FastQCFastQC Report
Sat 18 Jun 2016
SRR3554410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778987
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81701.048797990210363No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC26730.34313794710309675No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC18640.23928512285827622No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC17960.2305558372604421TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG17830.22888700324909145No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG15410.19782101626856416No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT12300.15789737184317582No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC11720.15045180471561143TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT9710.12464906346319002Illumina PCR Primer Index 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC9520.12220999836967754No Hit
CGTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT8910.11437931570103224No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT8750.11232536614860068No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT8130.10436631163292841No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG207.031885E-445.02
CGTTTTT50250.043.835821
ACACGAC1600.040.7812526
CTCGATC501.0804797E-940.50000416
CACGACC1650.039.54545227
CTTGCGG1100.038.863642
TAACGGG2450.038.571433
ACGTAGG1050.038.571432
AATAGCG356.2456966E-638.5714261
GCGAGAC1700.038.3823521
TCAAGCG1700.038.3823517
CTAACGG659.094947E-1238.0769272
GTTTACG301.1395842E-437.4999961
TAGCGCG301.1395842E-437.4999961
TATGCGG1450.037.241382
TTAACGG850.037.0588232
CGTAAGG1100.036.8181842
TACGGCT3350.036.2686587
AACGTTG250.002106692436.0000041
TCCGAAT250.002106692436.00000416
Produced by FastQC (version 0.11.5)