##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778987
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.168731955732255	33.0	31.0	34.0	30.0	34.0
2	32.24046357641399	34.0	31.0	34.0	30.0	34.0
3	32.27971968723483	34.0	31.0	34.0	30.0	34.0
4	35.85098595997109	37.0	35.0	37.0	35.0	37.0
5	35.94205551568896	37.0	35.0	37.0	35.0	37.0
6	35.97420367733993	37.0	35.0	37.0	35.0	37.0
7	36.17097204446287	37.0	36.0	37.0	35.0	37.0
8	36.053240939835966	37.0	37.0	37.0	35.0	37.0
9	37.88393772938444	39.0	38.0	39.0	35.0	39.0
10	37.60366090833352	39.0	37.0	39.0	35.0	39.0
11	37.57208015024641	39.0	37.0	39.0	35.0	39.0
12	37.57655904398918	39.0	37.0	39.0	35.0	39.0
13	37.587931505917304	39.0	37.0	39.0	35.0	39.0
14	38.86230964059734	40.0	38.0	41.0	35.0	41.0
15	38.96989423443524	40.0	38.0	41.0	35.0	41.0
16	39.01383335023563	40.0	38.0	41.0	35.0	41.0
17	38.958612916518504	40.0	38.0	41.0	35.0	41.0
18	38.8185065989548	40.0	38.0	41.0	35.0	41.0
19	38.70205022676887	40.0	38.0	41.0	35.0	41.0
20	38.51373899692806	40.0	37.0	41.0	35.0	41.0
21	38.48669489991489	40.0	37.0	41.0	35.0	41.0
22	38.50814070067921	40.0	37.0	41.0	35.0	41.0
23	38.47330828370691	40.0	37.0	41.0	35.0	41.0
24	38.421010876946596	40.0	37.0	41.0	35.0	41.0
25	38.2996134723686	40.0	37.0	41.0	34.0	41.0
26	38.2673934224833	40.0	37.0	41.0	34.0	41.0
27	38.227958874795085	40.0	37.0	41.0	34.0	41.0
28	38.127177988849624	40.0	37.0	41.0	34.0	41.0
29	38.116601432373066	40.0	37.0	41.0	34.0	41.0
30	37.92618747167796	40.0	36.0	41.0	34.0	41.0
31	37.809521853381376	40.0	36.0	41.0	33.0	41.0
32	37.59859407153136	40.0	36.0	41.0	33.0	41.0
33	37.36763643038972	40.0	36.0	41.0	33.0	41.0
34	37.12131909775131	40.0	36.0	41.0	31.0	41.0
35	36.9966623319773	40.0	36.0	41.0	31.0	41.0
36	36.87765649490942	40.0	36.0	41.0	31.0	41.0
37	36.851427559124865	40.0	36.0	41.0	31.0	41.0
38	36.746528504326776	40.0	36.0	41.0	30.0	41.0
39	36.66092887301072	40.0	35.0	41.0	30.0	41.0
40	36.559244249262186	40.0	35.0	41.0	30.0	41.0
41	36.48404273755531	40.0	35.0	41.0	30.0	41.0
42	36.4462616192568	40.0	35.0	41.0	30.0	41.0
43	36.33101836102528	40.0	35.0	41.0	29.0	41.0
44	36.25816477040053	40.0	35.0	41.0	29.0	41.0
45	36.21196759381094	39.0	35.0	41.0	29.0	41.0
46	36.11695445495239	39.0	35.0	41.0	28.0	41.0
47	36.04742056029176	39.0	35.0	41.0	28.0	41.0
48	35.97551948877196	39.0	35.0	41.0	28.0	41.0
49	35.89025490797664	39.0	35.0	41.0	28.0	41.0
50	35.78739696554628	39.0	35.0	41.0	27.0	41.0
51	34.97215101150597	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	4.0
11	9.0
12	8.0
13	13.0
14	18.0
15	30.0
16	57.0
17	87.0
18	185.0
19	316.0
20	637.0
21	994.0
22	1610.0
23	2438.0
24	4092.0
25	6933.0
26	10399.0
27	12018.0
28	11501.0
29	10483.0
30	10731.0
31	12059.0
32	14399.0
33	18917.0
34	32399.0
35	46771.0
36	49377.0
37	71968.0
38	135547.0
39	324467.0
40	511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69231835704575	19.761690503179132	23.64481050389801	16.901180635877108
2	35.74013430262636	24.38346211169121	23.552126030344535	16.324277555337893
3	29.853514885357523	23.91323603603141	30.662385893474475	15.570863185136593
