##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217568
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01774157964407	33.0	31.0	34.0	30.0	34.0
2	32.06812582732755	33.0	31.0	34.0	30.0	34.0
3	32.13057526842183	34.0	31.0	34.0	30.0	34.0
4	35.724624025592	37.0	35.0	37.0	33.0	37.0
5	35.837090013237244	37.0	35.0	37.0	35.0	37.0
6	35.86441939991175	37.0	35.0	37.0	35.0	37.0
7	36.07838468892484	37.0	35.0	37.0	35.0	37.0
8	35.96098231357553	37.0	35.0	37.0	35.0	37.0
9	37.73306276658332	39.0	38.0	39.0	35.0	39.0
10	37.517819716134724	39.0	37.0	39.0	35.0	39.0
11	37.47733582144433	39.0	37.0	39.0	35.0	39.0
12	37.32085141197235	39.0	37.0	39.0	35.0	39.0
13	37.195589424915426	39.0	37.0	39.0	34.0	39.0
14	38.312164472716574	40.0	38.0	41.0	34.0	41.0
15	38.46230144138844	40.0	38.0	41.0	34.0	41.0
16	38.57674841888513	40.0	38.0	41.0	35.0	41.0
17	38.57657835711134	40.0	38.0	41.0	35.0	41.0
18	38.53132813649066	40.0	38.0	41.0	35.0	41.0
19	38.502918627739376	40.0	37.0	41.0	35.0	41.0
20	38.40021050889837	40.0	37.0	41.0	34.0	41.0
21	38.35194054272687	40.0	37.0	41.0	34.0	41.0
22	38.3809475658185	40.0	37.0	41.0	34.0	41.0
23	38.370210692748934	40.0	37.0	41.0	34.0	41.0
24	38.31548757170172	40.0	37.0	41.0	34.0	41.0
25	38.19370036034711	40.0	36.0	41.0	34.0	41.0
26	38.200452272393	40.0	37.0	41.0	34.0	41.0
27	38.17500275775849	40.0	36.0	41.0	34.0	41.0
28	38.10065818502721	40.0	36.0	41.0	34.0	41.0
29	38.14401014855126	40.0	36.0	41.0	34.0	41.0
30	37.99043057802618	40.0	36.0	41.0	34.0	41.0
31	37.921808354169734	40.0	36.0	41.0	34.0	41.0
32	37.86794473451978	40.0	36.0	41.0	34.0	41.0
33	37.80177231945874	40.0	36.0	41.0	34.0	41.0
34	37.63452805559641	40.0	36.0	41.0	33.0	41.0
35	37.62317068686571	40.0	36.0	41.0	33.0	41.0
36	37.525132372407704	40.0	36.0	41.0	33.0	41.0
37	37.46123970436829	40.0	35.0	41.0	33.0	41.0
38	37.37831390645683	40.0	35.0	41.0	33.0	41.0
39	37.35228985880276	40.0	35.0	41.0	33.0	41.0
40	37.23089792616561	40.0	35.0	41.0	33.0	41.0
41	37.19102533460803	40.0	35.0	41.0	33.0	41.0
42	37.18609813943227	40.0	35.0	41.0	33.0	41.0
43	37.02892888660097	39.0	35.0	41.0	32.0	41.0
44	36.96318392410649	39.0	35.0	41.0	32.0	41.0
45	36.946329423444624	39.0	35.0	41.0	32.0	41.0
46	36.87561589939697	39.0	35.0	41.0	32.0	41.0
47	36.82586133990293	39.0	35.0	41.0	32.0	41.0
48	36.77125312545962	39.0	35.0	41.0	32.0	41.0
49	36.70094407265774	39.0	35.0	41.0	32.0	41.0
50	36.56697676128842	39.0	35.0	41.0	31.0	41.0
51	35.78296900279453	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	2.0
12	2.0
13	2.0
14	4.0
15	4.0
16	17.0
17	32.0
18	46.0
19	71.0
20	101.0
21	164.0
22	309.0
23	453.0
24	697.0
25	1026.0
26	1467.0
27	1755.0
28	1991.0
29	2181.0
30	2612.0
31	3314.0
32	4301.0
33	6310.0
34	12262.0
35	19188.0
36	14277.0
37	21217.0
38	39620.0
39	84022.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52768789527872	20.22356228857185	23.021308280629505	12.22744153551993
2	34.458192381232536	23.4207236358288	23.61928224738932	18.501801735549346
3	31.727092219444035	23.919877923224004	26.39450654508016	17.9585233122518
4	30.04485953816738	25.22062067951169	25.468359317546696	19.26616046477423
5	25.233490219149875	28.528092366524486	25.882023091631122	20.356394322694513
6	25.662321664950728	31.967476834828652	26.62477937932049	15.745422120900132
7	75.5418995440506	6.270683188704221	12.162634210913371	6.024783056331814
8	74.5771436976026	5.665814825709663	11.635442712163552	8.121598764524194
9	67.46258640976615	7.80123915281659	15.436093543168115	9.300080894249154
10	38.12095528754229	23.710288277687898	22.25005515516988	15.918701279599942
11	30.07059861744374	24.194734519782322	26.898257096631855	18.83640976614208
12	25.981302397411383	22.03311148698338	32.194991910575084	19.79059420503015
13	23.359133696131785	26.61420797176055	32.46387336372996	17.562784968377702
14	20.168866745109575	28.321260479482273	31.043168112957787	20.46670466245036
