##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554416_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235897 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80808573233233 33.0 31.0 34.0 30.0 34.0 2 31.87381781031552 33.0 31.0 34.0 30.0 34.0 3 31.982941707609676 33.0 31.0 34.0 30.0 34.0 4 35.6529502282776 37.0 35.0 37.0 33.0 37.0 5 35.68833855453863 37.0 35.0 37.0 35.0 37.0 6 35.69236149675494 37.0 35.0 37.0 35.0 37.0 7 35.871405740641045 37.0 35.0 37.0 35.0 37.0 8 35.512401599002956 37.0 35.0 37.0 33.0 37.0 9 37.386821366952525 39.0 37.0 39.0 34.0 39.0 10 37.25859591262288 39.0 37.0 39.0 34.0 39.0 11 37.238705875869556 39.0 37.0 39.0 34.0 39.0 12 37.09688974425279 39.0 37.0 39.0 34.0 39.0 13 36.89497534941097 39.0 37.0 39.0 33.0 39.0 14 37.97873224330958 40.0 37.0 41.0 33.0 41.0 15 37.941940762281845 40.0 37.0 41.0 33.0 41.0 16 38.271194631555296 40.0 37.0 41.0 34.0 41.0 17 38.103362908387986 40.0 37.0 41.0 33.0 41.0 18 38.06758034226802 40.0 37.0 41.0 34.0 41.0 19 38.06319707329894 40.0 37.0 41.0 34.0 41.0 20 37.679737342992915 40.0 36.0 41.0 32.0 41.0 21 37.89246577955633 40.0 36.0 41.0 33.0 41.0 22 37.89643785211342 40.0 36.0 41.0 33.0 41.0 23 37.697626506483765 40.0 36.0 41.0 33.0 41.0 24 37.67997049559766 40.0 35.0 41.0 33.0 41.0 25 37.751700954230024 40.0 36.0 41.0 33.0 41.0 26 37.64051683573763 40.0 35.0 41.0 33.0 41.0 27 37.46797966909287 40.0 35.0 41.0 33.0 41.0 28 37.40223487369487 40.0 35.0 41.0 32.0 41.0 29 37.468234017388944 40.0 36.0 41.0 33.0 41.0 30 37.35069543063286 40.0 36.0 41.0 32.0 41.0 31 37.36559176250652 40.0 35.0 41.0 32.0 41.0 32 37.061666744384205 40.0 35.0 41.0 31.0 41.0 33 36.92156746376597 40.0 35.0 41.0 31.0 41.0 34 36.8408754668351 40.0 35.0 41.0 31.0 41.0 35 36.73426537853385 40.0 35.0 41.0 31.0 41.0 36 36.60843503732561 40.0 35.0 41.0 30.0 41.0 37 36.60624764197934 40.0 35.0 41.0 30.0 41.0 38 36.51019300796534 39.0 35.0 41.0 30.0 41.0 39 36.46151922237247 39.0 35.0 41.0 30.0 41.0 40 36.31303916539846 39.0 35.0 41.0 30.0 41.0 41 36.30766393807467 39.0 35.0 41.0 30.0 41.0 42 36.323840489705255 39.0 35.0 41.0 30.0 41.0 43 36.17750119755656 39.0 35.0 41.0 30.0 41.0 44 36.342615633094105 39.0 35.0 41.0 30.0 41.0 45 36.29410293475542 39.0 35.0 41.0 30.0 41.0 46 36.17257107975091 39.0 35.0 41.0 30.0 41.0 47 35.90727308952636 39.0 35.0 41.0 30.0 41.0 48 36.05688499641793 39.0 35.0 41.0 30.0 41.0 49 36.04632954213068 39.0 35.0 41.0 30.0 41.0 50 35.82887446639847 39.0 35.0 41.0 29.0 41.0 51 35.24141892436106 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 4.0 12 1.0 13 2.0 14 3.0 15 4.0 16 8.0 17 21.0 18 45.0 19 136.0 20 373.0 21 743.0 22 1190.0 23 1589.0 24 1758.0 25 2125.0 26 2481.0 27 2559.0 28 2537.0 29 2766.0 30 3330.0 31 4412.0 32 5978.0 33 8130.0 34 13448.0 35 20601.0 36 16640.0 37 22879.0 38 39519.0 39 82526.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.070619804406164 14.471994133032636 17.531804134855467 14.925581927705736 2 25.479340559651032 38.64398445084083 19.14945929791392 16.727215691594214 3 25.589134240791534 37.680852236357396 22.868031386579737 13.861982136271338 4 24.73749136275578 19.649253699707923 39.24594208489298 16.367312852643316 5 35.195869383671685 21.365680784410145 22.6238570223445 20.81459280957367 6 21.045201931351396 39.61220363124584 24.720534809683887 14.622059627718878 7 61.978744960724384 4.998367931766831 26.27841812316392 