FastQCFastQC Report
Sat 18 Jun 2016
SRR3554402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434318
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.7162954333000243No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG10150.2336997315331163No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC9360.21551029430048949Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC5910.1360754101833219Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT4460.10268973425001957Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTG207.028435E-445.01
CCGATTA253.8867838E-545.011
GGTCCGA253.8867838E-545.08
GTAGCCG207.028435E-445.043
TGATTCG551.8189894E-1245.015
ACTCGGC207.028435E-445.038
TTCGGCA603.6379788E-1241.25000418
CGTTTTT17650.041.175641
GTTGTAG556.002665E-1140.9090921
AAGGGCG1900.040.2631575
CCGATGT451.9235813E-840.011
CGACGGT403.4516052E-739.37528
CGTTCGG403.4516052E-739.37545
GTACGAG403.4516052E-739.3751
CATACGG356.2393156E-638.571432
CGATGTA356.2393156E-638.5714310
ACAACGA1350.038.33333213
TAGGGAT2300.038.1521765
CGTGATG301.1387524E-437.50000434
TAACGAG301.1387524E-437.5000041