15	17.896933372554788	25.56396161200177	37.33499411678188	19.20411089866157
16	21.11385865568466	24.26183997646713	32.96164877187822	21.662652595969995
17	20.681809824974263	23.87345565524342	29.581096484777174	25.86363803500515
18	22.014726430357406	23.503916017061332	33.91951022209149	20.561847330489776
19	22.074477864391824	25.557526842182675	30.19929401382556	22.168701279599944
20	24.061442859244007	25.7570047065745	29.824698485071334	20.356853949110164
21	22.04230401529637	27.78487645241947	30.15241211942933	20.020407412854833
22	20.399599205765554	25.33598690983968	28.676092072363584	25.58832181203118
23	19.867351816443595	27.14691498749816	29.370127959994118	23.615605236064127
24	21.21589571995882	24.125330931019267	31.733986615678777	22.92478673334314
25	18.455839093984412	26.134357993822622	30.172176055302252	25.23762685689072
26	18.57901897337844	27.02465436093543	29.9239777908516	24.472348874834534
27	22.204552140020592	26.454257979114576	28.94865053684365	22.392539344021177
28	17.835803059273424	27.232865127224592	30.98433593175467	23.946995881747316
29	19.735898661567877	24.00123179879394	31.684346962788645	24.578522576849537
30	22.278092366524486	24.991726724518312	30.070138991028095	22.660041917929107
31	22.215123547580525	28.34332254743345	25.873290189733783	23.568263715252243
32	21.809273422562143	26.78059273422562	27.209883806442125	24.200250036770115
33	23.718561553169586	24.87727974702162	27.89426753934402	23.509891160464775
34	19.07173849095455	26.414270480953085	30.40980291219297	24.104188115899397
35	20.348121047212825	23.956188410060303	29.865605236064123	25.830085306662742
36	24.26000147080453	25.705986174437417	27.999062362112078	22.034949992645977
37	21.211299455802322	28.315285336078833	29.543407118693928	20.930008089424916
38	19.165042653331373	28.631967936461244	28.369980879541107	23.833008530666273
39	22.524911751728197	24.744907339314608	30.413939549933815	22.316241359023387
40	22.63476246506839	23.198264450654506	30.083927783497572	24.083045300779528
41	20.479114575672895	24.595069127812913	26.150444918370347	28.775371378143845
42	23.64410207383439	22.819991910575084	27.41441756140609	26.121488454184437
43	24.688373290189734	22.80252610678041	29.053904986027355	23.4551956170025
44	20.985163259302837	23.938262979849977	29.984648477717315	25.091925283129875
45	20.365127224591852	22.769433004853653	30.101853213707898	26.763586556846597
46	22.459185174290337	23.697878364465364	30.205269157229004	23.6376673040153
47	19.998804971319313	24.10464774231505	31.09924253566701	24.797304750698633
48	20.447859979408737	21.77480144138844	30.486560523606414	27.29077805559641
49	22.351172966612737	20.958964553610826	33.063685836152374	23.62617664362406
50	20.836703927048095	21.477882776878953	31.496819385203707	26.188593910869244
51	20.02959994116782	22.143881453154876	28.362167230475073	29.464351375202234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	162.0
2	252.0
3	900.5
4	1549.0
5	1018.5
6	488.0
7	500.5
8	513.0
9	508.5
10	504.0
11	511.0
12	518.0
13	514.0
14	510.0
15	509.5
16	509.0
17	517.0
18	525.0
19	512.5
20	500.0
21	553.0
22	606.0
23	742.0
24	878.0
25	1041.0
26	1589.0
27	1974.0
28	2117.5
29	2261.0
30	2589.0
31	2917.0
32	3343.0
33	3769.0
34	4091.5
35	4414.0
36	5282.0
37	6150.0
38	6319.0
39	6488.0
40	7733.0
41	8978.0
42	10418.5
43	11859.0
44	13669.5
45	15480.0
46	18291.5
47	21103.0
48	24699.5
49	28296.0
50	28308.0
51	28320.0
52	23114.5
53	17909.0
54	15349.0
55	12789.0
56	10797.0
57	8805.0
58	8208.5
59	7612.0
60	6518.0
61	5424.0
62	4936.5
63	4449.0
64	3881.0
65	3313.0
66	2778.0
67	2243.0
68	1848.0
69	1453.0
70	1158.5
71	864.0
72	848.0
73	832.0
74	696.5
75	471.0
76	381.0
77	298.0
78	215.0
79	136.5
80	58.0
81	33.5
82	9.0
83	8.5
84	8.0