6.744468984344862 8 57.89433523953251 18.289762057169018 14.591537832189472 9.224364871109 9 53.34955510243877 7.09970877120099 15.131604047529217 24.419132078831016 10 40.127682844631344 18.53351250757746 23.648880655540342 17.689923992250854 11 33.93048661068178 19.601775351106625 26.98889769687618 19.478840341335413 12 25.173698690530188 16.51186746758119 31.473905984391493 26.840527857497126 13 25.31994896077525 21.181278269753324 37.99497238201418 15.503800387457236 14 20.17533075876336 31.661699809662693 25.92741747457577 22.235551956998183 15 14.614429178836527 21.511931054655207 42.62156788767979 21.25207187882847 16 16.478802189091002 22.843868298452293 28.53448750937909 32.14284200307762 17 16.939596518819656 25.208883538154364 32.313679275276925 25.537840667749062 18 17.735282771718165 22.58019389818438 32.29757054985862 27.386952780238833 19 18.07526166080959 24.70442608426559 28.26954136763079 28.95077088729403 20 21.51023539934802 24.281783998948693 35.08819527166517 19.119785330038113 21 19.993047813240526 32.118255001123366 28.815118462718896 19.073578722917205 22 17.32493418737839 22.25293242389687 31.907145915378322 28.51498747334642 23 19.31097046592369 29.61843516449976 29.200456131277637 21.87013823829892 24 22.11219303340017 22.640813575416388 26.582364336977584 28.66462905420586 25 15.635637587591194 34.721509811485525 26.1101243339254 23.532728266997886 26 15.856072777525783 21.323713315557214 33.26239841964925 29.557815487267746 27 23.737478645340975 26.0791786245692 26.71547327859193 23.4678694514979 28 14.506331153003218 24.876111184118493 35.72067470124673 24.896882961631558 29 19.802286591181744 18.909524071946652 29.504826258918087 31.783363077953513 30 17.358423379695374 26.15845051018029 35.54559829077945 20.937527819344883 31 21.129984696710853 19.70266684188438 29.714663603182746 29.45268485822202 32 24.964709173919125 24.22455563233106 25.180905225585743 25.62982996816407 33 19.692492910041246 18.000228913466472 30.995731187764154 31.311546988728132 34 20.97525614992984 18.01591372505797 32.6133863508226 28.395443774189584 35 22.717965891893495 17.608532537505774 30.401827916421148 29.27167365417958 36 19.248655133384485 19.762014777636004 31.983874318028633 29.00545577095088 37 17.721293615433854 18.073989919329197 38.88264793532771 25.32206852990924 38 19.049839548616557 20.46783129925349 32.29163575628346 28.190693395846495 39 20.834516759433143 19.68231897819811 31.188611978956914 28.29455228341183 40 23.423358499684184 16.89678122231313 34.7549990038025 24.92486127420018 41 18.418207946688597 20.872669003844898 29.2682823435652 31.4408407059013 42 24.198272975069628 17.4796627341594 32.767267069950016 25.55479722082095 43 22.550519930308567 18.499175487606877 26.592538268820714 32.357766313263845 44 20.858679847560587 18.149446580499117 34.33913953971437 26.65273403222593 45 18.8400022043519 16.942987829434035 31.3946340987804 32.82237586743367 46 28.134312856882453 21.684463982161706 27.003734680814084 23.177488480141754 47 16.45718258392434 18.814143460917265 41.37102209862779 23.3576518565306 48 21.15711518162588 20.734473096308985 28.912618642882276 29.195793079182863 49 20.4347660207633 14.80434257324171 39.18998545975574 25.570905946239247 50 23.21606463838031 16.638617701793578 31.933428572639755 28.21188908718636 51 19.907417220227472 16.197323408097603 27.92066028817662 