##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417867
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.005791316375785	33.0	31.0	34.0	30.0	34.0
2	32.142528603598755	34.0	31.0	34.0	30.0	34.0
3	32.08963617610388	33.0	31.0	34.0	30.0	34.0
4	35.7635491675581	37.0	35.0	37.0	35.0	37.0
5	35.265359552202014	37.0	35.0	37.0	33.0	37.0
6	35.437378400304404	37.0	35.0	37.0	33.0	37.0
7	35.91374288948398	37.0	35.0	37.0	35.0	37.0
8	35.788123972460134	37.0	35.0	37.0	35.0	37.0
9	37.67951046624883	39.0	38.0	39.0	35.0	39.0
10	37.29492158988386	39.0	37.0	39.0	34.0	39.0
11	37.39721729641249	39.0	37.0	39.0	34.0	39.0
12	37.417206910332716	39.0	37.0	39.0	35.0	39.0
13	37.44403123481874	39.0	37.0	39.0	35.0	39.0
14	38.60336183522509	40.0	38.0	41.0	34.0	41.0
15	38.75927029413665	40.0	38.0	41.0	35.0	41.0
16	38.57184702309586	40.0	38.0	41.0	34.0	41.0
17	38.577444497890475	40.0	38.0	41.0	34.0	41.0
18	38.20199728621786	39.0	38.0	41.0	34.0	41.0
19	37.70816073056738	38.0	37.0	41.0	34.0	41.0
20	37.4279375973695	39.0	35.0	41.0	34.0	41.0
21	37.29238250448109	39.0	35.0	41.0	33.0	41.0
22	37.35924349135012	39.0	35.0	41.0	33.0	41.0
23	37.386620623308374	39.0	35.0	41.0	34.0	41.0
24	37.251800213943675	39.0	35.0	41.0	33.0	41.0
25	36.98224794013406	38.0	35.0	41.0	33.0	41.0
26	37.07005578329947	38.0	35.0	41.0	33.0	41.0
27	37.062493568527785	38.0	35.0	41.0	33.0	41.0
28	36.8628343468137	38.0	35.0	41.0	33.0	41.0
29	36.72787035109257	38.0	35.0	41.0	33.0	41.0
30	36.27021277104916	38.0	35.0	41.0	31.0	41.0
31	35.938557004980055	38.0	35.0	41.0	30.0	41.0
32	35.32450516551917	38.0	35.0	41.0	24.0	41.0
33	34.5699062141782	38.0	35.0	41.0	18.0	41.0
34	33.904912328563874	38.0	34.0	41.0	15.0	41.0
35	33.51231372661636	38.0	33.0	41.0	10.0	41.0
36	33.21132082696169	38.0	33.0	41.0	10.0	41.0
37	33.133087800663844	38.0	33.0	41.0	10.0	41.0
38	33.00794511172215	38.0	33.0	41.0	10.0	41.0
39	32.858167790229906	38.0	33.0	41.0	10.0	41.0
40	32.75368717797768	38.0	32.0	41.0	10.0	41.0
41	32.58363785606425	37.0	32.0	40.0	10.0	41.0
42	32.54475227763858	37.0	32.0	40.0	9.0	41.0
43	32.43385096214824	37.0	31.0	40.0	8.0	41.0
44	32.34223329432571	37.0	31.0	40.0	8.0	41.0
45	32.246643070642094	37.0	31.0	40.0	8.0	41.0
46	32.1070316631847	36.0	31.0	40.0	8.0	41.0
47	32.050841535703945	36.0	31.0	40.0	8.0	41.0
48	31.987376366164355	36.0	31.0	40.0	8.0	41.0
49	31.92071879330026	36.0	31.0	40.0	8.0	41.0
50	31.794145027006202	36.0	30.0	40.0	8.0	41.0
51	31.119679228079747	35.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	11.0
11	10.0
12	9.0
13	13.0
14	20.0
15	35.0
16	63.0
17	118.0
18	240.0
19	442.0
20	743.0
21	1154.0
22	1766.0
23	3061.0
24	5486.0
25	10094.0
26	15220.0
27	16988.0
28	15402.0
29	12589.0
30	10356.0
31	9884.0
32	10276.0
33	13140.0
34	21972.0
35	27619.0
36	29566.0
37	37667.0
38	66337.0
39	107500.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.40655998200384	14.263868647201141	17.013308062134602	32.31626330866041