85	6.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	217568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.249191057508455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57324136605061	27.75867774672746
2	11.200701771251412	8.568355640535373
3	4.1829892571318705	4.799878658626269
4	2.112523732846259	3.232092954846301
5	1.3374510322286044	2.5578210030886894
6	0.9529188396741088	2.186902485659656
7	0.7161912086327478	1.9175614060891306
8	0.6296714653079862	1.926753934402118
9	0.5611766685092168	1.931809824974261
>10	5.616573337499099	38.391215619944106
>50	0.0769064385108991	2.001213413737314
>100	0.03364656684851835	2.2415980291219295
>500	0.0036049893051983946	1.060358140903074
>1k	0.0024033262034655967	1.4257611413443152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1995	0.9169546992204736	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1107	0.5088064421238417	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.43112957787909983	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	847	0.3893035740550081	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	522	0.239924988968966	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	423	0.19442197381967938	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	314	0.14432269451389912	No Hit
GCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	282	0.12961464921311958	No Hit
GGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	252	0.11582585674363877	No Hit
GCCGCTGGTCTCCAATTACCAAGAATTCTCCAAAAATTAATTTTCTGCAGG	241	0.11076996617149582	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	229	0.1052544491837035	No Hit
AGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	226	0.1038755699367554	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40355199294013827	0.0
2	0.0	0.0	0.0	2.3372003235769965	0.0
3	0.0	0.0	0.0	3.18245330195617	0.0
4	0.0	0.0	0.0	4.142612884247684	0.0
5	0.0	0.0	0.0	6.4241984115311075	0.0
6	0.0	0.0	0.0	7.649562435652302	0.0
7	0.0	0.0	0.0	9.02338579202824	0.0
8	0.0	0.0	0.0	11.298076923076923	0.0
9	0.0	0.0	0.0	12.256857626121489	0.0
10	0.0	0.0	0.0	14.10271731136932	0.0
11	0.0	0.0	0.0	16.388899102809237	0.0
12	0.0	0.0	0.0	18.27520591263421	0.0
13	0.0	0.0	0.0	19.052893807912927	0.0
14	0.0	0.0	0.0	19.330508162965142	0.0
15	0.0	0.0	0.0	19.96295411089866	0.0
16	0.0	0.0	0.0	21.187858508604208	0.0
17	0.0	0.0	0.0	22.698650536843655	0.0
18	0.0	0.0	0.0	24.38364097661421	0.0
19	0.0	0.0	0.0	25.25876967201059	0.0
20	0.0	0.0	0.0	26.04978673334314	0.0
21	0.0	0.0	0.0	27.023735108104134	0.0
22	0.0	0.0	0.0	27.931956905427267	0.0
23	0.0	0.0	0.0	28.855346374466833	0.0
24	0.0	0.0	0.0	29.595344903662305	0.0
25	0.0	0.0	0.0	30.253989557287838	0.0
26	0.0	0.0	0.0	30.867131195764085	0.0
27	0.0	0.0	0.0	31.456831887042213	0.0
28	0.0	0.0	0.0	32.05756361229592	0.0
29	0.0	0.0	0.0	32.64220841300191	0.0
30	0.0	0.0	0.0	33.400132372407704	0.0
31	0.0	0.0	0.0	34.053721135461096	0.0
32	0.0	0.0	0.0	34.66226650978085	0.0
33	0.0	0.0	0.0	35.26575599352846	0.0
34	0.0	0.0	0.0	35.83109648477717	0.0
35	0.0	0.0	0.0	36.44607662891602	0.0
36	0.0	0.0	0.0	37.01417487865863	0.0
37	0.0	0.0	0.0	37.55791292837182	0.0
38	0.0	0.0	0.0	38.11681864980144	0.0
39	0.0	0.0	0.0	38.68767465803795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTC	30	2.157365E-6	45.000004	5
CGGGTCT	20	7.020667E-4	45.000004	6
TTTACGG	20	7.020667E-4	45.000004	2
CTATAGG	30	2.157365E-6	45.000004	2
GGCGTCC	40	6.7720975E-9	45.000004	6
CCAGCGG	20	7.020667E-4	45.000004	2
CGTCTAA	20	7.020667E-4	45.000004	13
TACGGGT	20	7.020667E-4	45.000004	4
CACTGCT	20	7.020667E-4	45.000004	16
GGATACG	20	7.020667E-4	45.000004	1
AGACGTC	20	7.020667E-4	45.000004	10
ACGTCTA	20	7.020667E-4	45.000004	12
GTTCACC	20	7.020667E-4	45.000004	34
TGCTAGT	20	7.020667E-4	45.000004	16
ACGTAGG	30	2.157365E-6	45.000004	2
CGGTCGC	20	7.020667E-4	45.000004	43
CAGTAGG	30	2.157365E-6	45.000004	2
AGGGCGT	20	7.020667E-4	45.000004	6
TCAAGCG	60	0.0	45.000004	17
GTAACCC	20	7.020667E-4	45.000004	13
>>END_MODULE