##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320197
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.885495491837837	33.0	31.0	34.0	30.0	34.0
2	31.97004031892866	33.0	31.0	34.0	30.0	34.0
3	32.05906676202463	33.0	31.0	34.0	30.0	34.0
4	35.695890342507894	37.0	35.0	37.0	35.0	37.0
5	35.70336386661961	37.0	35.0	37.0	35.0	37.0
6	35.70374488205698	37.0	35.0	37.0	35.0	37.0
7	35.8484745328657	37.0	35.0	37.0	35.0	37.0
8	35.49014200632735	37.0	35.0	37.0	33.0	37.0
9	37.33289506147778	39.0	37.0	39.0	34.0	39.0
10	37.230717339637785	39.0	37.0	39.0	34.0	39.0
11	37.17565123970556	39.0	37.0	39.0	34.0	39.0
12	36.99604930714529	39.0	37.0	39.0	33.0	39.0
13	36.79017917094789	39.0	35.0	39.0	32.0	39.0
14	37.95943747130673	40.0	37.0	41.0	33.0	41.0
15	37.89273166207054	40.0	37.0	41.0	33.0	41.0
16	38.20584515157856	40.0	37.0	41.0	34.0	41.0
17	37.97899418170689	40.0	36.0	41.0	33.0	41.0
18	38.025415603519086	40.0	37.0	41.0	33.0	41.0
19	37.98702361358789	40.0	37.0	41.0	33.0	41.0
20	37.4803480357405	40.0	35.0	41.0	32.0	41.0
21	37.798442833630546	40.0	36.0	41.0	33.0	41.0
22	37.7191354072649	40.0	36.0	41.0	33.0	41.0
23	37.44190295349426	40.0	35.0	41.0	32.0	41.0
24	37.581314003566554	40.0	35.0	41.0	33.0	41.0
25	37.713220298753576	40.0	35.0	41.0	33.0	41.0
26	37.43525079872703	40.0	35.0	41.0	32.0	41.0
27	37.21560164523715	40.0	35.0	41.0	32.0	41.0
28	37.13769335752677	39.0	35.0	41.0	31.0	41.0
29	37.09900155216945	39.0	35.0	41.0	31.0	41.0
30	37.04314219058892	39.0	35.0	41.0	31.0	41.0
31	36.8793430294475	39.0	35.0	41.0	30.0	41.0
32	36.58808171219593	39.0	35.0	41.0	30.0	41.0
33	36.38605608422315	39.0	35.0	41.0	30.0	41.0
34	36.05771446952969	39.0	35.0	41.0	28.0	41.0
35	36.09617829024007	39.0	35.0	41.0	29.0	41.0
36	36.1458539586567	39.0	35.0	41.0	29.0	41.0
37	35.79498246392065	39.0	35.0	41.0	27.0	41.0
38	35.801825126406555	39.0	35.0	41.0	27.0	41.0
39	35.70876991352199	39.0	35.0	41.0	27.0	41.0
40	35.74523184164749	39.0	35.0	41.0	26.0	41.0
41	35.557047692514296	39.0	35.0	41.0	26.0	41.0
42	35.484329959368765	39.0	35.0	41.0	26.0	41.0
43	35.41008191831904	38.0	35.0	41.0	26.0	41.0
44	35.51280930177359	38.0	35.0	41.0	26.0	41.0
45	35.483399282316824	38.0	35.0	41.0	26.0	41.0
46	35.328956860932486	38.0	35.0	40.0	26.0	41.0
47	35.009706524420906	38.0	35.0	40.0	25.0	41.0
48	35.123961186394624	38.0	35.0	40.0	25.0	41.0
49	35.149901466909434	38.0	35.0	40.0	26.0	41.0
50	34.92201363535573	37.0	35.0	40.0	25.0	41.0
51	34.19789067355409	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	5.0
13	4.0
14	6.0
15	16.0
16	18.0
17	53.0
18	76.0
19	238.0
20	690.0
21	1665.0
22	2684.0
23	3360.0
24	3562.0
25	4002.0
26	4099.0
27	3806.0
28	3659.0
29	3878.0
30	4655.0
31	6115.0
32	8127.0
33	10911.0
34	18903.0
35	28255.0
36	23189.0
37	32685.0
38	52856.0
39	102544.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.117914908634376	14.581960480579143	17.60010243693726	15.700022173849224
2	25.014600386636975	39.17307157780991	20.137915096019015	15.674412939534099
3	25.94434051537022	38.30548068845117	21.907138417911472	13.84304037826713
4	24.622654178521348	19.35964421902766	39.38544083798411	16.632260764466874
5	35.77828649237813	23.1229524324088	21.39807680896448	19.70068426624859
6	21.442736815148177	41.80145348020125	22.548618506731792	14.207191197918782
7	63.89441500076516	5.3538915105388245	24.823780360215743	5.927913128480279
8	61.13017923340943	19.3499626792256	12.155953990824399	7.363904096540567
9	57.10515713763714	6.672142462296648	12.434844798670818	23.787855601395393
10	44.87581082895842	19.435847306501934	19.467078080056964	16.22126378448268
11	37.84763754813443	19.76626889071415	23.170423208212444	19.21567035293897
12	26.422171350762184	16.759057705100297	27.779148461728248	29.039622482409268
13	28.657357814095697	19.54640424488674	34.32699244527588	17.469245495741685
14	21.409632195179842	31.58087052658207	24.49086031411912	22.518636964118965
15	17.054500822930883	19.51298731718286	38.70148689712896	24.731024962757303
16	19.106362645496368	21.916195342242432	26.720112930477175	32.25732908178402
