##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554402_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 434318 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01405652079813 33.0 31.0 34.0 30.0 34.0 2 32.07381918317915 33.0 31.0 34.0 30.0 34.0 3 32.12072260417482 34.0 31.0 34.0 30.0 34.0 4 35.70317831634885 37.0 35.0 37.0 33.0 37.0 5 35.795435602484815 37.0 35.0 37.0 35.0 37.0 6 35.774374997121924 37.0 35.0 37.0 35.0 37.0 7 36.02683057114833 37.0 35.0 37.0 35.0 37.0 8 35.893379965831485 37.0 35.0 37.0 35.0 37.0 9 37.646081903121676 39.0 38.0 39.0 35.0 39.0 10 37.24057027339415 39.0 37.0 39.0 34.0 39.0 11 37.23106801928541 39.0 37.0 39.0 34.0 39.0 12 37.23605745099213 39.0 37.0 39.0 34.0 39.0 13 37.24608236361376 39.0 37.0 39.0 34.0 39.0 14 38.424942093120706 40.0 38.0 41.0 34.0 41.0 15 38.57847475812653 40.0 38.0 41.0 34.0 41.0 16 38.5738329979416 40.0 38.0 41.0 34.0 41.0 17 38.52250885296027 40.0 38.0 41.0 34.0 41.0 18 38.43710829392288 40.0 38.0 41.0 34.0 41.0 19 38.34136047780658 40.0 37.0 41.0 34.0 41.0 20 38.26291565166537 40.0 37.0 41.0 34.0 41.0 21 38.16733361269853 40.0 37.0 41.0 34.0 41.0 22 38.20631887234699 40.0 37.0 41.0 34.0 41.0 23 38.12509037157106 40.0 37.0 41.0 34.0 41.0 24 38.067630169599234 40.0 36.0 41.0 34.0 41.0 25 37.89876311826818 40.0 36.0 41.0 33.0 41.0 26 37.92269028684052 40.0 36.0 41.0 34.0 41.0 27 37.88680413890283 40.0 36.0 41.0 34.0 41.0 28 37.82735230867705 40.0 36.0 41.0 33.0 41.0 29 37.78079886166358 40.0 36.0 41.0 33.0 41.0 30 37.60252165464015 40.0 36.0 41.0 33.0 41.0 31 37.589227248237464 40.0 36.0 41.0 33.0 41.0 32 37.444255131033024 40.0 36.0 41.0 33.0 41.0 33 37.31499500366091 40.0 35.0 41.0 33.0 41.0 34 37.156134445268215 40.0 35.0 41.0 32.0 41.0 35 36.9918608024535 40.0 35.0 41.0 31.0 41.0 36 36.85885226953523 40.0 35.0 41.0 31.0 41.0 37 36.82381342702812 40.0 35.0 41.0 31.0 41.0 38 36.70195801233198 40.0 35.0 41.0 31.0 41.0 39 36.638159136853645 39.0 35.0 41.0 31.0 41.0 40 36.530146114137565 39.0 35.0 41.0 30.0 41.0 41 36.466513015808694 39.0 35.0 41.0 30.0 41.0 42 36.459799041255486 39.0 35.0 41.0 30.0 41.0 43 36.35621134744588 39.0 35.0 41.0 30.0 41.0 44 36.25363673621632 39.0 35.0 41.0 30.0 41.0 45 36.211697419862865 39.0 35.0 41.0 30.0 41.0 46 36.09265791424716 39.0 35.0 41.0 30.0 41.0 47 36.01137645688183 39.0 35.0 41.0 29.0 41.0 48 35.88636206650427 39.0 35.0 41.0 29.0 41.0 49 35.84913588660843 38.0 35.0 41.0 29.0 41.0 50 35.80437145133289 38.0 35.0 41.0 29.0 41.0 51 35.02666249153846 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 6.0 10 14.0 11 14.0 12 18.0 13 20.0 14 38.0 15 36.0 16 48.0 17 97.0 18 145.0 19 293.0 20 440.0 21 622.0 22 966.0 23 1424.0 24 2147.0 25 3204.0 26 4638.0 27 5464.0 28 5724.0 29 5850.0 30 6330.0 31 7666.0 32 9618.0 33 13072.0 34 23297.0 35 30884.0 36 31747.0 37 46390.0 38 80877.0 39 153100.