##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480502
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73597820612609	31.0	31.0	34.0	30.0	34.0
2	31.82979675422787	33.0	31.0	34.0	30.0	34.0
3	31.8608496946943	33.0	31.0	34.0	30.0	34.0
4	35.57492580676043	37.0	35.0	37.0	33.0	37.0
5	35.5864741457892	37.0	35.0	37.0	33.0	37.0
6	35.53015804304665	37.0	35.0	37.0	33.0	37.0
7	35.771414062792665	37.0	35.0	37.0	35.0	37.0
8	35.42917407211625	37.0	35.0	37.0	33.0	37.0
9	37.14536880179479	39.0	37.0	39.0	34.0	39.0
10	36.9460501725279	39.0	37.0	39.0	33.0	39.0
11	36.93757153976466	39.0	37.0	39.0	33.0	39.0
12	36.90010239291408	39.0	37.0	39.0	33.0	39.0
13	36.787936366549985	39.0	35.0	39.0	32.0	39.0
14	37.888250204993945	40.0	37.0	41.0	33.0	41.0
15	37.91439369659231	40.0	37.0	41.0	32.0	41.0
16	38.072819676088756	40.0	37.0	41.0	33.0	41.0
17	37.92409396839139	40.0	37.0	41.0	33.0	41.0
18	37.8660047200636	39.0	37.0	41.0	33.0	41.0
19	37.660588301401454	39.0	37.0	41.0	33.0	41.0
20	37.14597233726394	39.0	35.0	41.0	31.0	41.0
21	37.330901848483464	39.0	35.0	41.0	32.0	41.0
22	37.28995508863647	39.0	35.0	41.0	32.0	41.0
23	37.004303832242115	39.0	35.0	41.0	31.0	41.0
24	37.08029519127912	39.0	35.0	41.0	31.0	41.0
25	37.148573783251685	39.0	35.0	41.0	32.0	41.0
26	37.02199366495873	39.0	35.0	41.0	32.0	41.0
27	36.78593429371782	39.0	35.0	41.0	31.0	41.0
28	36.69045914481105	39.0	35.0	41.0	31.0	41.0
29	36.697736950106346	39.0	35.0	41.0	31.0	41.0
30	36.48515094630199	39.0	35.0	41.0	30.0	41.0
31	36.34708284252719	39.0	35.0	41.0	30.0	41.0
32	35.879638378196134	39.0	35.0	41.0	27.0	41.0
33	35.5010405783951	39.0	35.0	41.0	25.0	41.0
34	34.89463311286946	38.0	34.0	41.0	22.0	41.0
35	34.689095987113475	38.0	34.0	41.0	20.0	41.0
36	34.54377088961128	38.0	34.0	41.0	19.0	41.0
37	34.400762119616566	38.0	34.0	41.0	18.0	41.0
38	34.3060715668197	38.0	34.0	40.0	18.0	41.0
39	33.91824383665416	38.0	33.0	40.0	15.0	41.0
40	34.02136723676488	38.0	33.0	40.0	17.0	41.0
41	33.90222517284007	38.0	33.0	40.0	18.0	41.0
42	33.92832496014585	38.0	33.0	40.0	17.0	41.0
43	33.8481213397655	38.0	34.0	40.0	15.0	41.0
44	33.973348706144826	38.0	34.0	40.0	16.0	41.0
45	33.97189189639169	38.0	33.0	40.0	18.0	41.0
46	33.792140303266166	37.0	33.0	40.0	17.0	41.0
47	33.528493117614495	37.0	33.0	40.0	15.0	41.0
48	33.51509046788567	37.0	33.0	40.0	15.0	41.0
49	33.566370171195956	37.0	33.0	40.0	17.0	41.0
50	33.48315719809699	37.0	33.0	40.0	15.0	41.0
51	32.69513342296182	35.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	6.0
11	11.0
12	12.0
13	11.0
14	26.0
15	36.0
16	61.0
17	109.0
18	225.0
19	443.0
20	1021.0
21	2281.0
22	4328.0
23	5923.0
24	7663.0
25	9245.0
26	10939.0
27	11041.0
28	9676.0
29	8859.0
30	9144.0
31	11016.0
32	13722.0
33	18001.0
34	29044.0
35	34523.0
36	37285.0
37	50903.0
38	83567.0
39	121262.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.163162692350916	14.816587652080532	17.478595302412895	17.541654353155657
2	35.13533762606607	32.78446291586716	18.159133572805107	13.921065885261664
3	25.650257439094947	33.625458374783044	27.42652475952233	13.297759426599681
4	23.230704554819752	19.559127745566094	41.194833736384034	16.015333963230123