17	19.31654575152172	26.407492887191324	30.394413439226476	23.88154792206048
18	20.14384894299447	21.60544914536988	28.99964709225883	29.25105481937682
19	20.966155210698414	24.228209508521317	25.127343479170634	29.678291801609635
20	27.278831469376662	23.285977070366055	31.489676667801387	17.945514792455892
21	22.267853852472072	32.94534302320134	25.672320477705913	19.114482646620672
22	19.718173499439406	20.68133055587654	32.461266033098376	27.139229911585684
23	24.362189527072395	27.527740734610255	27.12642529442812	20.98364444388923
24	23.56049556991477	24.64232956586102	24.618281870223644	27.17889299400057
25	18.821537990674493	34.76765866013735	23.348126309740564	23.06267703944759
26	19.08824879683445	22.570480048220315	31.643332073692132	26.697939081253104
27	28.380965468133677	25.046143467927557	23.338757077674057	23.234133986264705
28	17.543262429067106	29.76886104491922	29.66298872256767	23.024887803446003
29	24.261938743960748	24.176678732155516	27.695137680865216	23.866244843018517
30	24.271932591498356	29.767924121712568	25.176375793652035	20.783767493137038
31	25.030840388885593	26.49899905370756	19.843096593659528	28.627063963747318
32	32.53434604321714	27.155782221569847	21.284709100959724	19.025162634253288
33	26.325355952741592	22.380284637270183	19.546716552622293	31.747642857365932
34	23.859686380571958	29.681727186700684	22.112949215639123	24.345637217088232
35	27.516185348394895	21.249730634578086	25.931535898212665	25.302548118814354
36	20.915249049803712	34.262032436281416	20.112305861703888	24.710412652210984
37	27.766343844570684	23.597660190445257	26.947160654222245	21.68883531076181
38	20.913999818861516	26.91218218784061	20.97552444276492	31.198293550532952
39	27.04397605224284	27.33504686177572	21.98646458274125	23.63451250324019
40	21.54111375184652	20.479267450975495	28.39377008529124	29.585848711886747
41	18.760325674506632	28.33099623044563	22.232875386090438	30.6758027089573
42	22.39277694669219	20.80906441971661	32.50779988569537	24.290358747895826
43	24.028020250033574	22.36966617426147	22.67885083245627	30.923462743248685
44	24.060187946795253	21.474904511909855	28.028682342432944	26.43622519886195
45	21.07608753361212	20.914624434332612	26.517112902369476	31.49217512968579
46	29.9518733779517	24.86094498074623	22.768170844823654	22.419010796478418
47	18.896179539471014	23.40340477893297	34.24735397271055	23.453061708885468
48	22.196023073295503	25.248831188299704	24.612348023248188	27.94279771515661
49	22.014884586676327	18.78874567844171	34.88914636926642	24.307223365615542
50	23.274421684150695	20.111368938497236	28.304137765188308	28.31007161216376
51	20.83842134685834	20.209121259724483	25.88656358429342	33.06589380912376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	192.5
2	336.0
3	1266.5
4	2197.0
5	1528.0
6	859.0
7	814.0
8	769.0
9	757.0
10	745.0
11	720.5
12	696.0
13	675.5
14	655.0
15	650.5
16	646.0
17	642.0
18	638.0
19	658.0
20	678.0
21	734.0
22	790.0
23	773.0
24	756.0
25	891.5
26	1278.0
27	1529.0
28	1704.5
29	1880.0
30	2080.5
31	2281.0
32	2631.0
33	2981.0
34	3756.0
35	4531.0
36	5024.5
37	5518.0
38	5771.5
39	6025.0
40	7429.0
41	8833.0
42	10540.5
43	12248.0
44	14106.0
45	15964.0
46	37353.0
47	58742.0
48	44563.5
49	30385.0
50	30688.0
51	30991.0
52	27077.0
53	23163.0
54	21442.5
55	19722.0
56	18051.0
57	16380.0
58	15225.0
59	14070.0
60	12938.5
61	11807.0
62	10937.5
63	10068.0
64	9108.5
65	8149.0
66	7129.0
67	6109.0
68	5380.5
69	4652.0
70	4260.0
71	3868.0
72	3614.0
73	3360.0
74	2841.0
75	1945.5
76	1569.0
77	1328.5
78	1088.0
79	852.5
80	617.0
81	448.0
82	279.0
83	215.5
84	152.0
85	103.0
86	54.0
87	31.5
88	9.0
89	6.5
90	4.0
91	5.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.25441916193796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16839113636235	22.954951187929815
2	10.014755263127372	6.460402281222691
3	3.8233779163140436	3.6996250178187036
4	2.3252826579529975	3.000025660784048
5	1.6927803049993522	2.7299822753261145
6	1.361193247753447	2.634269854406361
7	1.149472193844961	2.5952890568687446
