##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317136
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87181524645578	33.0	31.0	34.0	30.0	34.0
2	31.92577316987034	33.0	31.0	34.0	30.0	34.0
3	32.03310251753191	33.0	31.0	34.0	30.0	34.0
4	35.66385714646083	37.0	35.0	37.0	33.0	37.0
5	35.71635512839917	37.0	35.0	37.0	35.0	37.0
6	35.71935068866354	37.0	35.0	37.0	35.0	37.0
7	35.888842641642704	37.0	35.0	37.0	35.0	37.0
8	35.57697959235155	37.0	35.0	37.0	33.0	37.0
9	37.44628172140659	39.0	37.0	39.0	34.0	39.0
10	37.27984839311841	39.0	37.0	39.0	34.0	39.0
11	37.24755310024721	39.0	37.0	39.0	34.0	39.0
12	37.11416868472832	39.0	37.0	39.0	34.0	39.0
13	36.92557136370516	39.0	37.0	39.0	33.0	39.0
14	38.04832627011755	40.0	37.0	41.0	33.0	41.0
15	38.02172254174865	40.0	37.0	41.0	33.0	41.0
16	38.28368586347813	40.0	37.0	41.0	34.0	41.0
17	38.103952247616164	40.0	37.0	41.0	33.0	41.0
18	38.083651808687755	40.0	37.0	41.0	33.0	41.0
19	38.072341203773775	40.0	37.0	41.0	34.0	41.0
20	37.75634743453912	40.0	36.0	41.0	32.0	41.0
21	37.94045456838706	40.0	36.0	41.0	33.0	41.0
22	37.9284723273296	40.0	36.0	41.0	33.0	41.0
23	37.76690126633369	40.0	36.0	41.0	33.0	41.0
24	37.75893307603047	40.0	36.0	41.0	33.0	41.0
25	37.80982606831139	40.0	36.0	41.0	33.0	41.0
26	37.701292190101405	40.0	36.0	41.0	33.0	41.0
27	37.512404772715804	40.0	35.0	41.0	33.0	41.0
28	37.42073116896221	40.0	35.0	41.0	32.0	41.0
29	37.455151733010446	40.0	35.0	41.0	32.0	41.0
30	37.30691879824429	40.0	35.0	41.0	32.0	41.0
31	37.36884491196206	40.0	35.0	41.0	32.0	41.0
32	37.04941097825539	40.0	35.0	41.0	31.0	41.0
33	36.858026209575705	40.0	35.0	41.0	31.0	41.0
34	36.73870200797134	40.0	35.0	41.0	31.0	41.0
35	36.650027117703445	40.0	35.0	41.0	30.0	41.0
36	36.65691690631149	39.0	35.0	41.0	30.0	41.0
37	36.6395458099995	39.0	35.0	41.0	31.0	41.0
38	36.60375674789365	39.0	35.0	41.0	30.0	41.0
39	36.40339475808486	39.0	35.0	41.0	30.0	41.0
40	36.24396473437263	39.0	35.0	41.0	30.0	41.0
41	36.16144808536401	39.0	35.0	41.0	29.0	41.0
42	36.254121260279504	39.0	35.0	41.0	30.0	41.0
43	36.15414837798295	39.0	35.0	41.0	30.0	41.0
44	36.248565284294436	39.0	35.0	41.0	30.0	41.0
45	36.173546995610714	39.0	35.0	41.0	30.0	41.0
46	36.00923263205691	39.0	35.0	41.0	29.0	41.0
47	35.78009749760355	38.0	35.0	41.0	28.0	41.0
48	35.85930011099339	38.0	35.0	40.0	29.0	41.0
49	35.824766030977244	38.0	35.0	41.0	29.0	41.0
50	35.694913223348976	38.0	35.0	40.0	28.0	41.0
51	34.94914169315373	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	4.0
12	9.0
13	7.0
14	6.0
15	14.0
16	20.0
17	35.0
18	60.0
19	136.0
20	404.0
21	830.0
22	1614.0
23	2107.0
24	2563.0
25	2925.0
26	3342.0
27	3424.0
28	3517.0
29	3786.0
30	4760.0
31	6005.0
32	7896.0
33	10564.0
34	18345.0
35	26689.0
36	22418.0
37	32386.0
38	55952.0
39	107187.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.62233237475405	15.303213763180464	17.86016094041673	15.214292921648756
2	26.30701024166288	35.39522476161647	20.52652489783563	17.771240098885023
3	27.318878966752436	35.217698400686146	22.56098330053983	14.902439332021594