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7031898286509 18.774031930520955 22.363337462412332 14.159440778415815 2 36.98764499744427 22.324886373578806 23.392767511362642 17.294701117614284 3 33.553525297132516 22.762814343407364 27.364281471180103 16.319378888280017 4 28.399007179071557 25.306572603484085 27.466971205430124 18.827449012014238 5 24.870947094064718 29.497280794256742 26.560722788371656 19.071049323306884 6 25.7999898691742 34.62693233989842 25.884259920150672 13.688817870776711 7 78.43607679165957 5.989850754516276 11.221040804203373 4.353031649620784 8 78.46623902302 5.362890785092951 11.047435289350199 5.123434902536851 9 72.26271994253058 7.2732421866006005 13.714375181318758 6.749662689550052 10 36.60037115661796 25.727922858366448 21.056921426236073 16.61478455877951 11 28.31220442164497 25.4854737772784 27.35092720080678 18.85139460026985 12 24.9720251060283 24.031700274913774 31.582158694781242 19.414115924276683 13 24.704018714398206 23.753102565401388 32.95810903531514 18.58476968488527 14 22.202855971891562 26.023098282825025 31.94963137608849 19.82441436919492 15 21.610663154647057 25.199047702374756 33.768114607269325 19.42217453570886 16 24.465023323923944 23.897927325139644 31.652383737261637 19.984665613674775 17 24.49334358695702 23.418555067945608 30.200452203224366 21.887649141873005 18 25.032119322708247 22.927670508705603 31.19695706832321 20.84325310026294 19 24.338388001418316 24.434861092563516 30.005894298647533 21.220856607370635 20 25.118691834093916 24.78828876537468 29.704732477125056 20.38828692340635 21 25.48593426936024 24.913312365593875 30.076810079250688 19.5239432857952 22 23.952495636837526 23.276263014657463 30.31856842221598 22.452672926289033 23 22.602332852886594 25.223914274794048 30.234758863321343 21.938994008998016 24 21.847356084712125 25.30035596037926 30.820274545379196 22.032013409529423 25 23.097822332944983 24.95844058961406 29.561979931755072 22.381757145685878 26 21.883504713136457 25.262135117586652 30.125622239925587 22.728737929351304 27 22.777319843985282 24.800031313461567 30.552958891871857 21.8696899506813 28 22.058721950276063 25.585630805078306 30.425402585202548 21.930244659443083 29 22.373007796130945 25.21539517128003 29.581320599192296 22.83027643339673 30 23.019768925073333 25.38646797968309 29.95247721715425 21.641285878089324 31 23.820794901431668 25.620397957257126 28.413742925690393 22.145064215620813 32 22.978785129789696 26.761267090012385 27.96430265381587 22.295645126382052 33 22.76811000234851 26.608383718841957 27.850330863560803 22.773175415248733 34 22.956221017779598 26.734328303224824 28.36700297938377 21.942447699611805 35 22.186968995068128 26.567630169599234 28.19961410763542 23.04578672769722 36 23.741129771273584 28.368154209588365 26.526876620356514 21.363839398781536 37 23.02138064735977 27.841811760046788 27.206562933150362 21.930244659443083 38 22.298638324914002 28.789964956552573 26.715217881828522 22.196178836704902 39 22.978324637707857 27.017761179596516 27.24593500614757 22.75797917654806 40 22.858366450388885 26.376986447718032 