.84523094440933	26.019092588433367	22.342994538259173
34	25.970690804879876	25.777431884936075	27.561507698146976	20.69036961203707
35	25.43496315022486	24.006065892586346	29.486784397911407	21.072186559277387
36	26.091521156628524	28.777820143847315	25.97643634033766	19.1542223591865
37	24.635114971646654	24.78188728652289	30.822534338279716	19.76046340355074
38	21.894668665416578	28.312605878901525	27.487512165736067	22.305213289945836
39	26.131043476292703	25.012579240509858	28.847114783610948	20.009262499586494
40	23.670909657548673	25.071427452168415	30.730431663820013	20.52723122646289
41	24.438373909001164	25.72485153014056	27.263610390017394	22.573164170840883
42	23.981690893674514	24.011289106638877	30.963387010563082	21.04363298912353
43	26.582155063296646	23.997882857237375	27.392971991385178	22.0269900880808
44	24.093467674398763	24.005021249775837	28.744739788181263	23.15677128764414
45	22.404802571214173	22.944012368570878	28.43848533756762	26.212699722647333
46	26.27207025571115	24.828199784455368	27.30905235227445	21.590677607559037
47	21.685740103315172	24.326074806871663	32.410043195980215	21.57814189383295
48	22.828405230874765	24.361244448158732	28.4262978381117	24.384052482854802
49	22.70983827188222	22.10481597746357	32.86446281855076	22.32088293210344
50	22.884467728371977	22.293199897625005	31.438351275073607	23.383981098929414
51	21.692356174448385	21.75015974329644	29.15075762719832	27.406726455056855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	264.0
2	427.0
3	3381.0
4	6335.0
5	4403.0
6	2471.0
7	2410.5
8	2350.0
9	2333.5
10	2317.0
11	2311.0
12	2305.0
13	2200.0
14	2095.0
15	2016.5
16	1938.0
17	1875.0
18	1812.0
19	1759.0
20	1706.0
21	1775.5
22	1845.0
23	2010.0
24	2175.0
25	2577.5
26	3692.0
27	4404.0
28	5348.5
29	6293.0
30	7421.5
31	8550.0
32	9741.5
33	10933.0
34	12877.5
35	14822.0
36	15884.0
37	16946.0
38	18297.5
39	19649.0
40	21237.0
41	22825.0
42	25331.0
43	27837.0
44	31631.5
45	35426.0
46	56978.0
47	78530.0
48	67589.5
49	56649.0
50	56118.0
51	55587.0
52	48232.5
53	40878.0
54	37123.0
55	33368.0
56	29855.0
57	26342.0
58	23732.0
59	21122.0
60	19225.0
61	17328.0
62	15517.5
63	13707.0
64	11886.5
65	10066.0
66	8318.5
67	6571.0
68	5682.0
69	4793.0
70	4179.0
71	3565.0
72	3083.5
73	2602.0
74	2327.0
75	1669.5
76	1287.0
77	983.0
78	679.0
79	531.5
80	384.0
81	270.5
82	157.0
83	128.0
84	99.0
85	64.0
86	29.0
87	21.0
88	13.0
89	8.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	574359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.09987850617331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.26227353165248	29.288707842246076
2	9.627121006641769	7.9134700747441125
3	4.562197990861538	5.625173494365517
4	3.0696489307583086	5.046487924430852
5	2.3430583297216963	4.814970634221955
6	1.7519642943133802	4.320331178606016
7	1.4579756318634802	4.194583493418521
8	1.2088542337552353	3.9747009711210723
9	1.000352069890689	3.7002913682315675
>10	3.664159039362412	20.87465319585604
>50	0.031096184622506767	0.8750261573685407
>100	0.01448315445841978	1.2299037110606021
>500	0.0012779253933899806	0.3568033826535953
>1k	0.0042597513112999355	3.569434428626841
>5k	8.519502622599871E-4	2.2203044645794385