4	24.579990918722565	20.78729630190202	37.09954089097422	17.533171888401192
5	33.46040815296907	23.756369507088444	22.30620301700217	20.477019322940315
6	21.99214217244337	38.875435144543665	24.28327279148378	14.849149891529187
7	65.88876696433076	5.767241814237424	22.153271782452954	6.190719438978861
8	63.15902325816054	16.211026184349933	12.550136219161494	8.079814338328035
9	58.79086574844862	7.10988345693961	13.269386004742445	20.829864789869333
10	41.46233792442359	21.11018616618738	20.56814741940366	16.85932848998537
11	34.41394228343676	20.83711719893043	25.233338378487463	19.51560213914535
12	25.373341405579943	18.363730386963322	29.252749609000556	27.01017859845618
13	26.09038393622925	21.92592452449422	34.72327329599919	17.260418243277332
14	21.393030119570152	30.07321779930377	26.445751980222997	22.08800010090308
15	17.63785883658746	20.84626154079007	38.77453206195449	22.74134756066798
16	19.782679985873568	22.92864890772413	27.652805105695975	29.635866000706322
17	20.157913324252057	25.437351798597447	30.877604560819332	23.527130316331164
18	20.883154230361736	21.669882952424196	30.157408808839108	27.289554008374957
19	20.63972554361536	25.15923767721104	26.810895010342566	27.39014176883104
20	24.334985621310732	24.22966802885828	31.82388628222592	19.611460067605066
21	21.807048080318854	31.61262045305484	27.31793300035316	19.262398466273144
22	19.711732505928055	21.301902023106805	32.15528984410474	26.8310756268604
23	21.94673578527824	28.172771303163312	28.126734271732	21.753758639826444
24	22.897116694415015	24.159666515312043	26.72481206800868	26.218404722264268
25	18.56364461934312	32.34038393622925	25.239014176883103	23.856957267544523
26	18.275440189697793	22.00570102416629	31.29509106503204	28.423767721103882
27	24.671119015185912	24.67837142424701	24.566116744866555	26.084392815700518
28	16.664144089601937	25.67132082135109	30.974408455678322	26.69012663336865
29	22.40679077745825	20.537245850360726	27.15901064527521	29.896952726905806
30	19.922367690832953	28.240880883911	27.05810756268604	24.77864386257
31	24.33277836637909	22.400169012663337	21.38451642197669	31.882536198980876
32	28.137455224257103	25.583030624085566	23.06676000201806	23.212754149639274
33	22.582109883456937	25.796819030321377	21.526096059734627	30.09497502648706
34	22.32985217698401	25.520912163866605	24.40561777912315	27.743617880026232
35	21.322397961757733	23.389965188436506	26.932924675848845	28.354712173956916
36	19.053970536299882	30.399260884920032	21.988988951112457	28.557779627667625
37	21.450734069925836	23.847182281418696	27.36901518591393	27.33306846274154
38	19.47145704051259	27.97191110438424	22.50737853791433	30.04925331718884
39	25.82362141163412	28.492507946117758	23.5829423338883	22.100928308359823
40	20.614499772968063	25.41906311487816	29.19252308158014	24.773914030573636
41	18.19061853589627	31.52874476565259	22.761528177185813	27.519108521265323
42	20.531570051965087	21.83006659603451	32.28804046213612	25.350322889864284
43	22.89680137228192	25.553390343574993	24.321742091720903	27.22806619242218