8	1.036621976381292	2.6748511790942997
9	0.9377533849378273	2.7222021673479757
>10	6.385511316480826	32.606512341835185
>50	0.06391503350584722	1.3805890966064744
>100	0.026964154760279295	1.734594560248685
>500	0.003994689594115451	0.8871074129851214
>1k	0.00699070678970204	4.4567967197030285
>5k	0.0019973447970577257	6.006174590602968
>10k+	9.986723985288628E-4	3.456626597219803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	10731	3.3513743101902893	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	9665	3.018454264093667	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	8981	2.804835772977261	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2873	0.8972601242360172	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	2819	0.8803955065163009	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1944	0.6071262379097868	Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	1740	0.5434154598575252	Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	1712	0.5346708432621168	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	1625	0.5075000702692405	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.35072158702298895	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	956	0.29856619518608857	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	739	0.23079541657167307	Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG	551	0.17208156228821633	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	508	0.15865232965955334	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGAGCATC	467	0.14584771250199097	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGT	397	0.12398617101346983	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTAT	381	0.118989247244665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24141387958038332	0.0
2	0.0	0.0	0.0	1.6639756150120082	0.0
3	0.0	0.0	0.0	2.0256279727792577	0.0
4	0.0	0.0	0.0	2.939752714734991	0.0
5	0.0	0.0	0.0	7.0231763570551875	0.0
6	0.0	0.0	0.0	7.932304175242116	0.0
7	0.0	0.0	0.0	9.288656670737078	0.0
8	0.0	0.0	0.0	11.025087680396755	0.0
9	0.0	0.0	0.0	11.586616988916198	0.0
10	0.0	0.0	0.0	15.462980602566544	0.0
11	0.0	0.0	0.0	16.656620767839797	0.0
12	0.0	0.0	0.0	21.342173724300977	0.0
13	0.0	0.0	0.0	22.040181513255902	0.0
14	0.0	0.0	0.0	22.34718001730185	0.0
15	0.0	0.0	0.0	23.215707829867238	0.0
16	0.0	0.0	0.0	23.94244793049279	0.0
17	0.0	0.0	0.0	24.69698341958232	0.0
18	0.0	0.0	0.0	25.497740453533293	0.0
19	0.0	0.0	0.0	26.918428342551618	0.0
20	0.0	0.0	0.0	27.530551504230207	0.0
21	0.0	0.0	0.0	28.045234652417104	0.0
22	0.0	0.0	0.0	28.62862550242507	0.0
23	0.0	0.0	0.0	29.11582556988354	0.0
24	0.0	0.0	0.0	29.520888702892282	0.0
25	0.0	0.0	0.0	29.883477983866182	0.0
26	0.0	0.0	0.0	30.22451803108711	0.0
27	0.0	0.0	0.0	30.675490401221747	0.0
28	0.0	0.0	0.0	31.033395066162395	0.0
29	0.0	0.0	0.0	31.376933575267724	0.0
30	0.0	0.0	0.0	31.79761209505398	0.0
31	3.123077355503018E-4	0.0	0.0	32.18050137883865	0.0
32	3.123077355503018E-4	0.0	0.0	32.553084507350164	0.0
33	6.246154711006036E-4	0.0	0.0	32.91223840323301	0.0
34	6.246154711006036E-4	0.0	0.0	33.28825691683557	0.0
35	6.246154711006036E-4	0.0	0.0	33.661152353082635	0.0
36	6.246154711006036E-4	0.0	0.0	34.02936317329644	0.0
37	6.246154711006036E-4	0.0	0.0	34.401946301807946	0.0
38	6.246154711006036E-4	0.0	0.0	34.78327404691487	0.0
39	6.246154711006036E-4	0.0	0.0	35.19364641142796	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGC	20	7.025655E-4	45.0	31
CGACAAA	75	0.0	45.0	12
TTGCGAG	35	1.2082637E-7	45.0	1
CGAATAC	25	3.884481E-5	45.0	45
CGTAAGC	35	1.2082637E-7	45.0	43
CCTATCC	25	3.884481E-5	45.0	33
ATACCGC	20	7.025655E-4	45.0	28
GCGTAAG	20	7.025655E-4	45.0	1
AAACGGG	60	0.0	44.999996	3
TCGAATA	30	2.1605792E-6	44.999996	44
CGATGAA	1415	0.0	44.20495	19
ATGAATG	1480	0.0	43.935814	21
CCGATGA	1345	0.0	43.828995	18
GATGAAT	1515	0.0	43.51485	20
TGAATGA	1515	0.0	42.772274	22
AACGATG	85	0.0	42.35294	17
CAAACGA	80	0.0	42.1875	15
ATACGGA	75	0.0	42.0	28
CTCGAAT	215	0.0	41.860466	43
ACGGAGA	70	0.0	41.785713	30
>>END_MODULE