5	31.67811996620201	22.518532701216646	27.101239953215593	18.702107379365746
6	21.385967175995106	37.79651281368236	28.458362296098667	12.359157714223874
7	63.65592651019143	4.456172919155383	27.40571319162043	4.482187379032761
8	61.35104536505571	14.929386350108844	17.366837182779676	6.352731102055767
9	56.20309592884109	5.483431910793295	18.669849449117798	19.643622711247822
10	38.411702760862596	19.401167945190657	25.769091491814812	16.418037802131938
11	32.19820104807056	19.70106263865707	29.63046980033382	18.47026651293855
12	22.047358803917568	17.750186263532722	34.11931688109519	26.08313805145452
13	27.368668600755043	18.36787359886119	38.44458503814761	15.818872762236161
14	19.007621196165676	29.566162055516937	32.67603464709825	18.750182101219142
15	16.779326620908964	20.532068544980042	39.31929523706457	23.369309597046424
16	19.73394491594208	21.95308240132195	30.699143812096512	27.613828870639455
17	19.904599772737676	27.167420739143644	32.36739909511302	20.560580393005647
18	20.788050830173443	20.98139029598212	31.830252527564923	26.400306346279518
19	19.44903455136503	23.221339349263896	29.653154409346893	27.676471690024187
20	25.87148440589217	23.04714652592497	33.33222338304523	17.749145685137627
21	20.85527219449659	30.23858381442741	31.59757919842165	17.308564792654348
22	19.014697129252323	19.294612717532914	37.847293039363	23.843397113851765
23	23.68606166051338	24.502707584984037	32.25147866189943	19.55975209260315
24	21.283366146238723	26.660659060732318	30.15991608775822	21.89605870527074
25	19.49398753803314	31.22588459569367	28.502899051408736	20.777228814864454
26	18.516051962322738	20.37202758781441	36.96446632896429	24.14745412089856
27	26.817162051354625	20.312922734973007	29.88874135799643	22.981173855675937
28	18.65444888887039	26.173876487506814	32.584463748329874	22.587210875292925
29	22.129356381451064	19.611156665320852	36.512855305493005	21.746631647735075
30	22.1133314741666	26.03194159441584	30.825053797903024	21.02967313351453
31	25.95223328935155	18.940816063200568	28.63505250758582	26.47189813986206
32	32.39382978634844	21.58929619439669	27.57678427977407	18.44008973948079
33	29.958043879109763	19.91895975458999	26.29666473812804	23.826331628172206
34	24.355986031275627	19.98326749940687	32.646274104998525	23.014472364318983
35	28.64733133264794	21.172232373642565	27.637554058047627	22.54288223566187
36	25.1520285035234	28.832762402653888	27.385734086434603	18.62947500738811
37	23.859630136815248	22.129564497130087	30.113714407015994	23.89709095903867
38	27.02069918543523	22.59366246134251	25.179499773153907	25.206138580068345
39	26.357434516401597	24.34599647868271	28.32412768313139	20.9724413217843
40	26.035895792317206	20.639456235353858	29.82464172885857	23.50000624347037
41	20.748092619801792	25.05525471277955	28.530994668076303	25.665657999342358
42	25.467115641558202	21.81843155699664	30.534316194313448	22.180136607131708
43	24.430075213006397	24.60239499523415	27.652538386936993	23.314991404822457
44	23.63444897211666	20.726032357825773	30.75075650049323	24.88876216956433
45	23.365979746182116	19.16891084740542	29.159087787355727	28.306021619056736
