##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664562
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941743584496255	33.0	31.0	34.0	30.0	34.0
2	32.02834799461901	33.0	31.0	34.0	30.0	34.0
3	32.03888425760125	33.0	31.0	34.0	30.0	34.0
4	35.66915201290474	37.0	35.0	37.0	33.0	37.0
5	35.756854890890544	37.0	35.0	37.0	35.0	37.0
6	35.72673430018568	37.0	35.0	37.0	35.0	37.0
7	35.98155627315435	37.0	35.0	37.0	35.0	37.0
8	35.84285890556487	37.0	35.0	37.0	35.0	37.0
9	37.59762520276513	39.0	38.0	39.0	35.0	39.0
10	37.23912893003211	39.0	37.0	39.0	34.0	39.0
11	37.23463724979761	39.0	37.0	39.0	34.0	39.0
12	37.193607217987186	39.0	37.0	39.0	34.0	39.0
13	37.16654578504338	39.0	37.0	39.0	34.0	39.0
14	38.24684980483386	40.0	38.0	41.0	33.0	41.0
15	38.40537978397802	40.0	38.0	41.0	34.0	41.0
16	38.4314420625916	40.0	38.0	41.0	34.0	41.0
17	38.383035743843315	40.0	38.0	41.0	34.0	41.0
18	38.2135286098212	40.0	37.0	41.0	34.0	41.0
19	37.99345584008716	40.0	37.0	41.0	34.0	41.0
20	37.80327644373286	40.0	35.0	41.0	34.0	41.0
21	37.70388917813538	40.0	35.0	41.0	33.0	41.0
22	37.77689365326335	40.0	35.0	41.0	34.0	41.0
23	37.68692462102859	39.0	35.0	41.0	33.0	41.0
24	37.63326672304465	39.0	35.0	41.0	33.0	41.0
25	37.46671190949829	39.0	35.0	41.0	33.0	41.0
26	37.49484322004568	39.0	35.0	41.0	33.0	41.0
27	37.43642579623872	39.0	35.0	41.0	33.0	41.0
28	37.34282279155293	39.0	35.0	41.0	33.0	41.0
29	37.26516261838624	39.0	35.0	41.0	33.0	41.0
30	37.01238861084443	39.0	35.0	41.0	32.0	41.0
31	36.916329853347015	39.0	35.0	41.0	32.0	41.0
32	36.61119052849847	39.0	35.0	41.0	31.0	41.0
33	36.28817025349027	39.0	35.0	41.0	30.0	41.0
34	35.958756293618954	39.0	35.0	41.0	28.0	41.0
35	35.71090883920537	39.0	35.0	41.0	26.0	41.0
36	35.521686464167374	39.0	35.0	41.0	25.0	41.0
37	35.4568768602478	39.0	35.0	41.0	24.0	41.0
38	35.331323488252416	39.0	35.0	41.0	24.0	41.0
39	35.22989879048155	39.0	35.0	41.0	23.0	41.0
40	35.10824121752372	38.0	35.0	41.0	23.0	41.0
41	35.011715987372135	38.0	35.0	41.0	23.0	41.0
42	34.98072414612933	38.0	35.0	41.0	23.0	41.0
43	34.88789157369816	38.0	35.0	41.0	22.0	41.0
44	34.80222462313524	38.0	35.0	40.0	22.0	41.0
45	34.77228610724056	38.0	35.0	40.0	23.0	41.0
46	34.676936087227375	38.0	34.0	40.0	22.0	41.0
47	34.59390846903675	38.0	34.0	40.0	22.0	41.0
48	34.46353688594894	37.0	34.0	40.0	22.0	41.0
49	34.42281683274096	37.0	34.0	40.0	22.0	41.0
50	34.35926670498765	37.0	34.0	40.0	21.0	41.0
51	33.55713086213175	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	7.0
10	13.0
11	23.0
12	20.0
13	34.0
14	42.0
15	57.0
16	78.0
17	149.0
18	267.0
19	483.0
20	837.0
21	1333.0
22	2065.0
23	3256.0
24	5045.0
25	8400.0
26	12220.0
27	14204.0
28	13673.0
29	12597.0
30	12748.0
31	14021.0
32	17063.0
33	22961.0
34	40910.0
35	48611.0
36	50844.0
37	68881.0
38	117340.0
39	196206.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.204224737496276	17.662008962293964	21.095849597178294	21.03791670303147
2	40.27870988711362	22.15444157204294	21.57255455472928	15.994293986114164
3	29.506502026898918	22.671925268071302	32.21099009573223	15.610582609297552
