SRR3554408_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303687
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	4118	1.3560014093458066	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	3799	1.2509590466500047	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	3644	1.1999196541175619	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3442	1.1334038006236684	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	1474	0.48536815866336064	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGAGGTGT	1170	0.38526509201908543	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	743	0.24465979775229102	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	705	0.23214691442175664	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTA	640	0.21074329819847407	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	563	0.18538824513397018	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	545	0.17946108987213807	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	449	0.14784959514236698	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTT	349	0.1149209547988554	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	25	3.8840037E-5	45.000004	42
CATGCGG	25	3.8840037E-5	45.000004	2
ACACCCG	35	1.2079727E-7	45.000004	37
GACCCGT	25	3.8840037E-5	45.000004	38
CCGGTCG	25	3.8840037E-5	45.000004	40
GTCGAAT	75	0.0	45.0	43
TCCGACT	40	6.7866495E-9	45.0	11
CGCGAGG	20	7.02508E-4	45.0	2
TATTGCG	20	7.02508E-4	45.0	1
GCTTACT	20	7.02508E-4	45.0	37
CCGACCA	20	7.02508E-4	45.0	10
ACGTAAG	20	7.02508E-4	45.0	1
CCCGTCG	65	0.0	44.999996	40
CGTTTTT	1935	0.0	42.441864	1
ACCCGTC	85	0.0	42.352943	39
CGAACAC	80	0.0	42.1875	34
TCGAATG	80	0.0	42.1875	44
CGTCGAA	55	6.002665E-11	40.909092	42
ATTGCGG	55	6.002665E-11	40.909092	2
CGTAAGG	45	1.9203071E-8	40.0	2