FastQCFastQC Report
Sat 18 Jun 2016
SRR3554395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627624
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116631.8582782047850306No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC8190.13049214179190088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58900.042.8989831
CGTTATT900.040.01
CACGGGA2150.037.674424
GCCGATA301.13933136E-437.5000049
CCGGTAT301.13933136E-437.50000442
AGTACGG552.744855E-936.8181842
ATATGCG250.002106381836.01
TACGGGT504.8728907E-836.04
GTATGCG250.002106381836.01
AACCTCG250.002106381836.020
GTTTTTT74750.035.78932
TAACGGG950.035.5263183
CTACGGG1350.035.0000043
ACACGGG1800.035.03
CAACGAG4050.035.014
TTGCGAG458.691568E-735.01
TGTAGCG458.691568E-735.01
TGCTACG458.691568E-735.01
ACAACGA4000.034.87513
CACAACG4050.034.44444712
Produced by FastQC (version 0.11.5)