4	26.019694174508924	23.35583436910326	33.10661759173711	17.5178538646507
5	23.972330647855276	26.948727131554318	31.12772021271153	17.951222007878872
6	24.03553016874272	31.46418242391229	30.972730911487567	13.527556495857421
7	71.99478754427729	5.485718413029936	17.807819285484275	4.711674757208508
8	71.93504894953368	5.410330413114202	17.28552038786449	5.369100249487632
9	65.94328294425502	6.521438180335319	19.749248377126587	7.786030498283079
10	34.680135186784675	22.871154233916474	26.80487298401052	15.643837595288327
11	28.465365157803184	22.627836078499826	31.85692230371282	17.04987645998417
12	24.529840707112356	20.867277996635377	34.96016925433594	19.64271204191633
13	23.657386368766193	20.6492396495737	37.64344034115703	18.04993364050307
14	20.06750310730978	23.429266193372477	36.7682774519157	19.73495324740205
15	19.97255335092888	21.814067009549145	38.83324655938799	19.380133080133984
16	23.506610368934727	22.460507823197837	35.26713835578923	18.76574345207821
17	22.937814680947753	22.51121791495752	34.19816360249307	20.352803801601656
18	24.50696849955309	20.940107920705668	34.4625482648722	20.090375314869043
19	22.990781898453417	24.132586575819865	32.68137510119447	20.19525642453225
20	23.595691598376074	24.54007301049413	32.48003948465305	19.384195906476748
21	22.53213394687027	25.27288650268899	33.643512569180906	18.551466981259836
22	21.925117596251365	21.988016167039344	34.110587123549045	21.976279113160246
23	20.649691074722902	24.735239149996538	34.02586967054992	20.589200104730633
24	20.75246553368986	23.683117602270368	34.31598556643323	21.248431297606544
25	22.45027551981606	24.291036803187662	32.32128830718579	20.937399369810493
26	20.494852248548668	23.022381658897135	33.759378357474546	22.723387735079648
27	22.007728398554235	21.4268947065887	33.64306114403171	22.922315750825355
28	19.9895570315486	23.921018655896667	33.025511539931564	23.063912772623173
29	21.691429844017563	23.139150297489174	32.32459875827989	22.844821100213373
30	22.189201308531032	22.5097131644602	33.2664220945525	22.034663432456263
31	22.20876306499619	22.67042051757398	31.696816850797973	23.423999566631856
32	22.79426148350342	22.3362154321192	31.771602950514776	23.097920133862605
33	22.43552896494232	23.13042274460472	29.577977675521623	24.85607061493134
34	21.060939385640467	23.623679957626226	32.584619644216794	22.730761012516513
35	21.473993397154818	24.296002479828818	31.096270927317544	23.13373319569882
36	22.9813019703203	26.20101660943599	28.394641884429145	22.423039535814567
37	22.759200796915863	25.331270821985008	29.128207751872665	22.781320629226467
38	23.573270815966005	25.550813919544	28.4409881997466	22.434927064743395
39	24.081876484060178	23.85601343441244	29.078701460510832	22.983408621016547
40	24.99360481038639	22.821798417604377	31.342899533828295	20.841697238180938
41	23.0870859302819	23.886409394458305	30.031058050264686	22.995446624995107
42	23.81478327078587	23.453342201329598	30.361651734525896	22.370222793358632
43	22.905763495354837	22.768680725048977	30.823459662153418	23.50209611744277
44	22.730761012516513	23.416475814145258	30.11321742741836	23.73954574591987