4	26.71020183905508	24.54367017678087	31.353411546020666	17.39271643814338
5	25.571543555925835	27.74603427271572	28.496367718588374	18.186054452770072
6	25.18219174389303	32.5239060472126	27.827293651883796	14.466608557010579
7	76.31757654492309	5.170432882705359	14.026549865402119	4.485440706969436
8	77.64070517223008	5.402914297671206	12.322156852425008	4.634223677673697
9	70.68667384693198	7.559818071418394	15.03221491501142	6.721293166638211
10	33.65896991862509	32.241616355600286	20.938218481181327	13.1611952445933
11	24.929299205249894	23.78435070161633	34.35667090721668	16.929679185917095
12	22.136569673178112	22.478552273657968	35.39648286813515	19.98839518502876
13	22.163656132900805	21.990739254955475	37.63708508614393	18.20851952599979
14	20.143853491778422	25.760635286596568	34.13933737020002	19.956173851424992
15	19.514703069499234	25.01107207180608	36.35978520822555	19.114439650469134
16	22.587154856242787	24.942264761799617	33.836251439369335	18.63432894258826
17	22.397421266336924	24.689500595003512	32.834694288864895	20.07838384979467
18	22.32283722321425	23.90810116215033	33.49439721073651	20.27466440389891
19	21.640540856265893	25.76037854290251	32.10104918310575	20.498031417725844
20	23.18742161294091	25.629310887087975	31.917220698163128	19.26604680180799
21	22.94441370651885	26.755003613667494	32.00823633770525	18.292346342108402
22	22.36738225413261	23.60463011577857	32.3240310813916	21.703956548697217
23	20.723837496646286	25.851009066903558	32.42043833850886	21.0047150979413
24	20.636801384362	24.85355981550398	33.09413379170641	21.415505008427612
25	21.155552018197994	26.324701182433085	31.555982320629226	20.963764478739698
26	21.174807795252036	25.825591441192213	31.84225153949938	21.15734922405637
27	22.381759900999633	25.31582683664811	30.821952099329003	21.48046116302326
28	20.475823088190175	26.131244808963437	32.06189576976253	21.331036333083865
29	22.035284285873832	24.317864097860426	31.002186172554868	22.64466544371087
30	22.528874037692542	24.82775707425156	30.376116674604326	22.26725221345157
31	22.18753329644782	25.7350892890382	28.726024952919627	23.351352461594352
32	22.856992478693485	26.001845987160248	28.70047895536126	22.44068257878501
33	21.11550000192558	26.74178131342372	28.20213944520255	23.940579239448155
34	21.33321865448332	27.219452956211075	28.594700553411034	22.852627835894566
35	20.900348786308374	25.963077689358105	28.875449782859018	24.261123741474506
36	20.96017006702294	29.02256391955193	28.086989898419358	21.93027611500577
37	20.5573392110523	27.45565715474071	28.78469088701095	23.20231274719604
38	19.97992264312498	28.416135314196517	29.155043665683767	22.448898376994737
39	21.32192193194495	27.27888912138457	28.17877576904364	23.220413177626842
40	21.55427497506377	25.981178119788908	30.62053667134368	21.844010233803644
41	20.39199627208156	26.779907751990727	29.54028757861171	23.287808397316002
42	21.754278312731792	26.2019776966753	29.87559484304616	22.16814914754675
43	21.627832043410226	26.090165817914805	29.59754142238574	22.68446071628923
44	21.90434500190632	26.062950986345086	29.152604600590255	22.88009941115834