35.974599083498305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40.0 1 140.0 2 240.0 3 909.5 4 1579.0 5 1069.0 6 559.0 7 551.5 8 544.0 9 581.5 10 619.0 11 598.5 12 578.0 13 564.5 14 551.0 15 541.5 16 532.0 17 532.5 18 533.0 19 542.0 20 551.0 21 519.5 22 488.0 23 550.5 24 613.0 25 689.5 26 784.0 27 802.0 28 1292.0 29 1782.0 30 1887.5 31 1993.0 32 2262.5 33 2532.0 34 2857.0 35 3182.0 36 3759.5 37 4337.0 38 4651.5 39 4966.0 40 5980.5 41 6995.0 42 8233.5 43 9472.0 44 11566.0 45 13660.0 46 32733.0 47 51806.0 48 39961.5 49 28117.0 50 28076.5 51 28036.0 52 22649.0 53 17262.0 54 14746.5 55 12231.0 56 11158.5 57 10086.0 58 9071.5 59 8057.0 60 6820.5 61 5584.0 62 4981.5 63 4379.0 64 3806.0 65 3233.0 66 2804.5 67 2376.0 68 2232.0 69 2088.0 70 1792.5 71 1497.0 72 1324.0 73 1151.0 74 985.0 75 701.0 76 583.0 77 437.0 78 291.0 79 269.0 80 247.0 81 153.0 82 59.0 83 51.0 84 43.0 85 36.0 86 29.0 87 17.5 88 6.0 89 4.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 235897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.94024934611292 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56535190420149 26.053319881134563 2 10.350266309161277 7.232817712815338 3 3.9224488310301737 4.111540206106903 4 2.0091479320092693 2.80800518870524 5 1.2375186537738254 2.161960516666172 6 0.9196462152554506 1.9279600842740687 7 0.7340184171893768 1.7952750564865176 8 0.6223990876333062 1.7397423451760725 9 0.5799352122587141 1.8236772828819359 >10 4.936728825691858 29.00376011564369 >50 0.06794220059934727 1.6100247141761022 >100 0.03882411462819844 2.6553962110582163 >500 0.0036397607463936037 0.9326104189540351 >1k 0.00849277507491841 5.626184309253615 >5k 0.0036397607463936037 10.517725956667528 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC 9394 3.9822464889337295 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC 7753 3.2866038991593785 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG 7664 3.24887556857442 No Hit GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC 3587 1.5205788967218743 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT 2298 0.9741539739801692 No Hit GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT 1987 0.8423167738462125 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA 1421 0.6023815478789472 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC 1344 0.569740183215556 No Hit CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC 1327 0.5625336481600021 Illumina Single End Adapter 1 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT 1308 0.5544792854508536 TruSeq Adapter, Index 27 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.3628702357384791 No Hit CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT 832 0.3526963038953442 TruSeq Adapter, Index 27 (95% over 24bp) GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTCTTGC 512 0.2170438793202118 No Hit TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC 467 0.1979677571143338 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATG 421 0.17846772108165854 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGT 354 0.15006549468624017 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTAT 328 0.1390437351895107 No Hit GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT 323 0.1369241660555242 No Hit CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG 311 0.13183720013395678 TruSeq