44	21.57402502396448	22.01642197669139	28.6299883961455	27.779564603198626
45	20.646662630543364	20.404810554462436	26.394354472529137	32.55417234246506
46	28.13997780132183	24.56012562433782	24.77013016497654	22.529766409363805
47	17.326320569093387	24.517241814237426	35.02440593310126	23.132031683567934
48	21.34037132334393	25.42190101407598	25.700961101861665	27.53676656071843
49	21.275099641794057	19.27280409666515	35.185220220977754	24.26687604056304
50	22.821754704606224	21.282352050855152	29.409149387013773	26.486743857524846
51	20.352467080369305	20.28688007668634	26.10583472075072	33.25481812219363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	226.5
2	359.0
3	1053.5
4	1748.0
5	1192.5
6	637.0
7	629.0
8	621.0
9	614.0
10	607.0
11	604.0
12	601.0
13	617.0
14	633.0
15	599.0
16	565.0
17	580.5
18	596.0
19	583.5
20	571.0
21	716.5
22	862.0
23	862.0
24	862.0
25	964.0
26	1242.0
27	1418.0
28	1858.5
29	2299.0
30	2712.0
31	3125.0
32	3478.0
33	3831.0
34	4311.5
35	4792.0
36	5407.0
37	6022.0
38	6573.5
39	7125.0
40	8228.5
41	9332.0
42	11000.0
43	12668.0
44	15186.0
45	17704.0
46	36500.5
47	55297.0
48	43792.5
49	32288.0
50	33054.5
51	33821.0
52	29628.5
53	25436.0
54	23247.0
55	21058.0
56	18617.0
57	16176.0
58	14465.0
59	12754.0
60	11724.5
61	10695.0
62	9876.5
63	9058.0
64	7640.0
65	6222.0
66	5599.0
67	4976.0
68	4282.0
69	3588.0
70	3044.5
71	2501.0
72	2221.5
73	1942.0
74	1589.0
75	1117.5
76	999.0
77	734.5
78	470.0
79	382.5
80	295.0
81	202.0
82	109.0
83	81.0
84	53.0
85	35.0
86	17.0
87	9.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.613572828981916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05411945556541	23.173286996632637
2	10.719409589485933	6.991964906607732
3	4.03533953891422	3.948205198261477
4	2.2289277712613873	2.9077319279429443
5	1.5326223413335895	2.49921451742039
6	1.1571811015024964	2.264388607810391
7	0.9784596528509163	2.2337745603931602
8	0.9185536970972703	2.3965854318089934
9	0.813718736744474	2.388444775481076
>10	6.458830042232987	35.18617101386871
>50	0.06589624095870665	1.462698552523309
>100	0.024960697332843427	1.8244657145565673
>500	0.001996855786627474	0.5148099758547087
>1k	0.00698899525319616	3.981718143422756
>5k	0.0029952836799412115	8.226539677415156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	9826	3.0983552797537963	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	8009	2.525414963927148	RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	7429	2.3425281267342717	No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	2990	0.9428131779425861	RNA PCR Primer, Index 40 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT	2210	0.6968619141314767	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT	1638	0.5164976540033298	RNA PCR Primer, Index 46 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT	1516	0.4780283537662075	TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTA	1424	0.4490187175218203	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC	1387	0.43735179859744716	No Hit
