FastQCFastQC Report
Sat 18 Jun 2016
SRR3554407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564192
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92641.641994214735409No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC44130.7821805342862004No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC34900.6185837445408655No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG32680.5792354375815325No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC20550.3642377063127446TruSeq Adapter, Index 27 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT11280.1999319380636379No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT9680.17157279791276728No Hit
CGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG8610.15260762293687255No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC7910.14020049912086666TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT7850.139137031365209TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT6710.11893114400771368No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC6070.10758748794736543No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA5910.10475157393227837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG207.0302276E-445.0000041
GTACGAG351.2104647E-745.0000041
TCACGAC453.8380676E-1045.025
GCGTAAC253.8882714E-545.032
CGAAAGG302.1635296E-644.9999962
TGCGACG302.1635296E-644.9999961
ACTACGG302.1635296E-644.9999962
CGTTTTT49850.043.3299871
CGTAAGG900.042.52
TAAGCGG650.041.538462
GCGTAAG603.6379788E-1241.2499961
CCGATGA5200.041.10576618
CGCCCAA451.9254003E-840.037
CGATGAA5600.039.77678719
TCGTTAG403.4540426E-739.3750041
TCAAGCG2650.039.05660217
CGAACCC356.2426334E-638.5714334
GCGAACC700.038.5714333
ACACGAC2700.038.33333226
AGTAAGG1900.037.8947372
Produced by FastQC (version 0.11.5)