FastQCFastQC Report
Sat 18 Jun 2016
SRR3554406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302023
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51081.6912619237607733No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC13310.4406949139635061Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC8800.29136853815769No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC8090.2678603947381491Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG7830.2592517788380355No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC7720.2556096721110644No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT5940.19667376325644073Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5780.19137615347175546No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC4230.14005555868261688Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG351.2079545E-745.0000042
TGTCACG207.02502E-445.015
CCTTATC207.02502E-445.043
GATCGTG253.883955E-545.029
TCCACCG253.883955E-545.031
TTAACGG207.02502E-445.02
TTACGGG253.883955E-545.03
CTTACGT207.02502E-445.022
GGCTACG253.883955E-545.01
TCGTGCG302.1601682E-644.9999961
CGAATAT302.1601682E-644.99999614
CGTTTTT26300.043.0323221
AGGGTAC603.6379788E-1241.2499966
CGTAAGG603.6379788E-1241.2499962
CTAGCGG603.6379788E-1241.2499962
CGGGTTC451.9203071E-840.0000046
AGGGCAT850.039.7058836
ATGGGCG750.039.0000045
GCCGATT356.233002E-638.571439
TACGAAT356.233002E-638.5714312
Produced by FastQC (version 0.11.5)