45	21.768322594430618	22.50670366346556	30.831735789888683	24.893237952215145
46	23.037579638920068	23.50375134298982	29.81903870519229	23.63963031289782
47	21.31328604404104	23.86398861204824	31.750235493452834	23.072489850457895
48	21.88644550847024	23.868051438391	30.641836277126888	23.60366677601187
49	22.728503886770536	22.624074202256526	31.091154775626656	23.55626713534629
50	21.09043249538794	22.518741667444118	32.197898766405544	24.192927070762398
51	21.234738068080933	22.143456893412502	31.097625202765126	25.524179835741435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	696.5
2	1127.0
3	10818.0
4	20509.0
5	13447.0
6	6385.0
7	6254.5
8	6124.0
9	5863.5
10	5603.0
11	5497.5
12	5392.0
13	5190.0
14	4988.0
15	4918.0
16	4848.0
17	4522.0
18	4196.0
19	4044.0
20	3892.0
21	3898.0
22	3904.0
23	3866.5
24	3829.0
25	4175.0
26	4856.0
27	5191.0
28	5979.0
29	6767.0
30	7481.5
31	8196.0
32	9238.5
33	10281.0
34	11848.0
35	13415.0
36	14403.0
37	15391.0
38	16371.5
39	17352.0
40	19563.0
41	21774.0
42	23581.5
43	25389.0
44	27346.0
45	29303.0
46	33195.5
47	37088.0
48	47095.0
49	57102.0
50	59084.5
51	61067.0
52	62172.5
53	63278.0
54	55225.5
55	47173.0
56	42139.0
57	37105.0
58	33850.0
59	30595.0
60	27841.0
61	25087.0
62	22375.0
63	19663.0
64	18384.5
65	17106.0
66	14669.0
67	12232.0
68	10533.0
69	8834.0
70	7667.0
71	6500.0
72	5689.5
73	4879.0
74	4197.0
75	2875.0
76	2235.0
77	1755.0
78	1275.0
79	957.0
80	639.0
81	468.0
82	297.0
83	216.5
84	136.0
85	105.0
86	74.0
87	50.5
88	27.0
89	17.0
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.41937393873777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14900057331377	28.834878068443988
2	8.334085088319643	6.570488330674608
3	3.887622867099691	4.597429785929717
4	2.5442701557751053	4.011741466866779
5	1.9125358968382553	3.7695483844363187
6	1.4715913185950689	3.480552508361952
7	1.2577567538316217	3.4705988662283147
8	1.0947908734301168	3.4524776659567284
9	0.9331333960086283	3.3105180844789555
>10	5.341461657401751	31.299435223893312
>50	0.044019172178129715	1.1758318724715646
>100	0.02432638407949654	1.7011128512482816
>500	0.0019306654031346429	0.6254359972959183
>1k	0.003089064645015429	1.7444930554900269
>5k	0.0	0.0
>10k+	3.861330806269286E-4	1.9554578382235732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12847	1.9331529639070546	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	2024	0.304561500657576	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	1960	0.2949310974747277	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	1584	0.2383524787754942	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1508	0.22691637499586192	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	1281	0.19275853870669704	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1090	0.1640178042078843	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	1007	0.15152837508012798	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1007	0.15152837508012798	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC	944	0.14204844694701171	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	869	0.13076281821711142	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	835	0.12564666652622328	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	819	0.12323906573051123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5047504973200393E-4	0.0	0.0	0.13121424336630744	0.0