>10k+	4.2597513112999357E-4	1.99515767846927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	11396	1.984124911422995	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	6792	1.1825356614939437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	5890	1.025491025647722	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	3566	0.6208660437113374	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3450	0.600669616041535	No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3274	0.5700267602666625	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT	1809	0.3149598073678657	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	1612	0.28066070175621866	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	1568	0.2729999878125005	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	1545	0.26899552370555696	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC	1415	0.24636159614457162	No Hit
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1105	0.1923883842683757	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	1044	0.18176784902822102	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA	982	0.17097320665298182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.482142701690058E-4	0.0	0.0	0.19012499151227716	0.0
2	3.482142701690058E-4	0.0	0.0	1.166691912201254	0.0
3	3.482142701690058E-4	0.0	0.0	1.6313838557417921	0.0
4	3.482142701690058E-4	0.0	0.0	2.622749882912952	0.0
5	3.482142701690058E-4	0.0	0.0	5.529294396013643	0.0
6	5.223214052535087E-4	0.0	0.0	6.914838977016117	0.0
7	5.223214052535087E-4	0.0	0.0	8.089017496026004	0.0
8	5.223214052535087E-4	0.0	0.0	9.794048669908541	0.0
9	6.964285403380116E-4	0.0	0.0	10.574396849357283	0.0
10	6.964285403380116E-4	0.0	0.0	12.780160143742851	0.0
11	6.964285403380116E-4	0.0	0.0	13.897405629580106	0.0
12	6.964285403380116E-4	0.0	0.0	16.17350820653981	0.0
13	6.964285403380116E-4	0.0	0.0	16.79907514289843	0.0
14	6.964285403380116E-4	0.0	0.0	17.14746352020252	0.0
15	6.964285403380116E-4	0.0	0.0	17.647150997895046	0.0
16	6.964285403380116E-4	0.0	0.0	18.232150971778974	0.0
17	6.964285403380116E-4	0.0	0.0	18.89271344228958	0.0
18	6.964285403380116E-4	0.0	0.0	19.6081196603518	0.0
19	6.964285403380116E-4	0.0	0.0	20.402396410607302	0.0
20	6.964285403380116E-4	0.0	0.0	20.914619602025912	0.0
21	6.964285403380116E-4	0.0	0.0	21.408909758530815	0.0
22	6.964285403380116E-4	0.0	0.0	21.983463304309673	0.0
23	8.705356754225144E-4	0.0	0.0	22.540954350850253	0.0
24	8.705356754225144E-4	0.0	0.0	22.960726653538988	0.0
25	8.705356754225144E-4	0.0	0.0	23.36831145677181	0.0
26	8.705356754225144E-4	0.0	0.0	23.748213225526197	0.0
27	8.705356754225144E-4	0.0	0.0	24.184177491777792	0.0
28	8.705356754225144E-4	0.0	0.0	24.594199794901794	0.0
29	8.705356754225144E-4	0.0	0.0	25.016061383211547	0.0
30	8.705356754225144E-4	0.0	0.0	25.505650647069167	0.0
31	0.0010446428105070173	0.0	0.0	25.959199733964297	0.0
32	0.0010446428105070173	0.0	0.0	26.435905069825665	0.0
33	0.0012187499455915203	0.0	0.0	26.84279344451815	0.0
34	0.0012187499455915203	0.0	0.0	27.25960592591045	0.0
35	0.0012187499455915203	0.0	0.0	27.68111929995003	0.0
36	0.0012187499455915203	0.0	0.0	28.099324638423006	0.0
37	0.0012187499455915203	0.0	0.0	28.52083801246259	0.0
38	0.0012187499455915203	0.0	0.0	28.95471299309317	0.0
39	0.0012187499455915203	0.0	0.0	29.38928440226409	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATGA	25	3.8883594E-5	45.000004	25
TAGTACG	25	3.8883594E-5	45.000004	1
GCGAACG	20	7.0303376E-4	45.0	1
AATTGCG	20	7.0303376E-4	45.0	1
TGTAGCG	35	1.210501E-7	45.0	1
GTACGAG	30	2.1635988E-6	44.999996	1
CGTTTTT	2550	0.0	42.441177	1
CGGTCGA	55	6.002665E-11	40.90909	41
CCGATGA	920	0.0	40.84239	18
ACCGCGG	50	1.0786607E-9	40.500004	2
GCTACGA	45	1.9254003E-8	40.0	10
TAGTGCG	45	1.9254003E-8	40.0	1
CGTAAGG	85	0.0	39.705883	2
CGATGAA	970	0.0	39.66495	19
TCGCAGG	40	3.454188E-7	39.375	2
GTTTACG	40	3.454188E-7	39.375	1
TACGAGG	80	0.0	39.375	2
CGTTTCT	120	0.0	39.374996	1
TGAATGA	1070	0.0	38.901867	22
TCGATAG	35	6.2428335E-6	38.571426	1
>>END_MODULE