2	49.84026017847784	17.936329023349533	18.047847760172495	14.17556303800013
3	25.899389040053418	18.95148456327013	42.03035894196	13.118767454716455
4	22.040266400553286	20.3040680407881	42.280199202138476	15.375466356520137
5	20.434013693352192	23.77502889675423	41.105423495992746	14.685533913900834
6	20.48211512275437	26.743916126423002	41.24853123122907	11.525437519593556
7	62.42105741779083	4.774964282893839	29.48521898115908	3.318759318156256
8	62.89130273508078	4.648368978646316	28.969504651001394	3.4908236352715094
9	57.80643123290425	5.896613037162543	31.115402747764243	5.181552982168967
10	29.13438965029543	21.607353535933683	36.76145759296618	12.496799220804705
11	24.231394199589822	21.622669413952288	40.92019709620525	13.225739290252639
12	20.352169470190276	20.6458035690782	44.13198457882532	14.870042381906204
13	20.307179078510625	19.85966826765454	45.300777520120036	14.532375133714796
14	17.947576621269448	20.972701840537777	45.60900956524445	15.470711972948331
15	17.675001854657104	19.53253068560092	46.88573158445151	15.906735875290462
16	20.863097588467145	20.98371012786365	43.71295172866007	14.44024055500913
17	20.751818162238227	19.98506701893186	43.59257849985761	15.670536318972307
18	22.886229350487117	18.030378086807524	43.981697525767764	15.10169503693759
19	20.09251747565613	20.70156293748968	42.928491601394704	16.27742798545949
20	21.483869269408686	20.87506311816918	42.92490194248409	14.716165669938041
21	20.21049759851819	22.283645274692667	43.5966467799563	13.909210346832843
22	20.145883738127203	19.715603290042047	44.00945755467649	16.129055417154262
23	18.082547796308397	22.29537149380066	44.15400115347706	15.468079556413883
24	17.58621762426801	20.832465832429936	44.830292892236045	16.751023651066006
25	18.33813151074385	22.113256131735696	43.460000430759074	16.088611926761388
26	18.293619740252282	20.863576209655225	43.63517578559685	17.207628264495643
27	18.846905833674352	19.8723517291387	43.92713471032649	17.35360772686046
28	16.922848657587224	22.357113627063157	43.753634529646995	16.966403185702628
29	18.26179143124487	21.941431125214482	43.391796911457476	16.404980532083176
30	19.446857492934356	20.55989106581759	44.03314930348652	15.960102137761536
31	20.01904912328564	21.6492328898908	41.93511332553181	16.39660466129175
32	19.569145206489146	21.926354557789917	41.760416591882105	16.744083643838827
33	19.880248978742042	22.415266101415042	38.92195363596551	18.782531283877407
34	19.65410046737359	24.06674851088983	39.407036210085984	16.872114811650597
35	19.961853891309914	24.823209298652444	37.632548155274286	17.58238865476336
36	20.84826033163662	26.995431560759762	34.679694735406244	17.476613372197374
37	20.539788975918174	26.33565225298959	33.842825588045955	19.281733183046278
38	21.531731388216823	26.11093960518538	33.8765683818057	18.4807606247921