45	21.349650250902773	24.758051161315915	29.478283976497682	24.41401461128363
46	22.44710117113636	25.235594432256253	28.81357455259202	23.50372984401537
47	20.804583388426252	25.43174661451346	30.65930496914583	23.104365027914458
48	20.852851202908393	24.958567986372046	30.089590712040128	24.098990098679437
49	21.083021924627754	24.482693549443056	30.62900921324746	23.805275312681726
50	20.675184566623063	24.40926485294363	30.801797719345764	24.11375286108754
51	20.636544640667946	23.844942213413063	30.007175986248807	25.511337159670187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	163.0
1	445.5
2	728.0
3	7473.5
4	14219.0
5	9590.5
6	4962.0
7	4900.0
8	4838.0
9	4755.5
10	4673.0
11	4676.5
12	4680.0
13	4479.5
14	4279.0
15	4032.5
16	3786.0
17	3766.0
18	3746.0
19	3617.5
20	3489.0
21	3790.5
22	4092.0
23	4354.0
24	4616.0
25	5135.0
26	6520.0
27	7386.0
28	8541.0
29	9696.0
30	11439.5
31	13183.0
32	15292.5
33	17402.0
34	19018.5
35	20635.0
36	23331.0
37	26027.0
38	28414.5
39	30802.0
40	33836.5
41	36871.0
42	39325.0
43	41779.0
44	45049.5
45	48320.0
46	59296.0
47	70272.0
48	72038.5
49	73805.0
50	72786.5
51	71768.0
52	62474.5
53	53181.0
54	47870.5
55	42560.0
56	38509.5
57	34459.0
58	31766.0
59	29073.0
60	26375.5
61	23678.0
62	20902.0
63	18126.0
64	16457.5
65	14789.0
66	12153.5
67	9518.0
68	8122.0
69	6726.0
70	5800.5
71	4875.0
72	4248.0
73	3621.0
74	3182.5
75	2256.5
76	1769.0
77	1378.0
78	987.0
79	777.5
80	568.0
81	402.0
82	236.0
83	185.0
84	134.0
85	89.5
86	45.0
87	34.5
88	24.0
89	13.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	778987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.54527291162681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18271969096645	25.657544709188613
2	8.21580787129447	5.8406626594930735
3	3.4917219194613582	3.7234262567629015
4	2.267362233869941	3.223760375696915
5	1.771947306197736	3.149217529191025
6	1.4146825316900136	3.0171166003339613
7	1.2701056451007502	3.1602376247192794
8	1.1001281560819667	3.128348443655863
9	0.9780815044563071	3.1289556605142583
>10	7.238182617892157	40.339364967044474
>50	0.045930665619677256	1.0948906298201193
>100	0.018590980091131196	1.3320087489074455
>500	0.0018226451069734784	0.5833369054067155
>1k	0.00255170314976287	1.5625188232562377
>5k	3.645290213946957E-4	1.0586100660090774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8170	1.048797990210363	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	2673	0.34313794710309675	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1864	0.23928512285827622	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1796	0.2305558372604421	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	1783	0.22888700324909145	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	1541	0.19782101626856416	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1230	0.15789737184317582	No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1172	0.15045180471561143	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	971	0.12464906346319002	Illumina PCR Primer Index 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	952	0.12220999836967754	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	891	0.11437931570103224	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	875	0.11232536614860068	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	813	0.10436631163292841	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.283718470269722E-4	0.0	0.0	0.1297839373442689	0.0
