SRR3554416_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235897
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	9394	3.9822464889337295	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	7753	3.2866038991593785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	7664	3.24887556857442	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3587	1.5205788967218743	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	2298	0.9741539739801692	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	1987	0.8423167738462125	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	1421	0.6023815478789472	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	1344	0.569740183215556	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1327	0.5625336481600021	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1308	0.5544792854508536	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.3628702357384791	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	832	0.3526963038953442	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTCTTGC	512	0.2170438793202118	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	467	0.1979677571143338	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATG	421	0.17846772108165854	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGT	354	0.15006549468624017	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTAT	328	0.1390437351895107	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	323	0.1369241660555242	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	311	0.13183720013395678	TruSeq Adapter, Index 27 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	308	0.13056545865356492	TruSeq Adapter, Index 27 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	279	0.11827195767644352	No Hit
TGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCTT	265	0.11233716410128149	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC	261	0.11064150879409233	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTCG	20	7.0218736E-4	45.000004	40
CGAACCC	20	7.0218736E-4	45.000004	34
CGAGTCA	20	7.0218736E-4	45.000004	16
ACTTGCA	20	7.0218736E-4	45.000004	16
ACCGCTC	30	2.1581418E-6	45.000004	39
AGGGTGA	60	0.0	45.000004	6
CCGTAGC	20	7.0218736E-4	45.000004	15
CCAAGCG	35	1.2064447E-7	45.000004	20
GACATTC	20	7.0218736E-4	45.000004	9
ATGGTCC	35	1.2064447E-7	45.000004	11
ATTGGGC	20	7.0218736E-4	45.000004	4
GTCCAAA	20	7.0218736E-4	45.000004	13
AAGTGAT	20	7.0218736E-4	45.000004	22
CACCCGT	35	1.2064447E-7	45.000004	38
GTAGTAG	20	7.0218736E-4	45.000004	1
CACTTGT	20	7.0218736E-4	45.000004	18
TGCCCCG	25	3.88135E-5	45.0	30
ATAGGGT	25	3.88135E-5	45.0	4
TTAGCGG	25	3.88135E-5	45.0	2
ACTAGTG	25	3.88135E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content