28.904166992848555 21.860480109044524 41 21.538365897798386 27.00256494089584 28.105213230858496 23.353855930447274 42 22.7918253445632 26.884218475863307 27.56597700302543 22.75797917654806 43 22.988685709549227 26.096546769878294 27.913878770854534 23.000888749717948 44 22.249595918198185 26.068686998927053 28.251879958924107 23.42983712395065 45 22.171542510326535 25.676117499159602 28.298619905230733 23.853720085283133 46 23.509502254108742 26.28880221404593 27.998379067871927 22.2033164639734 47 22.05365653737584 27.291753968290518 28.682900547525087 21.97168894680856 48 21.861631339249122 26.76794422519905 28.504690111853527 22.8657343236983 49 22.705943571300292 25.87159638790011 28.65135683991914 22.77110320088046 50 21.86508502986291 25.845578585276225 28.748981161268933 23.54035522359193 51 22.207691138750867 25.039026703935825 28.1588605583927 24.594421598920608 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 158.0 1 503.0 2 848.0 3 3195.0 4 5542.0 5 3861.5 6 2181.0 7 2102.5 8 2024.0 9 1991.0 10 1958.0 11 1938.0 12 1918.0 13 1955.5 14 1993.0 15 2009.0 16 2025.0 17 1858.0 18 1691.0 19 1691.0 20 1691.0 21 1758.5 22 1826.0 23 2065.5 24 2305.0 25 2699.5 26 3511.5 27 3929.0 28 4546.5 29 5164.0 30 5998.0 31 6832.0 32 7515.5 33 8199.0 34 9407.5 35 10616.0 36 11987.0 37 13358.0 38 14287.0 39 15216.0 40 16808.5 41 18401.0 42 19783.5 43 21166.0 44 23407.5 45 25649.0 46 29106.0 47 32563.0 48 35260.5 49 37958.0 50 37665.0 51 37372.0 52 34708.0 53 32044.0 54 29116.5 55 26189.0 56 24088.0 57 21987.0 58 20267.0 59 18547.0 60 17900.0 61 17253.0 62 15894.5 63 14536.0 64 13596.5 65 12657.0 66 10663.5 67 8670.0 68 7136.0 69 5602.0 70 4916.0 71 4230.0 72 3469.0 73 2708.0 74 2222.5 75 1450.5 76 1164.0 77 931.0 78 698.0 79 540.5 80 383.0 81 263.0 82 143.0 83 105.0 84 67.0 85 40.5 86 14.0 87 9.5 88 5.0 89 4.0 90 3.0 91 2.0 92 1.0 93 2.0 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 434318.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.50594057104384 #Duplication Level Percentage of deduplicated Percentage of total 1 72.25880038615138 24.93357871859459 2 7.77199918832101 5.363602842208114 3 3.3232688916008772 3.4401755662553573 4 2.1819048490546993 3.011547162126153 5 1.7324157127639204 2.9889316814487197 6 1.4266595407795977 2.9536937597552093 7 1.2649023386246663 3.055265144732997 8 1.1098093381070115 3.0636012052728026 9 0.9546232819909422 2.9646156812502844 >10 7.916949487597316 44.772175526447754 >50 0.035065324372923395 0.8344084092527808 >100 0.020904327991550488 1.3030359988331213 >500 0.0013486663220355153 0.3553099946818172 >1k 0.0013486663220355153 0.9600583091402604 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3111 0.7162954333000243 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 1015 0.2336997315331163 No Hit GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC 936 0.21551029430048949 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC 591 0.1360754101833219 Illumina Single