39	21.981874615607357	24.043296072673844	33.57886600281908	20.395963308899724
40	23.80685720576164	23.39835402173419	34.713916150354066	18.08087262215011
41	20.891575549157988	24.670289829060444	33.76696412973506	20.67117049204651
42	22.099615427875378	24.591317333027014	33.97875400546107	19.330313233636538
43	21.42906714337337	24.580069735107106	34.27980673276425	19.711056388755274
44	21.506603775842553	24.439355105811178	33.71120476132358	20.34283635702269
45	20.88702864787121	23.69438122656252	34.269994998408585	21.148595127157684
46	20.751578851644183	24.352963981362493	34.11994725594507	20.77550991104825
47	20.020006365661803	25.09195509576013	35.53858045741827	19.349458081159796
48	20.125542337633746	25.141253078132515	35.131752447549104	19.60145213668464
49	21.344590503677008	24.07105610158256	34.87042527885667	19.71392811588376
50	19.13551441008742	24.466636513531817	35.89539255313293	20.50245652324783
51	20.12362785288142	23.15521445818885	34.594739474521795	22.126418214407934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	464.0
2	732.0
3	15170.0
4	29608.0
5	19201.0
6	8794.0
7	8418.0
8	8042.0
9	7944.5
10	7847.0
11	7708.5
12	7570.0
13	7419.0
14	7268.0
15	6851.0
16	6434.0
17	6004.5
18	5575.0
19	5251.0
20	4927.0
21	4664.5
22	4402.0
23	4221.5
24	4041.0
25	4035.5
26	4295.0
27	4560.0
28	4921.0
29	5282.0
30	5942.0
31	6602.0
32	7204.5
33	7807.0
34	8790.0
35	9773.0
36	10091.5
37	10410.0
38	11677.5
39	12945.0
40	13606.5
41	14268.0
42	15378.5
43	16489.0
44	17647.0
45	18805.0
46	19876.5
47	20948.0
48	23869.5
49	26791.0
50	27989.0
51	29187.0
52	29885.0
53	30583.0
54	28198.5
55	25814.0
56	22870.5
57	19927.0
58	17672.0
59	15417.0
60	13762.5
61	12108.0
62	10883.5
63	9659.0
64	8470.5
65	7282.0
66	6048.0
67	4814.0
68	4005.5
69	3197.0
70	2592.0
71	1987.0
72	1711.0
73	1435.0
74	1226.0
75	894.0
76	771.0
77	537.0
78	303.0
79	211.5
80	120.0
81	100.0
82	80.0
83	47.5
84	15.0
85	9.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.029178874085346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48162557542936	37.76283747619891
2	8.574296075823854	8.579299841935192
3	4.549234115261549	6.827833418775342
4	3.0902685509788483	6.184143924235253
5	2.2302628954100205	5.578911066535171
6	1.6479410683218416	4.946708308861482
7	1.2270475345687644	4.297172641576229
8	0.928621941651873	3.7166554580241593
9	0.6403436937034472	2.883228227386405
>10	1.5621201625723122	10.847908530399074
>50	0.03865251250722834	1.3242630190834626
>100	0.028631490745998772	2.6203553087115155
>500	4.7719151243331287E-4	0.16974058167765008
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.7719151243331287E-4	4.260942196600139
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17848	4.271215482438192	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	711	0.17014983236292888	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	460	0.1100828732587163	TruSeq Adapter, Index 22 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3931059404068758E-4	0.0	0.0	0.07969042781554897	0.0
