FastQCFastQC Report
Sat 18 Jun 2016
SRR3554415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317136
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC98263.0983552797537963No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC80092.525414963927148RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG74292.3425281267342717No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC29900.9428131779425861RNA PCR Primer, Index 40 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT22100.6968619141314767No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT16380.5164976540033298RNA PCR Primer, Index 46 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT15160.4780283537662075TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTA14240.4490187175218203No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC13870.43735179859744716No Hit
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC10630.3351874274759094RNA PCR Primer, Index 46 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.27874476565259065No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT6970.21977952676454265RNA PCR Primer, Index 46 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATG4950.15608445588012715No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT3820.12045305484082539RNA PCR Primer, Index 46 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC3610.1138312900459109RNA PCR Primer, Index 40 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCGAATA3520.1109933908480904No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCGAATATCGT3510.11067806871499924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGG207.025553E-445.02
GAAAACG207.025553E-445.01
TCGTAAG207.025553E-445.042
TAGGACG207.025553E-445.01
ATTGCGG253.8843973E-545.02
TAGTGCG253.8843973E-545.01
GCATACG253.8843973E-545.01
ACGGCTA900.042.530
CGGCTAT800.042.187531
ATCGAAT1300.041.53846443
GATGAAT8800.041.42045220
TCGAATG1200.041.25000444
CGATGAA8150.041.1349719
CGTTTTT6150.040.975611
CGACGGG501.0768417E-940.53
CGTAAGG451.920671E-840.02
ACGGCTG11650.039.7854088
ATGAATG9050.039.77900721
CGAATGC1250.039.60000245
CCGATGA8200.039.51219618
Produced by FastQC (version 0.11.5)