End Adapter 1 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT 446 0.10268973425001957 Illumina Single End Adapter 1 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1091366233957607 0.0 2 2.302460409193264E-4 0.0 0.0 0.5903508489171528 0.0 3 2.302460409193264E-4 0.0 0.0 0.8364838666599128 0.0 4 2.302460409193264E-4 0.0 0.0 1.0775514715024475 0.0 5 2.302460409193264E-4 0.0 0.0 1.7639149194829595 0.0 6 2.302460409193264E-4 0.0 0.0 2.273449408037429 0.0 7 2.302460409193264E-4 0.0 0.0 2.7065422110066817 0.0 8 2.302460409193264E-4 0.0 0.0 3.513554584428921 0.0 9 2.302460409193264E-4 0.0 0.0 3.9061240841963722 0.0 10 2.302460409193264E-4 0.0 0.0 4.633241081419605 0.0 11 2.302460409193264E-4 0.0 0.0 5.4420954231691985 0.0 12 2.302460409193264E-4 0.0 0.0 6.162074793123932 0.0 13 2.302460409193264E-4 0.0 0.0 6.454487265091477 0.0 14 2.302460409193264E-4 0.0 0.0 6.5723732380421716 0.0 15 2.302460409193264E-4 0.0 0.0 6.789955746710936 0.0 16 2.302460409193264E-4 0.0 0.0 7.276005139091633 0.0 17 2.302460409193264E-4 0.0 0.0 7.819385795661244 0.0 18 2.302460409193264E-4 0.0 0.0 8.427235343688265 0.0 19 2.302460409193264E-4 0.0 0.0 8.758329150530257 0.0 20 2.302460409193264E-4 0.0 0.0 9.090113695495006 0.0 21 2.302460409193264E-4 0.0 0.0 9.486136885876247 0.0 22 2.302460409193264E-4 0.0 0.0 9.866963837556813 0.0 23 2.302460409193264E-4 0.0 0.0 10.272887607697585 0.0 24 2.302460409193264E-4 0.0 0.0 10.603290676416819 0.0 25 2.302460409193264E-4 0.0 0.0 10.896624132548041 0.0 26 2.302460409193264E-4 0.0 0.0 11.179826762878813 0.0 27 2.302460409193264E-4 0.0 0.0 11.47776513982842 0.0 28 2.302460409193264E-4 0.0 0.0 11.7646517068139 0.0 29 2.302460409193264E-4 0.0 0.0 12.071799925400283 0.0 30 2.302460409193264E-4 0.0 0.0 12.433286209643626 0.0 31 4.604920818386528E-4 0.0 0.0 12.753788698603328 0.0 32 4.604920818386528E-4 0.0 0.0 13.07452143360395 0.0 33 4.604920818386528E-4 0.0 0.0 13.377755469494701 0.0 34 4.604920818386528E-4 0.0 0.0 13.68283147371281 0.0 35 4.604920818386528E-4 0.0 0.0 14.008859867654575 0.0 36 4.604920818386528E-4 0.0 0.0 14.315777840200038 0.0 37 4.604920818386528E-4 0.0 0.0 14.614867447354243 0.0 38 4.604920818386528E-4 0.0 0.0 14.929844031331882 0.0 39 4.604920818386528E-4 0.0 0.0 15.279587767488339 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTG 20 7.028435E-4 45.0 1 CCGATTA 25 3.8867838E-5 45.0 11 GGTCCGA 25 3.8867838E-5 45.0 8 GTAGCCG 20 7.028435E-4 45.0 43 TGATTCG 55 1.8189894E-12 45.0 15 ACTCGGC 20 7.028435E-4 45.0 38 TTCGGCA 60 3.6379788E-12 41.250004 18 CGTTTTT 1765 0.0 41.17564 1 GTTGTAG 55 6.002665E-11 40.909092 1 AAGGGCG 190 0.0 40.263157 5 CCGATGT 45 1.9235813E-8 40.0 11 CGACGGT 40 3.4516052E-7 39.375 28 CGTTCGG 40 3.4516052E-7 39.375 45 GTACGAG 40 3.4516052E-7 39.375 1 CATACGG 35 6.2393156E-6 38.57143 2 CGATGTA 35 6.2393156E-6 38.57143 10 ACAACGA 135 0.0 38.333332 13 TAGGGAT 230 0.0 38.152176 5 CGTGATG 30 1.1387524E-4 37.500004 34 TAACGAG 30 1.1387524E-4 37.500004 1 >>END_MODULE