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1063	0.3351874274759094	RNA PCR Primer, Index 46 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.27874476565259065	No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	697	0.21977952676454265	RNA PCR Primer, Index 46 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATG	495	0.15608445588012715	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT	382	0.12045305484082539	RNA PCR Primer, Index 46 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	361	0.1138312900459109	RNA PCR Primer, Index 40 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCGAATA	352	0.1109933908480904	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCGAATATCGT	351	0.11067806871499924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2286085464910953	0.0
2	0.0	0.0	0.0	1.7617047575803442	0.0
3	0.0	0.0	0.0	2.1728848191312244	0.0
4	0.0	0.0	0.0	3.1267342717320012	0.0
5	0.0	0.0	0.0	6.886004742444881	0.0
6	0.0	0.0	0.0	7.8849452600776955	0.0
7	0.0	0.0	0.0	9.258173149689723	0.0
8	0.0	0.0	0.0	11.164295444225822	0.0
9	0.0	0.0	0.0	11.814489682659806	0.0
10	0.0	0.0	0.0	15.379521719388528	0.0
11	0.0	0.0	0.0	16.7726149033853	0.0
12	0.0	0.0	0.0	21.353614852933756	0.0
13	0.0	0.0	0.0	22.077279148377983	0.0
14	0.0	0.0	0.0	22.41152060945462	0.0
15	0.0	0.0	0.0	23.246493617880027	0.0
16	0.0	0.0	0.0	24.11898996014328	0.0
17	0.0	0.0	0.0	25.01608142878765	0.0
18	0.0	0.0	0.0	25.996417940568083	0.0
19	0.0	0.0	0.0	27.294914484637506	0.0
20	0.0	0.0	0.0	27.96875788305333	0.0
21	0.0	0.0	0.0	28.621159376418948	0.0
22	0.0	0.0	0.0	29.27860602391403	0.0
23	3.1532213309116595E-4	0.0	0.0	29.90262852530145	0.0
24	3.1532213309116595E-4	0.0	0.0	30.396422985722214	0.0
25	3.1532213309116595E-4	0.0	0.0	30.8359820392513	0.0
26	3.1532213309116595E-4	0.0	0.0	31.283108823974572	0.0
27	3.1532213309116595E-4	0.0	0.0	31.833030624085566	0.0
28	3.1532213309116595E-4	0.0	0.0	32.26943645628374	0.0
29	3.1532213309116595E-4	0.0	0.0	32.725392260733564	0.0
30	3.1532213309116595E-4	0.0	0.0	33.216979466222696	0.0
31	3.1532213309116595E-4	0.0	0.0	33.63288935976994	0.0
32	3.1532213309116595E-4	0.0	0.0	34.053529085313556	0.0
33	3.1532213309116595E-4	0.0	0.0	34.496241360173556	0.0
34	3.1532213309116595E-4	0.0	0.0	34.930124615307	0.0
35	3.1532213309116595E-4	0.0	0.0	35.38008929922809	0.0
36	3.1532213309116595E-4	0.0	0.0	35.770773422128045	0.0
37	3.1532213309116595E-4	0.0	0.0	36.197404268200394	0.0
38	3.1532213309116595E-4	0.0	0.0	36.67417133343424	0.0
39	3.1532213309116595E-4	0.0	0.0	37.161028706927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGG	20	7.025553E-4	45.0	2
GAAAACG	20	7.025553E-4	45.0	1
TCGTAAG	20	7.025553E-4	45.0	42
TAGGACG	20	7.025553E-4	45.0	1
ATTGCGG	25	3.8843973E-5	45.0	2
TAGTGCG	25	3.8843973E-5	45.0	1
GCATACG	25	3.8843973E-5	45.0	1
ACGGCTA	90	0.0	42.5	30
CGGCTAT	80	0.0	42.1875	31
ATCGAAT	130	0.0	41.538464	43
GATGAAT	880	0.0	41.420452	20
TCGAATG	120	0.0	41.250004	44
CGATGAA	815	0.0	41.13497	19
CGTTTTT	615	0.0	40.97561	1
CGACGGG	50	1.0768417E-9	40.5	3
CGTAAGG	45	1.920671E-8	40.0	2
ACGGCTG	1165	0.0	39.785408	8
ATGAATG	905	0.0	39.779007	21
CGAATGC	125	0.0	39.600002	45
CCGATGA	820	0.0	39.512196	18
>>END_MODULE