FastQCFastQC Report
Sat 18 Jun 2016
SRR3554399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664562
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128471.9331529639070546No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG20240.304561500657576No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC19600.2949310974747277No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT15840.2383524787754942No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC15080.22691637499586192No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC12810.19275853870669704No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC10900.1640178042078843No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG10070.15152837508012798No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG10070.15152837508012798No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC9440.14204844694701171No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT8690.13076281821711142No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT8350.12564666652622328Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT8190.12323906573051123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69250.043.4404341
AGTACGG603.6379788E-1241.2499962
ACGGGCT1550.039.193555
CCGATGA3050.037.6229518
CGTTCTG1500.037.51
ACGTTAG301.13940376E-437.4999961
AACGATA301.13940376E-437.49999615
TGAATCG301.13940376E-437.49999619
ATACGAG301.13940376E-437.4999961
GTTTTTT83250.037.270272
GCGCGAC2000.037.1259
CGTTTCT2750.036.8181841
ATTACGG250.002106470536.02
GGATCGA250.002106470536.08
TAGCACG250.002106470536.01
CTACGGG1000.036.03
ATCGGGT250.002106470536.04
CGATCAT250.002106470536.010
ATCGCGC250.002106470536.022
AGTCGAT250.002106470536.043
Produced by FastQC (version 0.11.5)