SRR3554403_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480502
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	12302	2.5602390832920574	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	8115	1.6888587352393953	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	7817	1.6268402628917256	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7682	1.5987446462241575	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	3902	0.8120673795322392	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	3587	0.746510940641246	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	2873	0.5979163458216615	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	1644	0.3421421763072786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	1519	0.3161277164299004	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	1347	0.2803318196386279	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	1230	0.25598228519340194	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1196	0.24890635210675505	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	949	0.1975017793890556	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	907	0.1887609208702565	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	859	0.17877136827734327	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	766	0.15941661012857386	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	685	0.14255924012803275	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	682	0.14193489309097568	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCGCCGATCGAATGC	669	0.13922938926372835	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	7.029183E-4	45.000004	29
TTGCACT	20	7.029183E-4	45.000004	27
CTAGGCG	20	7.029183E-4	45.000004	1
GACTACG	20	7.029183E-4	45.000004	1
GCGAACC	50	2.1827873E-11	45.0	33
TCGATAG	25	3.887406E-5	45.0	1
ACTACGG	25	3.887406E-5	45.0	2
GCGATAA	25	3.887406E-5	45.0	9
CGATCGA	330	0.0	43.636364	41
GATGAAT	2585	0.0	43.34623	20
CGTTTTT	3805	0.0	43.34428	1
ATGAATG	2560	0.0	43.24219	21
CGATGAA	2405	0.0	42.75468	19
TGAATGA	2580	0.0	42.73256	22
CACCCGT	90	0.0	42.5	38
ATCGAAT	335	0.0	42.313435	43
CCGATGA	2295	0.0	42.058823	18
CGCCGAT	115	0.0	41.086956	38
CCGATCG	340	0.0	41.02941	40
TCGAATG	330	0.0	40.90909	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content