46	28.382816304614757	22.602611435540332	27.857948562128776	21.15662369771614
47	20.382225256086343	23.90603993323649	34.78736821074626	20.924366599930906
48	22.050480539102853	23.332056890502017	29.82651476996974	24.79094780042539
49	23.38928870223225	19.71438204211429	34.544913444689094	22.35141581096437
50	22.881278329746806	21.431752625379293	32.00590216065698	23.68106688421692
51	21.493979213405982	19.741645196065782	31.338475178042962	27.425900412485277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	421.0
2	656.0
3	6525.5
4	12395.0
5	8128.0
6	3861.0
7	3680.0
8	3499.0
9	3433.5
10	3368.0
11	3318.5
12	3269.0
13	3087.0
14	2905.0
15	2802.5
16	2700.0
17	2541.0
18	2382.0
19	2306.0
20	2230.0
21	2140.5
22	2051.0
23	2083.0
24	2115.0
25	2383.0
26	2869.5
27	3088.0
28	3555.5
29	4023.0
30	4398.5
31	4774.0
32	5469.5
33	6165.0
34	6928.0
35	7691.0
36	8623.0
37	9555.0
38	10407.5
39	11260.0
40	11971.5
41	12683.0
42	13739.5
43	14796.0
44	16487.5
45	18179.0
46	21345.5
47	24512.0
48	48027.5
49	71543.0
50	58683.5
51	45824.0
52	46670.5
53	47517.0
54	41344.5
55	35172.0
56	32289.0
57	29406.0
58	25683.5
59	21961.0
60	19730.5
61	17500.0
62	15892.5
63	14285.0
64	12957.5
65	11630.0
66	9765.5
67	7901.0
68	6832.5
69	5764.0
70	4845.0
71	3926.0
72	3396.5
73	2867.0
74	2370.5
75	1480.0
76	1086.0
77	876.0
78	666.0
79	495.5
80	325.0
81	243.0
82	161.0
83	113.5
84	66.0
85	43.0
86	20.0
87	14.5
88	9.0
89	4.5
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.93229052726301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14208200510146	29.938729504017086
2	8.750380233278063	7.163462118651143
3	4.38326686557576	5.382514584008176
4	3.0505549032521775	4.994647982771392
5	2.2612332193488704	4.62787275421431
6	1.8205967838457553	4.471271789362506
7	1.4522732411660264	4.1611409162664375
8	1.1734627051409277	3.8426013095789138
9	0.9402447089491903	3.4637732634087093
>10	2.944416037095436	16.378046126679177
>50	0.04612601730923696	1.3119804786343938
>100	0.02562556517178736	1.9432163692974842
>500	0.0035875791240502306	1.1573706908576293
>1k	0.004100090427485978	3.628819686506303
>5k	0.001537533910307242	4.953806687314131
>10k+	5.125113034357472E-4	2.5807457384322197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	12302	2.5602390832920574	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	8115	1.6888587352393953	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	7817	1.6268402628917256	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7682	1.5987446462241575	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	3902	0.8120673795322392	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	3587	0.746510940641246	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	2873	0.5979163458216615	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	1644	0.3421421763072786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	1519	0.3161277164299004	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	1347	0.2803318196386279	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	1230	0.25598228519340194	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1196	