FastQCFastQC Report
Sat 18 Jun 2016
SRR3554394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417867
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178484.271215482438192No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC7110.17014983236292888TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC4600.1100828732587163TruSeq Adapter, Index 22 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT406.7975634E-945.0000044
ACAACGG253.8865295E-545.02
ACGTAGG302.1621727E-644.9999962
CGTTTTT99650.043.938791
CGTTATT1450.043.4482731
CGCATGG650.041.538462
GCGCGAG403.4511868E-739.3750041
CGGGTCA356.23875E-638.5714266
ACGGGTC356.23875E-638.5714265
GTTTTTT115800.038.374352
ACGACCA2850.037.89473728
AACACGT2700.037.541
CGTGGCG301.1386789E-437.49999627
TTGTGCG301.1386789E-437.4999961
CACGAGG301.1386789E-437.4999962
AGCGACG301.1386789E-437.4999961
ATAACGC301.1386789E-437.49999611
TAGTGCG301.1386789E-437.4999961
ACACGAC2900.037.2413826
CACGACC2900.037.2413827
Produced by FastQC (version 0.11.5)