2	1.5047504973200393E-4	0.0	0.0	0.6312428336257565	0.0
3	1.5047504973200393E-4	0.0	0.0	1.221556453724408	0.0
4	1.5047504973200393E-4	0.0	0.0	1.7056346887122646	0.0
5	1.5047504973200393E-4	0.0	0.0	2.8858105037603714	0.0
6	1.5047504973200393E-4	0.0	0.0	4.143781919519925	0.0
7	1.5047504973200393E-4	0.0	0.0	5.179050261676111	0.0
8	1.5047504973200393E-4	0.0	0.0	6.809597900572106	0.0
9	1.5047504973200393E-4	0.0	0.0	7.79280187552102	0.0
10	1.5047504973200393E-4	0.0	0.0	9.257526009612347	0.0
11	1.5047504973200393E-4	0.0	0.0	10.337936866688134	0.0
12	1.5047504973200393E-4	0.0	0.0	11.304137161017332	0.0
13	1.5047504973200393E-4	0.0	0.0	11.764891763296728	0.0
14	1.5047504973200393E-4	0.0	0.0	12.035144952615406	0.0
15	1.5047504973200393E-4	0.0	0.0	12.321348497205678	0.0
16	1.5047504973200393E-4	0.0	0.0	12.740271035659577	0.0
17	1.5047504973200393E-4	0.0	0.0	13.21501981756405	0.0
18	1.5047504973200393E-4	0.0	0.0	13.778849828909868	0.0
19	1.5047504973200393E-4	0.0	0.0	14.11606441535929	0.0
20	1.5047504973200393E-4	0.0	0.0	14.443197173476666	0.0
21	1.5047504973200393E-4	0.0	0.0	14.790493588258132	0.0
22	1.5047504973200393E-4	0.0	0.0	15.152987983062529	0.0
23	1.5047504973200393E-4	0.0	0.0	15.515331902817193	0.0
24	1.5047504973200393E-4	0.0	0.0	15.819592453375305	0.0
25	1.5047504973200393E-4	0.0	0.0	16.081569514958726	0.0
26	1.5047504973200393E-4	0.0	0.0	16.361001682311056	0.0
27	1.5047504973200393E-4	0.0	0.0	16.676397386549336	0.0
28	1.5047504973200393E-4	0.0	0.0	16.963955206587197	0.0
29	1.5047504973200393E-4	0.0	0.0	17.284467062516363	0.0
30	1.5047504973200393E-4	0.0	0.0	17.68713829559921	0.0
31	1.5047504973200393E-4	0.0	0.0	18.007499676478645	0.0
32	1.5047504973200393E-4	0.0	0.0	18.36412554434349	0.0
33	1.5047504973200393E-4	0.0	0.0	18.68463740027266	0.0
34	1.5047504973200393E-4	0.0	0.0	19.016735835031195	0.0
35	1.5047504973200393E-4	0.0	0.0	19.365837950409443	0.0
36	1.5047504973200393E-4	0.0	0.0	19.675967027907102	0.0
37	1.5047504973200393E-4	0.0	0.0	20.015438740102503	0.0
38	1.5047504973200393E-4	0.0	0.0	20.40727576960464	0.0
39	3.0095009946400785E-4	0.0	0.0	20.949888798938247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6925	0.0	43.440434	1
AGTACGG	60	3.6379788E-12	41.249996	2
ACGGGCT	155	0.0	39.19355	5
CCGATGA	305	0.0	37.62295	18
CGTTCTG	150	0.0	37.5	1
ACGTTAG	30	1.13940376E-4	37.499996	1
AACGATA	30	1.13940376E-4	37.499996	15
TGAATCG	30	1.13940376E-4	37.499996	19
ATACGAG	30	1.13940376E-4	37.499996	1
GTTTTTT	8325	0.0	37.27027	2
GCGCGAC	200	0.0	37.125	9
CGTTTCT	275	0.0	36.818184	1
ATTACGG	25	0.0021064705	36.0	2
GGATCGA	25	0.0021064705	36.0	8
TAGCACG	25	0.0021064705	36.0	1
CTACGGG	100	0.0	36.0	3
ATCGGGT	25	0.0021064705	36.0	4
CGATCAT	25	0.0021064705	36.0	10
ATCGCGC	25	0.0021064705	36.0	22
AGTCGAT	25	0.0021064705	36.0	43
>>END_MODULE