0.24890635210675505	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	949	0.1975017793890556	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	907	0.1887609208702565	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	859	0.17877136827734327	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	766	0.15941661012857386	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	685	0.14255924012803275	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	682	0.14193489309097568	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCGCCGATCGAATGC	669	0.13922938926372835	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1483864791405655	0.0
2	0.0	0.0	0.0	1.195416460285285	0.0
3	0.0	0.0	0.0	1.7275682515369344	0.0
4	0.0	0.0	0.0	2.879904766265281	0.0
5	0.0	0.0	0.0	6.3602232665004514	0.0
6	0.0	0.0	0.0	7.658865103579173	0.0
7	0.0	0.0	0.0	8.782481654602895	0.0
8	0.0	0.0	0.0	10.29194467452789	0.0
9	0.0	0.0	0.0	11.006822031958244	0.0
10	0.0	0.0	0.0	13.630744513030123	0.0
11	0.0	0.0	0.0	14.574757233060424	0.0
12	0.0	0.0	0.0	17.09961665091925	0.0
13	0.0	0.0	0.0	17.618449038713678	0.0
14	0.0	0.0	0.0	17.917719385143037	0.0
15	0.0	0.0	0.0	18.439257276764717	0.0
16	0.0	0.0	0.0	18.91063928974281	0.0
17	2.0811567901902594E-4	0.0	0.0	19.38951346716559	0.0
18	2.0811567901902594E-4	0.0	0.0	19.907513392243946	0.0
19	2.0811567901902594E-4	0.0	0.0	20.85069364955817	0.0
20	2.0811567901902594E-4	0.0	0.0	21.25006763759568	0.0
21	4.162313580380519E-4	0.0	0.0	21.579514757482798	0.0
22	4.162313580380519E-4	0.0	0.0	22.01718203045981	0.0
23	4.162313580380519E-4	0.0	0.0	22.344964224914776	0.0
24	4.162313580380519E-4	0.0	0.0	22.65568093369018	0.0
25	4.162313580380519E-4	0.0	0.0	22.916241763822004	0.0
26	4.162313580380519E-4	0.0	0.0	23.19428431099142	0.0
27	4.162313580380519E-4	0.0	0.0	23.580547011250733	0.0
28	4.162313580380519E-4	0.0	0.0	23.846102617679012	0.0
29	4.162313580380519E-4	0.0	0.0	24.156819326454418	0.0
30	4.162313580380519E-4	0.0	0.0	24.554736504738795	0.0
31	4.162313580380519E-4	0.0	0.0	24.86732625462537	0.0
32	4.162313580380519E-4	0.0	0.0	25.189073094388785	0.0
33	4.162313580380519E-4	0.0	0.0	25.475648384397985	0.0
34	6.243470370570778E-4	0.0	0.0	25.778456697370668	0.0
35	6.243470370570778E-4	0.0	0.0	26.095416876516644	0.0
36	6.243470370570778E-4	0.0	0.0	26.370753919858814	0.0
37	6.243470370570778E-4	0.0	0.0	26.671689191720326	0.0
38	6.243470370570778E-4	0.0	0.0	27.007587897657032	0.0
39	6.243470370570778E-4	0.0	0.0	27.484589033968643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	7.029183E-4	45.000004	29
TTGCACT	20	7.029183E-4	45.000004	27
CTAGGCG	20	7.029183E-4	45.000004	1
GACTACG	20	7.029183E-4	45.000004	1
GCGAACC	50	2.1827873E-11	45.0	33
TCGATAG	25	3.887406E-5	45.0	1
ACTACGG	25	3.887406E-5	45.0	2
GCGATAA	25	3.887406E-5	45.0	9
CGATCGA	330	0.0	43.636364	41
GATGAAT	2585	0.0	43.34623	20
CGTTTTT	3805	0.0	43.34428	1
ATGAATG	2560	0.0	43.24219	21
CGATGAA	2405	0.0	42.75468	19
TGAATGA	2580	0.0	42.73256	22
CACCCGT	90	0.0	42.5	38
ATCGAAT	335	0.0	42.313435	43
CCGATGA	2295	0.0	42.058823	18
CGCCGAT	115	0.0	41.086956	38
CCGATCG	340	0.0	41.02941	40
TCGAATG	330	0.0	40.90909	44
>>END_MODULE