2	1.283718470269722E-4	0.0	0.0	0.6232453173159501	0.0
3	1.283718470269722E-4	0.0	0.0	1.0912890715762908	0.0
4	1.283718470269722E-4	0.0	0.0	1.5910406720522936	0.0
5	1.283718470269722E-4	0.0	0.0	2.7914458136015106	0.0
6	1.283718470269722E-4	0.0	0.0	3.99005374929235	0.0
7	1.283718470269722E-4	0.0	0.0	4.9736388412130115	0.0
8	1.283718470269722E-4	0.0	0.0	6.571098105616653	0.0
9	1.283718470269722E-4	0.0	0.0	7.419507642617913	0.0
10	1.283718470269722E-4	0.0	0.0	8.735960933879513	0.0
11	1.283718470269722E-4	0.0	0.0	9.759726413919616	0.0
12	1.283718470269722E-4	0.0	0.0	10.908397701116963	0.0
13	1.283718470269722E-4	0.0	0.0	11.387866549762705	0.0
14	1.283718470269722E-4	0.0	0.0	11.656677197437185	0.0
15	1.283718470269722E-4	0.0	0.0	11.977093327616506	0.0
16	1.283718470269722E-4	0.0	0.0	12.43486733411469	0.0
17	1.283718470269722E-4	0.0	0.0	12.986737904483643	0.0
18	1.283718470269722E-4	0.0	0.0	13.607672528553108	0.0
19	1.283718470269722E-4	0.0	0.0	14.06968280600318	0.0
20	1.283718470269722E-4	0.0	0.0	14.482911781583004	0.0
21	1.283718470269722E-4	0.0	0.0	14.93760486375254	0.0
22	1.283718470269722E-4	0.0	0.0	15.412323954058284	0.0
23	1.283718470269722E-4	0.0	0.0	15.864706342981334	0.0
24	1.283718470269722E-4	0.0	0.0	16.26676696786981	0.0
25	1.283718470269722E-4	0.0	0.0	16.622742099675605	0.0
26	1.283718470269722E-4	0.0	0.0	16.975893050846803	0.0
27	2.567436940539444E-4	0.0	0.0	17.34290816149692	0.0
28	2.567436940539444E-4	0.0	0.0	17.718652557744868	0.0
29	2.567436940539444E-4	0.0	0.0	18.110828550412265	0.0
30	2.567436940539444E-4	0.0	0.0	18.550117010938564	0.0
31	2.567436940539444E-4	0.0	0.0	18.95513018830866	0.0
32	2.567436940539444E-4	0.0	0.0	19.34486711588255	0.0
33	2.567436940539444E-4	0.0	0.0	19.73691473670292	0.0
34	3.851155410809166E-4	0.0	0.0	20.128577241982214	0.0
35	3.851155410809166E-4	0.0	0.0	20.523320671590156	0.0
36	3.851155410809166E-4	0.0	0.0	20.910490162223503	0.0
37	3.851155410809166E-4	0.0	0.0	21.302152667502796	0.0
38	3.851155410809166E-4	0.0	0.0	21.69843655927506	0.0
39	3.851155410809166E-4	0.0	0.0	22.117570639818123	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	20	7.031885E-4	45.0	2
CGTTTTT	5025	0.0	43.83582	1
ACACGAC	160	0.0	40.78125	26
CTCGATC	50	1.0804797E-9	40.500004	16
CACGACC	165	0.0	39.545452	27
CTTGCGG	110	0.0	38.86364	2
TAACGGG	245	0.0	38.57143	3
ACGTAGG	105	0.0	38.57143	2
AATAGCG	35	6.2456966E-6	38.571426	1
GCGAGAC	170	0.0	38.38235	21
TCAAGCG	170	0.0	38.38235	17
CTAACGG	65	9.094947E-12	38.076927	2
GTTTACG	30	1.1395842E-4	37.499996	1
TAGCGCG	30	1.1395842E-4	37.499996	1
TATGCGG	145	0.0	37.24138	2
TTAACGG	85	0.0	37.058823	2
CGTAAGG	110	0.0	36.818184	2
TACGGCT	335	0.0	36.268658	7
AACGTTG	25	0.0021066924	36.000004	1
TCCGAAT	25	0.0021066924	36.000004	16
>>END_MODULE