2	2.3931059404068758E-4	0.0	0.0	0.30153134849126634	0.0
3	2.3931059404068758E-4	0.0	0.0	0.47574946095288695	0.0
4	2.3931059404068758E-4	0.0	0.0	0.6755738069768611	0.0
5	2.3931059404068758E-4	0.0	0.0	1.2094757422816351	0.0
6	2.3931059404068758E-4	0.0	0.0	1.7905218646124246	0.0
7	2.3931059404068758E-4	0.0	0.0	2.261006492496416	0.0
8	2.3931059404068758E-4	0.0	0.0	2.9786989640244386	0.0
9	2.3931059404068758E-4	0.0	0.0	3.4183125252771815	0.0
10	2.3931059404068758E-4	0.0	0.0	4.177884350762324	0.0
11	2.3931059404068758E-4	0.0	0.0	4.8364671055622965	0.0
12	2.3931059404068758E-4	0.0	0.0	5.43306841650573	0.0
13	2.3931059404068758E-4	0.0	0.0	5.78437636855746	0.0
14	2.3931059404068758E-4	0.0	0.0	5.981568298046986	0.0
15	2.3931059404068758E-4	0.0	0.0	6.177324363972269	0.0
16	2.3931059404068758E-4	0.0	0.0	6.475266053552925	0.0
17	2.3931059404068758E-4	0.0	0.0	6.778951197390557	0.0
18	2.3931059404068758E-4	0.0	0.0	7.150361239341704	0.0
19	2.3931059404068758E-4	0.0	0.0	7.380817341402887	0.0
20	2.3931059404068758E-4	0.0	0.0	7.621803109601859	0.0
21	2.3931059404068758E-4	0.0	0.0	7.87977992997772	0.0
22	2.3931059404068758E-4	0.0	0.0	8.159534014411284	0.0
23	2.3931059404068758E-4	0.0	0.0	8.42612601617261	0.0
24	2.3931059404068758E-4	0.0	0.0	8.656582118233793	0.0
25	2.3931059404068758E-4	0.0	0.0	8.887277530889016	0.0
26	2.3931059404068758E-4	0.0	0.0	9.120844670672726	0.0
27	2.3931059404068758E-4	0.0	0.0	9.38312908174132	0.0
28	2.3931059404068758E-4	0.0	0.0	9.615021047366746	0.0
29	2.3931059404068758E-4	0.0	0.0	9.865339928733306	0.0
30	2.3931059404068758E-4	0.0	0.0	10.22191271385393	0.0
31	2.3931059404068758E-4	0.0	0.0	10.49400885927819	0.0
32	2.3931059404068758E-4	0.0	0.0	10.843641637171636	0.0
33	2.3931059404068758E-4	0.0	0.0	11.16120679546363	0.0
34	2.3931059404068758E-4	0.0	0.0	11.456276757915797	0.0
35	2.3931059404068758E-4	0.0	0.0	11.759961901753428	0.0
36	2.3931059404068758E-4	0.0	0.0	12.042109092127399	0.0
37	2.3931059404068758E-4	0.0	0.0	12.319230760026516	0.0
38	2.3931059404068758E-4	0.0	0.0	12.66216284128682	0.0
39	4.7862118808137516E-4	0.0	0.0	13.129775742042325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	40	6.7975634E-9	45.000004	4
ACAACGG	25	3.8865295E-5	45.0	2
ACGTAGG	30	2.1621727E-6	44.999996	2
CGTTTTT	9965	0.0	43.93879	1
CGTTATT	145	0.0	43.448273	1
CGCATGG	65	0.0	41.53846	2
GCGCGAG	40	3.4511868E-7	39.375004	1
CGGGTCA	35	6.23875E-6	38.571426	6
ACGGGTC	35	6.23875E-6	38.571426	5
GTTTTTT	11580	0.0	38.37435	2
ACGACCA	285	0.0	37.894737	28
AACACGT	270	0.0	37.5	41
CGTGGCG	30	1.1386789E-4	37.499996	27
TTGTGCG	30	1.1386789E-4	37.499996	1
CACGAGG	30	1.1386789E-4	37.499996	2
AGCGACG	30	1.1386789E-4	37.499996	1
ATAACGC	30	1.1386789E-4	37.499996	11
TAGTGCG	30	1.1386789E-4	37.499996	1
ACACGAC	290	0.0	37.24138	26
CACGACC	290	0.0	37.24138	27
>>END_MODULE