Adapter, Index 27 (95% over 22bp) ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC 308 0.13056545865356492 TruSeq Adapter, Index 27 (95% over 23bp) GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 279 0.11827195767644352 No Hit TGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCTT 265 0.11233716410128149 Illumina Single End Adapter 1 (95% over 23bp) GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC 261 0.11064150879409233 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.2391382679728867E-4 0.0 0.0 0.3632941495652764 0.0 2 4.2391382679728867E-4 0.0 0.0 2.859298761747712 0.0 3 4.2391382679728867E-4 0.0 0.0 3.544767419678928 0.0 4 4.2391382679728867E-4 0.0 0.0 4.855932885962941 0.0 5 4.2391382679728867E-4 0.0 0.0 10.017507641046729 0.0 6 4.2391382679728867E-4 0.0 0.0 11.36173838582093 0.0 7 4.2391382679728867E-4 0.0 0.0 13.405426944810658 0.0 8 4.2391382679728867E-4 0.0 0.0 15.945094681153215 0.0 9 4.2391382679728867E-4 0.0 0.0 16.747563555280482 0.0 10 4.2391382679728867E-4 0.0 0.0 21.63995303034799 0.0 11 4.2391382679728867E-4 0.0 0.0 23.432684603873724 0.0 12 4.2391382679728867E-4 0.0 0.0 29.17120607722862 0.0 13 4.2391382679728867E-4 0.0 0.0 30.038533766855874 0.0 14 4.2391382679728867E-4 0.0 0.0 30.455241058597608 0.0 15 4.2391382679728867E-4 0.0 0.0 31.574797475169248 0.0 16 4.2391382679728867E-4 0.0 0.0 32.763027931682046 0.0 17 4.2391382679728867E-4 0.0 0.0 33.95507361263602 0.0 18 4.2391382679728867E-4 0.0 0.0 35.28107606285794 0.0 19 4.2391382679728867E-4 0.0 0.0 36.96698135203076 0.0 20 4.2391382679728867E-4 0.0 0.0 37.85465690534428 0.0 21 8.478276535945773E-4 0.0 0.0 38.58124520447483 0.0 22 8.478276535945773E-4 0.0 0.0 39.32945310877205 0.0 23 8.478276535945773E-4 0.0 0.0 40.01534568053006 0.0 24 8.478276535945773E-4 0.0 0.0 40.54693361933386 0.0 25 8.478276535945773E-4 0.0 0.0 41.03273886484355 0.0 26 8.478276535945773E-4 0.0 0.0 41.50921800616371 0.0 27 8.478276535945773E-4 0.0 0.0 42.092523431836774 0.0 28 8.478276535945773E-4 0.0 0.0 42.55501341687262 0.0 29 8.478276535945773E-4 0.0 0.0 43.00309033179735 0.0 30 8.478276535945773E-4 0.0 0.0 43.53086304615998 0.0 31 8.478276535945773E-4 0.0 0.0 44.02472265437882 0.0 32 8.478276535945773E-4 0.0 0.0 44.50840833075453 0.0 33 8.478276535945773E-4 0.0 0.0 44.96708309134919 0.0 34 8.478276535945773E-4 0.0 0.0 45.40625781591118 0.0 35 8.478276535945773E-4 0.0 0.0 45.872563025388196 0.0 36 8.478276535945773E-4 0.0 0.0 46.282487695901175 0.0 37 8.478276535945773E-4 0.0 0.0 46.7322602661331 0.0 38 8.478276535945773E-4 0.0 0.0 47.123532728267 0.0 39 8.478276535945773E-4 0.0 0.0 47.547870468891084 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCTCG 20 7.0218736E-4 45.000004 40 CGAACCC 20 7.0218736E-4 45.000004 34 CGAGTCA 20 7.0218736E-4 45.000004 16 ACTTGCA 20 7.0218736E-4 45.000004 16 ACCGCTC 30 2.1581418E-6 45.000004 39 AGGGTGA 60 0.0 45.000004 6 CCGTAGC 20 7.0218736E-4 45.000004 15 CCAAGCG 35 1.2064447E-7 45.000004 20 GACATTC 20 7.0218736E-4 45.000004 9 ATGGTCC 35 1.2064447E-7 45.000004 11 ATTGGGC 20 7.0218736E-4 45.000004 4 GTCCAAA 20 7.0218736E-4 45.000004 13 AAGTGAT 20 7.0218736E-4 45.000004 22 CACCCGT 35 1.2064447E-7 45.000004 38 GTAGTAG 20 7.0218736E-4 45.000004 1 CACTTGT 20 7.0218736E-4 45.000004 18 TGCCCCG 25 3.88135E-5 45.0 30 ATAGGGT 25 3.88135E-5 45.0 4 TTAGCGG 25 3.88135E-5 45.0 2 ACTAGTG 25 3.88135E-5 45.0 1 >>END_MODULE