FastQCFastQC Report
Sat 18 Jun 2016
SRR3554413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320197
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC107313.3513743101902893No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC96653.018454264093667Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG89812.804835772977261No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC28730.8972601242360172Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT28190.8803955065163009No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT19440.6071262379097868Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT17400.5434154598575252Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA17120.5346708432621168No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC16250.5075000702692405Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.35072158702298895No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC9560.29856619518608857Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT7390.23079541657167307Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG5510.17208156228821633No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT5080.15865232965955334Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGAGCATC4670.14584771250199097No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGT3970.12398617101346983No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTAT3810.118989247244665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGC207.025655E-445.031
CGACAAA750.045.012
TTGCGAG351.2082637E-745.01
CGAATAC253.884481E-545.045
CGTAAGC351.2082637E-745.043
CCTATCC253.884481E-545.033
ATACCGC207.025655E-445.028
GCGTAAG207.025655E-445.01
AAACGGG600.044.9999963
TCGAATA302.1605792E-644.99999644
CGATGAA14150.044.2049519
ATGAATG14800.043.93581421
CCGATGA13450.043.82899518
GATGAAT15150.043.5148520
TGAATGA15150.042.77227422
AACGATG850.042.3529417
CAAACGA800.042.187515
ATACGGA750.042.028
CTCGAAT2150.041.86046643
ACGGAGA700.041.78571330
Produced by FastQC (version 0.11.5)